Genetic diversity of different avian strains of Pasteurella multocida recovered during four outbreaks in India
Pasteurella multocida
causes fowl cholera in avian species and type A is predominantly reported from outbreaks of fowl
cholera. Biochemical, serological, and molecular methods are employed for its diagnosis and typing. Utilizing rapid molecular tools like
repetitive extragenic palindromic (REP) PCR and enterobacterial repetitive intergenic consensus (ERIC) PCR,
P. multocida
isolates from
four outbreaks (three from Chennai and one from Ahmadabad, India) were characterized and typed to determine their relationships. A
total of 36 isolates were recovered from the outbreaks, including one isolate from a parakeet, which was also subjected to characterization
by conventional and molecular analysis. All of the isolates were found to be capsular type A based on PCR assay capsular typing. ERIC-
and REP-PCR showed differences in the banding patterns among different outbreak isolates, and also among the geographical regions.
Differences were also noticed among different host species, as the banding pattern in the ERIC- and REP-PCR differed; the analysis of
results also revealed the same. All of the isolates were found to be sensitive to enrofloxacin and cefotaxime among the antibiotics used in
the study. It was found that different strains might have been involved in the different outbreaks reported in the study. The results show
that molecular typing methods like ERIC- and REP-PCR are useful epidemiological tools for classifying the strains.
___
- Wang C, Wu Y, Xing X, Hu G, Dai J, He H. An outbreak of avian
cholera in wild waterfowl in Ordos Wetland, Inner Mongolia,
China. J Wildlife Dis 2009; 45: 1194-1197.
- Ozyildiz Z, Tel OY, Yilmaz R, Ozsoy SY, Keskin O. Pathological
and microbiological investigations of pneumonic pasteurellosis
in sheep. Kafkas Univ Vet Fak 2013; 19: 103-108.
- Rimler RB, Glisson JR. Fowl cholera. In: Calnek BW, Barnes
HJ, Beard CW, Mcdougald LR, Saif YM, editors. Diseases of
Poultry. 10th ed. Ames, IA, USA: Iowa State University Press;
1997. pp. 143-159.
- Shivachandra SB, Kumar AA, Gautam R, Saxena MK,
Chaudhuri P, Srivastava SK. Detection of multiple strains of
Pasteurella multocida
in fowl cholera outbreaks by polymerase
chain reaction-based typing. Avian Pathol 2005; 34: 456-462.
- Srinivasan P, Gopalakrishnamurthy TR, Mohan B, Saravanan
S. Occurrence of subacute fowl cholera in a broiler flock. Tamil
Nadu J Vet Animal Sci 2011; 7: 45-47.
- Carter GR. Studies on
Pasteurella multocida
. I. A
haemagglutination test for the identification of serological
types. Am J Vet Res 1955; 16: 481-484.
- Sellyei B, Varga Z, Ivanics E, Magyar T. Characterisation
and comparison of avian
Pasteurella multocida
strains by
conventional and ERIC-PCR assays. Acta Vet Hung 2008; 56:
429-440.
- Shivachandra SB, Kumar AA, Chaudhuri P. Molecular
characterization of avian strains of
Pasteurella multocida
serogroup-A:1 based on amplification of repetitive regions by
PCR. Comp Immunol Microbiol Inf Dis 2008; 31: 47-62.
- Varga Z, Volokhov DV, Stipkovits L, Thuma A, Sellyei B,
Magyar T. Characterization of
Pasteurella multocida
strains
isolated from geese. Vet Microbiol 2013; 163: 149-156.
- Mohamed MWA, Mageed MAAMA. Molecular analysis
of
Pasteurella multocida
strains isolated from fowl cholera
infection in backyard chickens. Asian Pac J Trop Biomed 2014;
4: 8-12.
- Singh R, Blackall PJ, Remington B, Turni C. Studies on the
presence and persistence of
Pasteurella
multocida
serovars and
genotypes in fowl cholera outbreaks. Avian Pathol 2013; 42:
581-585.
- Shirzad-Aski H, Tabatabaei M. Molecular characterization of
Pasteurella multocida
isolates obtained from poultry, ruminant,
cats and dogs using RAPD and REP-PCR analysis. Mol Biol
Res Commun 2016; 5: 123-132.
- Muhairwa AP, Christensen JP, Bisgaard M. Investigations on
the carrier rate of
Pasteurella multocida
in healthy commercial
poultry flocks and flocks affected by fowl cholera. Avian Pathol
2000; 29: 133-142.
- Zhang PJ, Fegan N, Fraser I, Duffy P, Gordon A, Ketterer PJ,
Shinwari W, Blackall PJ. Molecular epidemiology of two fowl
cholera outbreaks on a free-range layer farm. J Vet Diag Invest
2004; 16: 458-460.
