Immune Responses in Mice Immunized Intranasally With Sendai Virus
Mice were immunized 3 times intranasally (i.n.) at intervals of 2 weeks with a formalin-inactivated Sendai virus (SV) whole virus with and without whole formalin-inactivated Bordetella pertussis (Bp) as adjuvant. The antibody response to SV was measured using the hemagglutination inhibition (HI) test in serum, and the IgA and IgG antibodies were evaluated using enzyme linked immunosorbent assay in saliva and serum. It was demonstrated that repeating immunizations with SV plus Bp could induce HI serum antibodies, IgA and serum IgG antibodies. However, in mice immunized subcutaneously with SV alone, high serum IgG antibodies and HI titers were induced only. In contrast, the animals immunized i.n. with SV alone had significantly lower titers to SV than those of the other groups.
Immune Responses in Mice Immunized Intranasally With Sendai Virus
Mice were immunized 3 times intranasally (i.n.) at intervals of 2 weeks with a formalin-inactivated Sendai virus (SV) whole virus with and without whole formalin-inactivated Bordetella pertussis (Bp) as adjuvant. The antibody response to SV was measured using the hemagglutination inhibition (HI) test in serum, and the IgA and IgG antibodies were evaluated using enzyme linked immunosorbent assay in saliva and serum. It was demonstrated that repeating immunizations with SV plus Bp could induce HI serum antibodies, IgA and serum IgG antibodies. However, in mice immunized subcutaneously with SV alone, high serum IgG antibodies and HI titers were induced only. In contrast, the animals immunized i.n. with SV alone had significantly lower titers to SV than those of the other groups.
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- Burek, J.D., Zurcher, C., Van Nunen, M.C., Hollander, C.F.: A naturally occurring epizootic caused by Sendai virus in breeding and aging rodent colonies. II. Infection in the rat. Lab. Anim. Sci., 1977; 27: 963-971.
- Ishida, N., Homma, M.: Sendai virus. Advan. Virus Res., 1978; 23: 349-383.
- Liang, S.C., Simecka, J.W., Lindsey, J.R., Cassell, G.H., Davis, J.K.: Antibody responses after Sendai virus infection and their role in upper and lower respiratory tract disease in rats. Lab. Anim. Sci., 1999; 49: 385-394. 4. Chanock, R.M., Murphy, B.R., Collins, P.L.: Parainfluenza viruses. In: Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., Strauss, S.E., Eds. Field’s Virology. Lippincott, Williams & Wilkins, Philadelphia, 2001; 341-1379.
- Simmons, J.H., Purdy, G.A., Franklin, C.L., Churchill, A.E., Russell, R.J., Besch-Wiliford, C.L., Riley, L.K.: Characterization of a novel parainfluenza virus, caviid parainfluenza virus 3, from laboratory guinea pigs (Cavia porcellus). Comp. Med., 2002; 52: 548-554.
- Janke, B.H., Paul, P.S., Landgraf, J.G., Halbur, P.G., Huinker, C.D.: Paramyxovirus infection in pig with interstitial pneumonia and encephalitis in the United States. J. Vet. Diagn. Invest., 2001; 13: 428-433.
- Ito, H., Matumoto, M.: Protective efficacy of intranasal vaccination with subunit Sendai virus vaccine in mice. Jpn. J. Exp. Med., 1986; 56: 125-129.
- Miyamae, T.: Protective effects of nasal immunization in mice with various kinds of inactivated Sendai virus vaccines. Microbiol. Immunol., 1986; 30: 213-223.
- Miyamae, T.: Selection of the most efficacious of twenty-two inactivated Sendai virus nasal vaccines by determination of the protection index in mice. Lab. Anim. Sci., 1999; 49: 149-152.
- Walker, R.I.: New strategies for using mucosal vaccination to achieve more effective immunization. Vaccine, 1994; 12: 387- 400.
- Berstad, A.K.H., Andersen, S.R., Dalseg, R., Drİmtorp, S., Holst, J., Namork, E., Wedege, E., Haneberg, B.: Inactivated meningococci and pertussis bacteria are immunogenic and act as mucosal adjuvants for a nasal inactivated influenza virus vaccine. Vaccine, 2000; 18: 1910-1919.
- Renegar, K.B., Small. P.A. Jr.: Immunoglobulin A mediation of murine nasal anti-influenza virus immunity. J. Virol., 1999; 65: 2146-2148.
- Renegar, K.B., Small, P.A. Jr.: Passive transfer of local immunity to influenza virus infection by IgA antibody. J. Immunol., 1991; 146: 1972-1978.
- Fukumu, H., Takeuchi, Y.: Vaccination against parainfluenza 1 virus (typus muris) infection in order to eradicate this virus in colonies of laboratory animals. J. Biol. Standard., 1975; 28: 477- 481.
- Bizhanov, G., Vyshniauskis, G.: A comparison of three methods for extracting IgY from the egg yolk of hens immunized with Sendai virus. Vet. Res. Commun., 2000; 24: 103-113.
- Haneberg, B., Kendall, D., Amerongen, H.M., Apter, F.M., Kraehenbuhl, J-P., Neutra, M.R.: Induction of specific immunoglobulin A in the small intestine, colon-rectum, and vagina, measured by a new method for collection of secretions from local mucosal surfaces. Infect. Immun., 1994; 62: 15-23.
- Lowry, O.H., Rosenbrough, N.Y., Farr, A.L., Randall, R.Y.: Protein measurement with the Folin phenol reagent. J. Biol. Chem., 1951; 193: 265-275.
- Kunisawa, J., Nakanishi, T., Takahashi, I., Okudaira, A., Tsutsumi, Y., Katayama, K., Nakagawa, S., Kiyono, H., Mayumi, T.: Sendai virus fusion protein mediates simultaneous induction of MHC class I/II-dependent mucosal and systemic immune responses via the nasopharyngeal-associated lymphoreticular tissue immune system. J. Immunol., 2001; 167: 1406-1412.
- Gupta, R.K., Relyveld, E.H., Lindblad, E.B., Bizzini, B., Ben- Efraim, S., Gupta, C.K.: Adjuvants – a balance between toxicity and adjuvanticity. Vaccine, 1993; 11: 293-306.
- Lycke, N., Holmgren, J.: Long-term cholera antitoxin memory in the gut can be triggered to antibody formation associated with protection within hours of an oral challenge immunization. Scand. J. Immunol., 1987; 25: 407-412.
- Tuomanen, E.: Piracy of adhesins: attachment of superinfecting pathogens to respiratory cilia by secreted adhesions of Bordetella pertussis. Infect. Immun., 1986; 54: 905-908.