Ufuk KAMBER, Mükremin Özkan ARSLAN, Güven GÜLBAZ, Gencay Taşkın TAŞÇI, Atila AKÇA

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Sarcocystis species are the most commonly encountered parasitic organisms in animals. Sarcocystis hominis (Sarcocystis bovihominis), the species found in beef cattle, is not visible macroscopically in muscle tissue. In recent years it has been established that, in addition to being a zoonotic parasite transmitted via the consumption of beef, the parasite produces toxins and causes food poisoning. Therefore, this study was undertaken in order to determine the extent to which Sarcocystis species are present in minced meat, meatballs, and fermented sausages produced from beef and marketed in Kars. A total of 150 samples, 50 of each product, were collected in the city center from butcher's shops, from businesses that produce such items, and from general stores. In order to detect sarcocysts in the samples, the cysts revealed by an enzymatic digestion test were examined microscopically. Genomic DNA extracted from the samples was stored at -20 °C for analysis by polymerase chain reaction (PCR). The microscopic analysis revealed that the parasite was present in 2 samples of minced meat (4%) and 1 sample of meatballs (2%). Analysis by PCR revealed that the parasite was present in 14 samples of minced meat (28%), 34 samples of meatballs (68%), and 1 sample of sausage (2%). These results indicate that the use of PCR in the routine analysis of meat preparations for the presence of Sarcocystis species should be increased, since the application of this technique would provide analysts with more conclusive results than examination by microscope.

Keywords:

Sarcocystis spp., microscopic examination, beef products, PCR,

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Fayer R. Sarcosystis spp. in human infections. Clin Microbiol Rev 2004; 17: 894 -902.
Şaki CE, Değer S, Özer E. Sarcosporidiosis in Turkey. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 2010; 21: 129 -134 (in Turkish with an English abstract).
İnal T. Kesim Hayvanı ve Et Muayenesi. 1st ed. İzmir, Turkey: Saray Kitabevleri; 1995 (in Turkish).
Lindsay DS, Blagburn BL, Braund KG. Sarcocystis spp. and sarcocystosis. Basic Applied Myology 1995; 5: 249 -254.
Sevinç F. Sığırlarda sarcosporidiosis. In: Özcel MA, editor. Veteriner Hekimliğinde Parazit Hastalıkları. 1st ed. İzmir, Turkey: Meta Basım Matb Hizm; 2013. pp. 75 -81 (in Turkish).
Kamata Y, Saito M, Irikura D, Yahata Y, Ohnishi T, Bessho T, Inui T, Watanabe M, Suqita-Konishi Y. A toxin isolated from Sarcocystis fayeri in raw horsemeat may be responsible for food poisoning. J Food Prot 2014; 77: 814 -819.
Abubakar S, Teoh BT, Sam SS, Chang LY, Johari J, Hooi PS, Lakhbeer-Singh HK, Italiano CM, Syed Omar SF, Wong KT et al. Outbreak of human infection with Sarcocystis nesbitti , Malaysia, 2012. Emerg Infect Dis 2013; 19: 1989 -1991.
Ghisleni G, Robba S, Germani O, Scanziani E. Identification and prevalence of Sarcocystis spp. cysts in bovine canned meat. Food Control 2006; 17: 69 694.
Khaniki GRJ, Kia EB. Detection of Sarcocystis cysts from meat supplied for hamburger in Iran by histological method. J Med Sci 2006; 6: 18 -21.
Bahari P, Salehi M, Seyedabadi M, Mohammadi A. Molecular identification of macroscopic and microscopic cysts of Sarcocystis in sheep in north Khorasan province, Iran. Int J Mol Cell Med 2014; 3: 51 -56.
Güçlü F, Aldemir OS, Güler L. Differential identification of cattle Sarcocystis spp. by random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Revue Med Vet 2004; 155: 440 -444.
Hajimohammadi B, Dehghani A, Ahmadi MM, Eslami G, Oryan A, Khamesipour A. Prevalence and species identification of Sarcocystis in raw hamburgers distributed in Yazd, Iran using PCR-RFLP. J Food Qual Hazards Control 2014; 1: 15- 20.
Arslan MÖ, Umur Ş. The prevalence of sarcocystiosis in cattle in Kars and Erzurum Province, Türkiye. Türkiye Parazitol Derg 1997; 21: 417-420 (in Turkish with an English abstract).
Emre Z, Alabay M, Çerçi H, Demirtaş H. Prevalence of Sarcocystis spp. in cattle in Elmadağ, Ankara, Turkey. Ankara Univ Vet Fak 1989; 36: 692 -703 (in Turkish with an English abstract).
Taşcı S, Toparlak M, Gül Y. Van mezbahasında kesilen sığırlarda sarcosporidiosis’in yayılışı. Ankara Univ Vet Fak 1989; 36: 254- 259 (article in Turkish with an English abstract).
Umur Ş, Arslan MÖ. The prevalence of helmint species in stray dogs in Kars district. Türkiye Parazitol Derg 1998; 22: 188 -193 (in Turkish with an English abstract).
Pritt B, Trainer T, Simmons-Arnold L, Evans M, Dunams D, Rosenthal BM. Detection of Sarcocystis parasites in retail beef: a regional survey combining histological and genetic detection methods. J Food Prot 2008; 71: 2144 -2147.
Savini G, Dunsmore JD, Robertson ID, Seneviratna P. The epidemiology of Sarcocystis spp. in cattle of Western Australia. Epidemiol Infect 1992; 108: 107 -113.
Woldemeskel M, Gebreab F. Prevalence of sarcocysts in livestock of northwest Ethiopia. Zentralbl Veterinarmed B 1996; 43: 55 -58.
Toparlak M. Isolation of sarcocystis cysts frorn bovine rnuscles by using rnodified trypsindigestion technique. Ankara Univ Vet Fak 1987; 34: 363 -367 (in Turkish with an English abstract).
Hiepe T, Jungman R. Veterinarmedizinische Protozoologie. In: Hiepe T, editor. Lehrbuch der Parasitologie Bd 2. 1st ed. Stuttgart, Germany: Gustav Fischer; 1983. pp. 156 -173 (in German).
Gjerde B. Molecular characterisation of Sarcocystis bovifelis , Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle ( Bos taurus ) and Sarcocystis sinensis from water buffaloes ( Bubalus bubalis ). Parasitol Res 2016; 115: 1473- 1492.
Maskar Ü, Özden M, Dikmen S. Çeşitli kasaplık hayvan türleri ile et müstehzarlarında sarcosporidi bakımından histolojik araştırma. Mikrobiyoloji Dergisi 1971; 24: 86 -106 (in Turkish).
Sancak YC, Akdemir C, Berktaş M. Van piyasasında tüketime sunulan et ve et ürünlerinde sarcosporidiosis’in yayılışı. Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Dergisi 1995; 2: 114- 116 (in Turkish).
Rahdar M, Salehi M. The prevalance of Sarcocystis infection in meat-production by using digestion method in Ahvaz, Iran. Jundishapur J Microbiol 2011; 4: 295 -300.
Meistro S, Peletto S, Pezzolato M, Varello K, Botta M, Richelmi G, Biglia C, Baioni E, Modesto P, Acutis P et al. Sarcocystis spp . prevalence in bovine minced meat: A histological and molecular study. Ital J Food Saf 2015; 4: 85 -87.
Al-Hyali NS, Aljawady MA, Mohammad-Fakhri MA. The influence of some physio-chemical properties of sarcotoxin in rats. J Anim Vet Adv 2010; 9: 302 -305.