Molecular cloning and sequence characterization of two genes, DQA1 and DQA2, from the Chinese yakow ( Bos grunniens × Bos taurus )
The major histocompatibility complex (MHC) plays a crucial role in the immune response to infectious diseases. The aim of the present study was to investigate the structural and functional characteristics and possible duplication of the MHC-DQA genes in the Chinese yakow, which is well adapted to the Qinghai?Tibetan Plateau. Two full-length cDNA sequences and partial 5'- and 3'-untranslated regions (UTRs) were retrieved and denoted as Bogr×BoLA-DQA*0101 (DQA1) and BogrxBoLA-DQA*2001 (DQA2), respectively. The percentage of nucleotide and amino acid sequences homology between BogrxBoLA-DQA1 and BogrxBoLA-DQA2 showed that these two sequences have more identity to alleles of the corresponding DQA1 and DQA2 genes from other counterparts than to each other. Moreover, the Bogr×BoLA-DQA1 and -DQA2 exhibited considerable variation with 99 nucleotide polymorphisms along the length of the coding regions, leading to 52 amino acid polymorphisms. The phylogenetic tree investigation also demonstrated that there is a larger genetic gap within these two identified genes than within orthologous genes from different animals, suggesting that these sequences belong to nonallelic and duplicated types in the Chinese yakow. This is the first report to isolate and identify the MHC-DQA1 and -DQA2 genes. This result will deepen our understanding of MHC diversity among different ruminant species based on comparative population genetics.
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- Park YH, Joo YS, Park JY, Moon JS, Kim SH, Kwon NH, Ahn
JS, Davis WC, Davies CJ. Characterization of lymphocyte
subpopulations and major histocompatibility complex
haplotypes of mastitis-resistant and susceptible cows. J Vet Sci
2004; 5: 29-39.
- Castillo S, Srithayakumar V, Meunier V, Kyle CJ.
Characterization of major histocompatibility complex (MHC)
DRB exon 2 and DRA exon 3 fragments in a primary terrestrial
rabies vector (
Procyon lotor
). PLoS ONE 2010; 5: e12066.
- Ellis SA, Codner G. The impact of MHC diversity on cattle T
cell responses. Vet Immunol Immunopathol 2012; 148: 74-77.
- Sin YW, Dugdale HL, Newman C, Macdonald DW,
Burke T. MHC class II genes in the European badger
(
Meles meles
): characterization, patterns of variation, and
transcription analysis. Immunogenetics 2012; 64: 313-327.
- Hughes AL, Yeager M. Natural selection at major
histocompatibility complex loci of vertebrates. Annu Rev
Genet 1998; 32: 415-435.
- Gelhaus A, Förster B, Wippern C, Horstmann RD. Evidence for
an additional cattle DQA locus, BoLA-DQA5. Immunogenetics
1999; 49: 321-327.
- Hameed KGA, Sender G, Maynt M. Major histocompatibility
complex polymorphism and mastitis resistance – a review.
Anim Sci Pap Rep 2006; 24: 11-25.
- Miyasaka T, Takeshima SN, Matsumoto Y, Kobayashi
N, Matsuhashi T, Miyazaki Y, Tanabe Y, Sentsui H, Aida Y. The
diversity of bovine MHC class II DRB3 and DQA1 alleles in
different herds of Japanese Black and Holstein cattle in Japan.
Gene 2011; 472: 42-49.
- Kappes D, Strominger JL. Human class II major
histocompatibility complex genes and proteins. Annu Rev
Biochem 1988;
57: 991-1028.
- Andersson L, Rask L. Characterization of the MHC class
II region in cattle: the number of DQ genes varies between
haplotypes. Immunogenetics 1988; 27: 110-120.
- Sigurdardottir S, Borsch C, Gustafsson K, Andersson L. Gene
duplications and sequence polymorphism of bovine class II
DQB genes. Immunogenetics 1992; 35: 205-213.
Fries R, Hediger R, Stranzinger G. Tentative chromosomal
localization of the bovine major histocompatibility complex by
in situ
hybridization. Anim Genet 1986; 17: 287-294.
- Russell BC, Gallagher A, Craigmile S, Glass EJ. Characterization
of cattle cDNA sequences from two DQA loci. Immunogenetics
1997; 45: 455-458.
- Xi D, Wu M, Fan Y, Huo Y, Leng J, Gou X, Mao
H, Deng W (2012). Isolation and characteristics of
the
melanocortin
1
receptor
gene
(
MC1R
) in
the Chinese yakow (
Bos grunniens × Bos taurus
). Gene 498:
259-263.
- Xi D, Hao T, He Y, Leng J, Sun Y, Yang Y, Mao H, Deng W.
Nucleotide sequence and polymorphism of MHC class II DQB
exon 2 alleles in Chinese yakow (
Bos grunniens × Bos taurus
).
Int J Immunogenet 2014; 41: 269-275.
- Niranjan SK, Deb SM, Sharma A, Kumar S, Mitra A. Isolation
of two cDNAs encoding MHC-DQA1 and DQA2 from the
water buffalo
Bubalus bubalis
. Vet Immunol Immunopathol
2009; 130: 268-271.
- Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular
evolutionary genetics analysis (MEGA) software version 4.0.
Mol Biol Evol 2007; 24: 1596-1599.
- Germain RN, Margulies DH. The biochemistry and cell biology
of antigen processing and presentation. Annu Rev Immunol
1993; 11: 403-450.
- Williams MA, Trout R, Spector SA. HIV-1 gp120 modulates
the immunological function and expression of accessory and
co-stimulatory molecules of monocyte-derived dendritic cells.
J Hematother Stem Cell Res 2002; 11: 829-847.
- Ballingall KT, Marasa BS, Luyai A, McKeever DJ. Identification
of diverse BoLA DQA3 genes consistent with non-allelic
sequences. Anim Genet 1998; 29: 123-129.
- Sun Y, Zheng H, Xi D, Zhang X, Du M, Pu L, Lin M, Yang
Y. Molecular characteristics of the MHC-DRA genes from
yak (
Bos grunniens
) and Chinese yakow (
Bos grunniens × Bos
taurus
). Int J Immunogenet 2014; 41: 69-73.
- Tamate SC, Kawata M, Makino T. Contribution of
nonohnologous duplicated genes to high habitat variability in
mammals. Mol Biol Evol 2014; 31: 1779-1786.
- Brawand D, Wagner CE, Li YI, Malinsky M, Keller I, Fan S,
Simakov O, Ng AY, Lim ZW, Bezault E et al. The genomic
substrate for adaptive radiation in African cichlid fish. Nature
2014; 513: 375-381.