Miling MA, Zhijie LIU, Guiquan GUAN, Youquan LI, Jianxun LUO, Hong YIN

Molecular and serological evidence for Anaplasma infection in yaks

Bovine anaplasmosis caused by Anaplasma marginale is considered to be an important disease in China; large-scale investigations have not been carried out since the 1990s, however, and whether or not yaks can be infected by A. marginale has never been investigated. In this study, 2 yaks were successfully infected by the inoculation of blood infected with A. marginale under experimental conditions. Furthermore, an investigation of 482 field serum samples from yaks using an enzyme-linked immunosorbent assay (ELISA) with the recombinant major surface protein 5 (MSP5) showed that 84.4% of the results were positive. In consideration of previously determined ecological and biological data related to A. marginale, it is assumed that yaks are potential natural reservoir hosts of A. marginale in the northwest of China.

Anahtar Kelimeler:

Key words: Yak, Anaplasma marginale, ELISA, MSP5

Molecular and serological evidence for Anaplasma infection in yaks

Keywords:

Key words: Yak, Anaplasma marginale, ELISA, MSP5,

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Palmer, G.H., Barbet, A.F., Kuttler, K.L.: Detection of an Anaplasma marginale common surface protein present in all stages of infection. J. Clin. Microbiol., 1986; 23: 1078-1083.
Ajayi, S.A., Wilson, A.J., Campbell, R.S.: Experimental bovine anaplasmosis: clinico-pathological and nutritional studies. Res. Vet. Sci., 1978; 25: 76-81.
Lu, W.X., Lu, W.S., Zhang, Q.C.: Epidemiology investigation and distribution character of bovine piroplasmosis. Chin. J. Vet. Sci. Technol., 1995; 25: 13-16 (in Chinese). 4. Yang, P.: Proceedings of 1st National Conference on Veterinary Parasitology in China, Vol. 1. Northwestern College of Animal Science and Veterinary Medicine, Lanzhou. 1964; 346-351 (in Chinese).
Zhang, Q.C., Lu, W.S., Dou, H.F.: Studies on complement fi xation test for diagnosis of Anaplasma marginale infection in cattle. Chin. J. Vet. Sci. Technol., 1993; 23: 8-10 (in Chinese).
Yu, F., Lu, W.S., Zhang, Q.C.: Indirect fl uorescent antibody diagnostic anaplasmosis research. Chin. J. Vet. Sci. Technol., 1990; 20: 8-10 (in Chinese).
Ma, M.L., Luo, J.X., Yin, H.: Cloning and prokaryotic expression of MSP5 protein-encoding gene of Anaplasma marginale. Chin. Vet. Sci., 2007; 37: 578-582 (in Chinese).
Ma, M.L., Yin, H., Guan, G.Q.: Development of an indirect ELISA based on the MSP5 recombinant antigen of Anaplasma marginale. Chin. Vet. Sci., 2008; 38: 641-645 (in Chinese). 9. de la Fuente, J., Atkinson, M. W., Naranjo, V.: Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet. Microbiol., 2007; 119: 375-381.
Li, H.H., He, D.S., Ou-Yang, X.X.: Survey on Anplasma infection in cattle in Hunan. Chinese J. Vet. Parasitol., 2008; 16: 9-15 (in Chinese).
Liu, G.Q.: Anaplasmosis outbreak in cattle and the diagnosis and treatment. Hunan J. Anim. Sci. Vet. Med., 2008; 3: 20-21 (in Chinese).
Wang, Y.X., Yuan, Q.M., Long, P.R.: Bovine anaplasmosis and eperythrozoonosis diagnostic report. Yunnan J. Anim. Sci. Vet. Med., 2003; 4: 35 (in Chinese).
Kocan, K.M., de la Fuente, J., Blouin, E.F.: Th e natural history of Anaplasma marginale. Vet. Parasitol., 2010; 167: 95-107.
Bai, Q., Chen, Z. H., Ying, S. X.: Study on isolation and preservation of single species of hematocytozoon in bovine – separation of bovine A. marginale single isolate. Chin. J. Vet. Sci. Technol., 1987; 3:12-15 (in Chinese).
Bai, Q., Liu, G.Y., Zhang, L.: Study on isolation and preservation of single species of hematocytozoon in bovine. Sci. Agric. Sin., 1992; 25: 83-88 (in Chinese).
Jiang, X. S., Huang, X. F. Yak Parasitosis, 1st ed. 1996 (in Chinese).