- Bauer AW, Kirby WM, Sherris JC, Turck AM. Antibiotic
susceptibility test by a standardized single disc method. Am J
Clin Pathol 1966; 45: 493-496.
- Townsend KM, Frost AJ, Lee CW, Papadimitrious JM, Dawkins
HJ. Development of PCR assays for species and type specific
identification of
Pasteurella multocida
strains. J Clin Microbiol
1998; 36: 1096-1100.
- Townsend KM, Boyce JD, Chung JY, Frost AJ, Adler B. Genetic
organization of
Pasteurella multocida
cap loci and development
of a multiplex capsular PCR typing system. J Clin Microbiol
2001; 39: 924-929.
- Versalovic J, Koeuth T, Lupski JR. Distribution of repetitive
DNA sequences in eubacteria and application of fingerprinting
of bacterial genomes. Nuc Acids Res 1991; 19: 6823-6831.
- Hunter PR, Gaston MA. Numerical index of the discriminatory
ability of typing systems: an application of Simpson’s index of
diversity. J Clin Microbiol 1988; 26: 2465-2466.
- Chawak MM, Verma KC, Kataria JM, Kumar AA.
Characterization of indigenous isolates of avian
Pasteurella
multocida
. Indian J Comp Microbiol Immunol Infect Dis 2000;
21: 111-114.
- Sarangi LN, Thomas P, Gupta SK, Kumar S, Viswas KN,
Singh VP. Molecular Epidemiology of
Pasteurella multocida
circulating in India by multilocus sequence typing. Transbound
Emerg Dis 2016; 63: e286-e292.
- Mbuthia PG, Njagi LW, Nyaga PN, Bebora LC, Minga
U, Kamundia J, Olsen JE.
Pasteurella multocida
in scavenging
family chickens and ducks: carrier status, age susceptibility and
transmission between species. Avian Pathol 2008; 37: 51-57.
- Rhoades KR, Rimler RB. Fowl cholera. In: Adlam CF, Rutter
JM, editors. Pasteurella and Pasteurellosis. 1st ed. London, UK:
Academic Press; 1989. pp. 95-113.
- Kumar AA, Shivachandra SB, Biswas A, Singh VP, Singh VP,
Srivastava SK. Prevalent serotypes of
Pasteurella multocida
isolated from different animal and avian species in India. Vet
Res Commun 2004; 28: 657-667.
- Shivachandra SB, Kumar AA, Gautam R, Singh VP, Saxena
MK, Srivastava SK. Identification of avian strains of
Pasteurella
multocida
in India by conventional and PCR assays. Vet J 2006;
172: 561-564.
- Furian TQI, Borges KAI, Pilatti RMI, Almeida CI, Nascimento
VP do, Salle CTP, Moraes HL de S. Identification of the capsule
type of
Pasteurella multocida
isolates from cases of fowl cholera
by multiplex PCR and comparison with phenotypic methods.
Braz J Poult Sci 2014; 16: 31-36.
- Jabbari AR, Esmaelzadeh M, Moazeni Jula GR. Polymerase
chain reaction of
Pasteurella
multocida
capsules isolated in
Iran. Iran J Vet Res 2006; 7: 50-55.
- Rigobelo EC, Blackall PJ, Maluta RP, de Ávila FA. Identification
and antimicrobial susceptibility patterns of
Pasteurella
multocida
isolated from chickens and Japanese quails in Brazil.
Braz J Microbiol 2013; 44: 161-164.
- Snipes KP, Hirsh DC, Kasten RW, Carpenter TE, Hird DW,
McCapes RH. Homogeneity of characteristics of
Pasteurella
multocida
isolated from turkeys and wildlife in California,
1985-1988. Avian Dis 1990; 34: 315-320.
- Olson DL, Wilson MA. DNA fingerprint patterns of
Pasteurella
multocida
from the same turkey farm on the same and different
years. Avian Dis 2001; 45: 807-812.
- Blackall PJ, Pahoff JL, Marks D, Fegan N, Morrow CJ.
Characterisation of
Pasteurella
multocida
isolated from fowl
cholera outbreaks on turkey farms. Aus Vet J 1995; 72: 135-138.
- Dziva F, Muhairwa A, Bisgaard M, Christensen H. Diagnostic
and typing options for investigating diseases associated with
Pasteurella multocida
. Vet Microbiol 2008; 128: 1-2.
- Bisgaard M, Petersen A, Christensen H. Multilocus sequence
analysis of
Pasteurella multocida
demonstrates a type species
under development. Microbiol 2013; 159: 580-590.