Haematology of downer dairy cows with fatty liver

This study examined downer dairy cows with concurrent fatty liver of different degrees and evaluated the haematological values, the relationship and joint occurrence of fatty liver severity and haematology and compared the haematological values in the downer animals that finally died or cured. Blood and liver biopsy samples were obtained from 36 Holstein downer cows shortly after they became recumbent and before they were treated. Blood analysis included determination of haematocrit, haemoglobin, white blood, granular cell and lymphocytes count, and platelet number. Liver tissue was examined histologically and was classified according to severity of fatty liver. The majority of downer cows had fatty liver. Concerning outcome, 9 of the 36 cows were finally cured and 27 were confirmed dead by the deadline decided for cases' follow up, which was the 7th day-in-milk. There was a significant association between clinical outcome (cured or died) and severity of fatty liver, while none of the haematological parameters showed any significant difference between the cured vs. animals that died. The haematology of downer cows with concurrent fatty liver did not offer sound help in patient evaluation. There was no strong correlation between blood parameters and liver triglyceride content or other liver diagnostic parameters. Neither haematological parameter showed any significant difference between animals which finally either died or were cured.

Haematology of downer dairy cows with fatty liver

This study examined downer dairy cows with concurrent fatty liver of different degrees and evaluated the haematological values, the relationship and joint occurrence of fatty liver severity and haematology and compared the haematological values in the downer animals that finally died or cured. Blood and liver biopsy samples were obtained from 36 Holstein downer cows shortly after they became recumbent and before they were treated. Blood analysis included determination of haematocrit, haemoglobin, white blood, granular cell and lymphocytes count, and platelet number. Liver tissue was examined histologically and was classified according to severity of fatty liver. The majority of downer cows had fatty liver. Concerning outcome, 9 of the 36 cows were finally cured and 27 were confirmed dead by the deadline decided for cases' follow up, which was the 7th day-in-milk. There was a significant association between clinical outcome (cured or died) and severity of fatty liver, while none of the haematological parameters showed any significant difference between the cured vs. animals that died. The haematology of downer cows with concurrent fatty liver did not offer sound help in patient evaluation. There was no strong correlation between blood parameters and liver triglyceride content or other liver diagnostic parameters. Neither haematological parameter showed any significant difference between animals which finally either died or were cured.

