Bu çal›flmada, farkl› dozlardaki linamarin, amigdalin ve gossipol asetik asit’in in vitro olarak bo¤a spermi hyaluronidaz aktivitesi, motilitesi ve anormal spermatozoa yüzdeleri üzerine olan etkileri araflt›r›ld›. Çal›flmada 2 ile 3 yafllar› aras›nda 12 adet Holfltayn ›rk› bo¤a kullan›ld›. ‹nkubasyonlar için kullan›lacak olan sperma örnekleri her hayvandan üç kez al›nd›. Numuneler 5 eflit k›sma ayr›ld› ve 1:1 (v/v) oran›nda, 0,5, 0,75, 1 ve 2 µM linamarin, 0,4, 0,8, 1 ve 2 µM amigdalin ve 2,5, 5, 10 ve 20 µM gossipol asetik asit solusyonlar›yla kar›flt›r›ld›. Bu çal›flmada hyaluronik asitten son ürün olarak sakkaritler ve N-asetilglukozamin ayr›flmas› esas›na dayanan kolorimetrik metot kullan›ld›. Sperm motilitesi ve morfolojisi ise faz-kontrast mikroskop yard›m›yla tayin edildi. Üç bilefli¤in bo¤a spermi ile inkübasyonu, hyaluronidaz aktiviteleri (P < 0,01) ile sperm motilitelerinde (P < 0,001) kontrol grubuna göre önemli oranda azalmalara neden oldu. Ayr›ca, gossipol asetik asit, inkübasyon periyodunda anormal spermatozoa oran›n› da önemli (P < 0,001) oranda art›rd›. Bu sonuçlar, linamarin, amigdalin ve gossipol asetik asit’in bo¤a spermi üzerinde doza ba¤›ml› olarak zararl› etkilere neden oldu¤unu göstermektedir.

In vitro effects of various doses of linamarin, amygdalin and gossypol acetic acid on sperm hyaluronidase activity and the percentages of motile spermatozoa and morphology were investigated in bull sperm. Holstein bulls (n = 12) aged between 2 and 3 were used in the study. Semen samples were collected from each animal 3 times and used for incubation. The samples were divided into 5 equal parts and mixed (v/v) with linamarin at doses of 0.5, 0.75, 1 and 2 µM, with amygdalin at doses of 0.4, 0.8, 1 and 2 µM and with gossypol acetic acid at doses of 2.5, 5, 10 and 20 µM. The colorimetric method based on the liberation of saccharides with N-acetylglucosamine end &#8211; groups from hyaluronic acid was used for the determination of semen hyaluronidase activity in this study. Sperm motility and morphology were assessed under a phase-contrast microscope. The incubation of the 3 compounds with sperm caused significant decreases in spermatozoa motility (P < 0.001) and hyaluronidase activity (P < 0.01) compared with the control group. Additionally, gossypol acetic acid produced a significant (P < 0.001) increase in the rate of abnormal spermatozoa during the incubation period. These results showed that linamarin, amygdalin and gossypol acetic acid have deleterious effects on bull spermatozoa in a dose - dependent manner.