Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta

Öz Thirty-five Anatolian water buffalo were selected from among a total of almost 7000 animals from 11 herds in the Kızılırmak Delta, which is known to be one of the most important areas for water buffalo farming in Turkey. The cleaved amplified polymorphic sequences (CAPS) method was used to identify single-nucleotide polymorphisms at 3 loci that are associated with milk production, namely PRL, CSN3, and PIT-1, which are associated with milk processing and milk yields, respectively. Sequence-tagged sites of PRL, CSN3, and PIT-1 were digested by restriction endonucleases RsaI, HindIII, and HinfI, respectively. Capillary electrophoretic analysis identified all samples as belonging to the GG and BB genotypes, which are undesirable genotypes for PRL and PIT-1 gene loci, but ideal for the CSN3 locus. Then the samples were sequenced by an ABI 3730 DNA Analyzer (Applied Biosystems). DNA sequencing of the amplified CSN3 fragment revealed one polymorphism at codon 135 (ThrACC > IleATC) in some samples. A homozygous structure was found for all three loci and the huge population loss between 1991 and 2008 suggests that a genetic bottleneck may have occurred in the population of Anatolian water buffalo in the Kızılırmak Delta.

Kaynak Göster

Bibtex @ { tbtkveterinary663788, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {43}, pages = {784 - 790}, doi = {}, title = {Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta}, key = {cite}, author = {Toparslan, Emine and Mercan, Levent} }
APA Toparslan, E , Mercan, L . (2019). Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta . Turkish Journal of Veterinary and Animal Sciences , 43 (6) , 784-790 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663788
MLA Toparslan, E , Mercan, L . "Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta" . Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 784-790 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663788>
Chicago Toparslan, E , Mercan, L . "Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta". Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 784-790
RIS TY - JOUR T1 - Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta AU - Emine Toparslan , Levent Mercan Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 784 EP - 790 VL - 43 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta %A Emine Toparslan , Levent Mercan %T Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta %D 2019 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 43 %N 6 %R %U
ISNAD Toparslan, Emine , Mercan, Levent . "Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta". Turkish Journal of Veterinary and Animal Sciences 43 / 6 (Aralık 2019): 784-790 .
AMA Toparslan E , Mercan L . Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 784-790.
Vancouver Toparslan E , Mercan L . Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 784-790.
IEEE E. Toparslan ve L. Mercan , "Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta", Turkish Journal of Veterinary and Animal Sciences, c. 43, sayı. 6, ss. 784-790, Ara. 2019

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