___

  • Barrington, G.M.: Parturient paresis in cows. In Aiello, S. Ed. Th e Merck veterinary manual. 8th ed., Merck and Co. Inc., Whitehouse Station, New Jersey, USA. 1998; 739-741.
  • Gerloff , B.J.: Herd health. In: Radostits, O., Ed. Food Animal Production Medicine, 3rd ed., W.B. Saunders G, Philadelphia. 2001; 465.
  • Morrow, D.A., Hillman, D., Dade, A.W., Kitch, H.: Clinical investigation of a dairy herd with the fat cow syndrome. J. Am. Vet. Med. Assoc., 1979; 15: 161-167.
  • Rukkwamsuk, T., Kruip, T.A., Wensing, T.: Relationship between overfeeding and overconditioning in the dry period and the problems of high producing dairy cows during the postparturient period. Vet. Q., 1999; 21: 71-77.
  • Collins, R.A., Reid, I.M.: A correlated biochemical and stereological study of periparturient fatty liver in the dairy cow. Res. Vet. Sci., 1980; 28: 373-376.
  • Staufenbiel, R., Staufenbiel, B., Rossow, N., Klukas, H., Johannsen U.: Diagnostik der Leberverfettung bei der Milchkuh. Deut. Tierärzl. Woch., 1993; 100: 225-230. (article in German with an abstract in English)
  • Cebra, C.K., Garry, F.B., Getzy, D.M., Fettman, M.J.: Hepatic lipidosis in anorectic, lactating Holstein cattle: a retrospective study of serum biochemical abnormalities. J. Vet. Intern. Med., 1997; 4: 231-237.
  • Reid, I.M., Roberts, C.J.: Subclinical fatty liver in dairy cows, current research and future prospects. Ir. Vet. J., 1983; 37: 104- 110.
  • Gerloff , B.J., Herdt, T.H., Emery, R.S.: Relationship of hepatic lipidosis to health and performance in dairy cattle. J. Am. Vet. Med. Assoc., 1986; 188: 845-850.
  • Reid, I.M., Dew, A.M., Williams, L.A.: Haematology of subclinical fatty liver in dairy cows. Res. Vet. Sci., 1984; 37: 63- 65.
  • Folch, J., Lees, M., Stanley, G.H.S.: A simple method for the isolation and purifi cation of total lipid from animal tissues. J. Biochem., 1957; 226: 497-509.
  • Eggstein, M., Kuhlmann, E.: Triglycerides and glycerol: Determination aft er alkaline hydrolysis. In: Bergmeyer, H.U., Ed. Methods of Enzymatic Analysis, 2nd ed., Vol 3, Academic Press, New York. 1974; 1825-1831.
  • Mertens, M.: Liver biopsy -histologic and enzymhistochemistry evaluation of liver biopsies from cows with dislocatio abomasi sinistra [Leberbiopsie beim Rind-Histologische und enzymhistochemische Auswertung von Bioptaten aus Kühen mit dislocatio abomasi sinistra]. PhD Dissertation. Tierärzliche Hochschule, Hannover, Germany. 1992.
  • Divers, T.J., Peek, S.F.: Th e clinical examination, table 1-2. In: Divers, T.J., Peek, S.F., Eds. Diseases of dairy cattle. 2nd ed., Saunders Elsevier, .St Louis, Missouri. 2008: 14.
  • Stöber, M., Grunder, H.D.: Circulation. In: Rosenberger, G., Ed. Clinical examination of cattle (Translation of the second edition 1977). Verlag Paul Parey, Berlin and Hamburg. 1979: 128.
  • Ahmed, M.M.I.: Examination of diff erent diagnostic methods and signifi cance of fatty liver at clinically diseased dairy cows and its relation to hypophosphatemia. [Untersuchungen zur Diagnostik und Bedeutung der Fettleber bei klinisch kranken Milchkuhen unter besonderer Berucksichtigung moglicher Beziehungen zur Hypophosphatamie]. PhD Dissertation. Freien Universität Berlin, Berlin. 2004.
  • Weiss, D.J., Perman, V.: Assessment of the hematopoietic system in ruminants. Vet. Clin. N. Am. - Food Animal Practice, 1992; 8: 411-428.
  • Wentick, G.H., Rutten, V.P.M.G., Van den Ingh, Hoek, A., Müller, K.E., Wensing, T.: Impaired specifi c immunoreactivity in cows with hepatic lipidosis. Vet. Immunol. Immunop., 1997; 56: 77-83.
  • Zerbe, H., Schneider, N., Leibold, W., Wensing, T., Kruip, T.A.M., Schuberth, H.J.: Altered functional and immunophenotypical properties of neutrophilic granulocytes in post partum cows associated with fatty liver. Th eriogenology, 2000; 54: 771-786.
  • Padilla-Arenalles, S., Conctantino-Casas, F., Nunez-Ochoa, L., Doubek, J., Vega-Murguia, C., Bouda, J.: Coagulation tests and selected biochemical analytes in dairy cows with hepatic lipidosis. Acta Vet. Brno, 2007; 76: 541-546.
  • Jain, N.C.: Cattle: Normal hematology with comments on response to disease. In: Schalm O.W. Ed. Schalm’s Veterinary Hematology, 4th ed., Lea and Febiger, Philadelphia. 1986: 178- 207.
  • Cole, D.J., Roussel, A.J., Whitney, M.S.: Interpreting a bovine CBC: evaluating the leukon and acute phase proteins. Vet. Med-Us., 1997; 92: 470-478.
  • Franklin, S.T., Young, J.W., Nonnecke, B.J.: Eff ect of ketones, acetate, butyrate and glucose on bovine lymphocyte proliferation. J. Dairy Sci., 1991; 74: 2507-2514.
  • Whitney, M.S.: Hematology of food animals. In: Howard J.L. Ed. Current Veterinary Th erapy III, Food Animal Practice, WB Saunders, Philadelphia. 1993; 690-698.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yong-Jun LI, Hong AO, Gui-jin SUN, Li ZHANG

Haematology of downer dairy cows with fatty liver

Emmanouil KALAITZAKIS, Nikolaos PANOUSIS, Nikolaos ROUBIES

Influence of PGF2a on semen quality and libido in Holstein bulls

Reza MASOUMI, Armin TOWHIDI, Ardsher N. JAVAREMI, Habib NABIZADEH, Mehdi ZHANDI

Diet preference and breeding success in captive-bred Greater rheas (Rhea americana): a preliminary study

Gisela BAZZANO, Joaquín L. NAVARRO, Mónica B. MARTELLA

Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls

Teng-He MA, Ji-Feng LIU, Rui-Feng ZHAO, Hao JIANG, Li-Sheng DAI, Yu-Min ZHAO, Zhi-Hui ZHAO, Jia-Bao ZHANG

Alteration of gene expression in microvascular endothelial cells after exposure to foot-and-mouth disease virus 146S antigen

Tao ZHANG, Shuang ZHANG, Li-Fang TIAN, Ge HU, Zhan-Wei SUO, Wu CHEN, Hui-Qin DUAN, Xiang MU, Ke-Dao TENG

Aflatoxin M1 levels in retail yoghurt and ayran in Erzurum in Turkey

Meryem AYDEMİR ATASEVER, Mustafa ATASEVER, Korhan ÖZTURAN

Association between polymorphisms of CYP19, CYP21, and ER1 genes and milk production traits in Black-and-White cattle

Magdalena J&#;DRZEJCZAK, Wilhelm GRZESIAK, Iwona SZATKOWSKA

Influence of $PGF_{2alpha}$ on semen quality and libido in Holstein bulls

Ardsher N. JAVAREMI, Habib NABIZADEH, Mehdi ZHANDI, Armin TOWHIDI, Reza MASOUMI

Histological examination of testicular cell development and apoptosis in the ostrich chick

Lan WEI, Ke-mei PENG, Huazhen LIU, Hui SONG, Yan WANG, Lia TANG