Hossam ABOUSHAARA, Martin STARON, Tatiana ČERMÁKOVÁ

Impacts of oxalic acid, thymol, and potassium citrate as Varroa control materials on some parameters of honey bees

Various treatments are currently available for the control of Varroa mites. Some of these treatments depend on using natural and nonchemical compounds. In this study, the impacts of some materials (oxalic acid, thymol, and potassium citrate) on the survival of honey bees and some quality parameters were investigated under laboratory conditions. The potential impacts of different feeding types (sugar syrup, sugar candy, honey candy, honey jelly, and creamed honey) mixed with the tested Varroa control materials on honey bees were also studied. The study showed that using high percentages of oxalic, thymol, or potassium citrate can passively impact honey bees while using 0.5% is more preferable. The study also proved that feeding type, either as a liquid or solid, except for honey jelly, has no undesirable impacts on the survival or studied parameters of honey bee workers and drones. Drones fed on potassium regardless of feeding type were able to survive significantly at day 4 more so than drones fed on oxalic acid or thymol. Investigations into the role of potassium in enhancing the survival of bee drones and in controlling Varroa mites are recommended.

Keywords:

Honey bees, Va r r o a thymol, oxalic acid, potassium,

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Chen Y, Pettis JS, Evans JD, Kramer M, Feld laufer MF. Transmission of Kashmir bee virus by the ectoparasitic mite Varroa destructor . Apidologie 2004; 35: 441-448.
Rosenkranz P, Aumeier P, Ziegelmann B. Biology and control of Varroa destructor . J Invert Pathol 2010; 103: 96-119.
Abou-Shaara HF. Continuous management of Va r r o a mite in honey bee, Apis mellifera , colonies. Acarina 2014; 22: 149-156.
Škerl MIS, Nakrst M, Žvokelj L, Gregorc A. The acaricidal effect of flumethrin, oxalic acid and amitraz against Va r r o a destructor in honey bee ( Apis mellifera carnica ) colonies. Acta Vet Brno 2011; 80: 51-56.
Akyol E, Yeninar H. Use of oxalic acid to control Va r r o a destructor in honeybee ( Apis mellifera L.) colonies. Turk J Vet Anim Sci 2009; 33: 285-288.
Toomemaa K, Martin AJ, Mänd M, Williams IH. Using oxalic acid in water solution in control of Varroa mites and its influence on honey bees. Agron Res 2010; 8: 345-350.
Gregorc A, Planinc I. Dynamics of falling Va r r o a mites in honeybee ( Apis mellifera ) colo nies following oxalic acid treatments. Acta Vet Brno 2004; 73: 385-391.
Higes M, Meana A, Suárez M, Llorente J. Negative long-term effects on bee colonies treated with oxalic acid against Va r r o a jacobsoni Oud. Apidologie 1999; 30: 289-292.
Mahmood R, Wagchoure ES, Raja S, Sarwar G. Control of Varroa destructor using ox alic acid, formic acid and bayvarol strip in Apis mellifera (Hymenoptera: Apidae) colonies. Pak J Zool 2012; 44: 1473-1477.
Rashid M, Wagchoure ES, Mohsin AU, Raja S, Sarwar G. Control of ectoparasitic mite Varroa destructor in honeybee ( Apis mellifera L.) colonies by using different concentrations of oxalic acid. J Anim Plant Sci 2012; 22: 72-76.
Cornelissen B, Gerritsen L. Swarm preven tion and spring treatments against Varroa destruc tor in honey bee colonies ( Apis mellifera ). Pro c Neth Entomol Soc Meet 2006; 17: 133- 139.
Al-Zarog AA, El-Bassiouny AM. Influ ence of some plant extracts on Va r r o a mite and performance of honey bee Apis mellifera colonies. Egypt Acad J Biol Sci 2013; 5: 15-20.
Chiesa F. Effective control of varroatosis using powdered thymol. Apidologie 1991; 22: 135-145.
Imdorf A, Bogdanov S, Ochoa RI, Calderone NW. Use of essential oils for the control of Varroa jacobsoni Oud. in honey bee colonies. Apidologie 1999; 30: 209-228.
Marinelli E, Formato G, Vari G, De Pace FM. Varroa control using cellulose strips soaked in oxalic acid water solution. Apiacta 2006; 41: 54-59.
Mutinelli F, Baggio A, Capolongo F, Piro R, Pran din L, Biasion L. A scientific note on oxalic acid by topical application for the control of varroosis. Apidologie 1997; 28: 461-462.
Gregorc A, Planinc I. Use of thymol formulations, amitraz, and oxalic acid for the control of the Va r r o a mite in honey bee ( Apis mellifera carnica ) colonies. J Apic Sci 2012; 56: 61-69.
Costa C, Lodesani M, Maistrello L. Effect of thymol and resveratrol administered with candy or syrup on the development of Nosema ceranae and on the longevity of honeybees ( Apis mellifera L.) in laboratory conditions. Apidologie 2010; 41: 141-150.
Barker RJ. Some carbohydrates found in pollen and pollen substitutes are toxic to honey bees. J Nutr 1977; 107: 1859- 1862.
Abd Elhamid AM, Abou-Shaara HF. Producing clover and cotton creamed honey under cooling conditions and potential use as feeding to honey bee colonies. J Apic 2016; 31: 135-142.
Pileckas V, Švirmickas GJ, Razmaitė V, Paleck aitis M. Efficacy of different ecological methods for honeybee ( Apis mellifera ) Varroa pre vention in spring. Vet Med Zoot 2012; 59: 65-70.
Shouky RS, Khattaby AM, El-Sheakh AA, Abo-Ghalia AH, Elbanna SM. Effect of some materials for controlling Va r r o a mite on the honeybee drones ( Apis mellifera L.). Egypt J Agric Res 2013; 91: 825-834.
Charpentier G, Vidau C, Ferdy JB, Tabart J, Vetillard A. Lethal and sub-lethal effects of thymol on honeybee ( Apis mellifera ) larvae reared in vitro. Pest Manag Sci 2014; 70: 140-147.
Abou-Shaara HF. Thermal tolerance characteristics of two honey bee races. J Agr Urban Entomol 2015; 31: 1-8.
Hess G. Über den Einfluß derWeisellosigkeit und des Fruchtbarkeitsvitamins E auf die Ovarien der Bienenarbeiterin. Beihefte zur Schweizerischen Bienen-Zeitung 1942; 1: 33-110 (in German).
Mattila HR, Otis GW, Daley J, Schulz T. Trials of Apiguard, a thymol-based miticide part 2. Non-target effects on honey bees. Am Bee J 2000; 140: 68-70.
Waller GD. Evaluating responses of honey bees to sugar solutions using an artificial-flower feeder. Ann Entomol Soc Am 1972; 65: 857-862.
Al Toufailia H, Scandian L, Ratnieks FL. Towards integrated control of Va r r o a : 2) Comparing application methods and doses of oxalic acid on the mortality of phoretic Va r r o a destructor mites and their honey bee hosts. J Apic Res 2015; 54: 108-120.
Strachecka A, Paleolog J, Olszewski K, Borsuk G. Influence of amitraz and oxalic acid on the cuticle proteolytic system of Apis mellifera L. workers. Insects 2012; 3: 821-832.