First report of Brucella suis biovar 2 in outdoor reared pigs ( Sus scrofa domesticus ) in Serbia
The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals' owner discovered two aborted fetuses, thereafter presented for laboratory examinations. B. suis biovar 2 was isolated from both fetuses. Isolated strains were identified using both classical and molecular techniques, including genomic sequencing. Based on epizootiological data, we were unable to establish the source of infection.
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- World Organisation for Animal Health. Manual of Diagnostic
Tests and Vaccines for Terrestrial Animals. Paris, France: OIE;
2016.
- Corbel MJ, Brinley-Morgan WJ. Genus
Brucella
.
In: Krieg NR,
Holt JC, editors. Bergey’s Manual of Systematic Bacteriology.
Baltimore, MD, USA: Williams & Wilkins; 1984. pp. 377-388.
- Godfroid J, Nielsen K, Saegerman C. Diagnosis of brucellosis
in livestock and wildlife. Croat Med J 2010; 51: 296-305.
- Fretin D, Mori M, Czaplicki G, Quinet C, Maquet B, Godfroid
J. Saegerman C. Unexpected
Brucella suis
biovar 2 infection in
a dairy cow, Belgium. Emerg Infect Dis 2013; 19: 2053-2054.
- Ramamoorthy S, Woldemeskel M, Ligett A, Snider R, Cobb R,
Rajeev S.
Brucella suis
infection in dogs, Georgia, USA. Emerg
Infect Dis 2011; 12: 2386-2387.
- Cvetnic Z, Spicic S, Curi S, Jukic B, Lojkic M, Albert D,
Thiebaud M, Garin-Bastuji B. Isolation of
Brucella suis
biovar 3
from horses in Croatia. Vet Rec 2005; 156: 584-585.
- Szulowski K, Iwaniak W, Pilastek J, Truszczynski M,
Chrobocinska M. The ELISA for the examination of hare sera
for anti-
Brucella
antibodies. Comp Immunol Microb 1999; 22:
33-40.
- Hofer E, Reisp K, Revilla-Fernandez S, Plicka H, Romanek G,
Bago Z, Winter P, Köfer J. Isolation of
Francisella tularensis
and
Brucella suis
from red foxes (
Vulpes vulpes
). Tieraerztl
Umschau 2010; 65: 229-232.
- World Organisation for Animal Health. Manual of Diagnostic
Tests and Vaccines for Terrestrial Animals. Paris, France: OIE;
2009.
- Kutlu M, Cevahir N, Erdenliğ-Gürbilek S, Akalın Ş, Uçar M,
Sayın-Kutlu S. The first report of
Brucella suis
biovar 1 isolation
in human in Turkey. Journal of Infection and Public Health
2016; 9: 675-678.
- Megid J, Mathias LA, Robles CA.
Clinical manifestations of
Brucellosis in domestic animals and humans.
Open Veterinary
Science Journal 2010; 4: 119-126.
Godfroid J. Wildlife brucellosis: spillover or sustainable
infection? In: Brucellosis 2014 International Research
Conference: 67th Annual Brucellosis Research Meeting.
Berlin, Germany: BFR; 2014. pp. 205-208.
- European Food Safety Agency. Porcine brucellosis (
Brucella
suis
). Scientific Opinion of the Panel on Animal Health and
Welfare. EFSA Journal 2009; 1144: 1-112.
- Melzer F, Lohse R, Nieper H, Liebert M, Sachse K. A serological
study on brucellosis in wild boars in Germany. Eur J Wildlife
Res 2007; 53: 153-157.
- Cvetnic Z, Spicic S, Toncic J, Majnaric D, Benic M, Albert
M, Thiebaud M, Garin-Bastuji B.
Brucella suis
infection in
domestic pigs and wild boar in Croatia. Rev Sci Tech OIE 2009;
28: 1057-1067.
- Wu N, Abril C, Thomann A, Grosclaude E, Doher MG, Boujon
P, Ryser-Degiorgis MP. Risk factors for contacts between wild
boar and outdoor pigs in Switzerland and investigations on
potential
Brucella suis
spill-over. BMC Vet Res 2012; 8: 2-12.
- Garin-Bastuji B, Hars J, Calvez D, Thiebaud M, Artois M.
Brucellose du porc domestique et du sanglier sauvage due a
Brucella suis
biovar 2 en France. Rev Epid San Anim
2000; 38:
1-5 (in French).
- Spicic S, Benic M, Zdelar-Turk M, Duvnjak S, Karakas A, Kis T,
Sucec I, Cvetnic Z. Brucellosis in Turopolje pig breeding in the
2008-2011 period: an overview of laboratory diagnostics and
eradication system. Vet Arhiv 2016; 86: 243-252.
- Barlozzari G, Franco A, Macri G, Lorenetti S, Maggiori F,
Dottarelli S, Maurelli M, DiGiannatale E, Tittarelli M, Battisti
A, Gamberale F. First report of
Brucella suis
biovar 2 in a semi
free-range pig farm, Italy. Veter Ital Ser 2015; 51:
151-154.
- Alton GG, Jones LM, Angus RD, Verger JM. Techniques
for the Brucellosis Laboratory. 1st ed. National Institute of
Agricultural Research; 1988.
- Leal-Klevezas DS, Martinez-Vasquez IO, Lopez-Merino A,
Martinez-Soriano JP. Single-step PCR for detection of
Brucella
spp. from blood and milk of infected animals. J Clin Microbiol
1995; 33: 3087-3090.
- Bricker BJ, Halling SM. Differentiation of
Brucella abortus
bv.
1, 2, and 4,
Brucella melitensis
,
Brucella ovis
and
Brucella suis
bv. 1 by PCR. J Clin Microbiol 1994; 32: 2660-2666.
- Ewalt DR, Payeur JB, Rhyan JC, Geer PL.
Brucella suis
biovar
1 in naturally infected cattle: a bacteriological, serological, and
histological study. J Vet Diagn Invest 1997; 9: 417-420.
- Köppel C, Knopf L, Ryser MP, Miserez R, Thür B, Stärk KDC.
Serosurveillance for selected infectious disease agents in boars
(
Sus scrofa
) and outdoor pigs in Switzerland. Eur J Wildlife Res
2007; 53: 212-220.
- Pilo C, Tedde MT, Orru G, Addis G, Liciardi M.
Brucella suis
infection in domestic pigs in Sardinia (Italy). Epidemiol Infect
2014; 143: 1-21.
- Rogozarski D, Potkonjak A, Gagrcin M, Lako B, Plavsic B.
Isolation and identification of
Brucella suis
biotype 2 from
epididymal puncture performed on a boar affected with
brucellosis. Veterinarski Glasnik 2009; 63: 153-161 (article in
Serbian with an English abstract).
- Zutic J, Stanojevic S, Plavsic B, Vojinovic D. Prevalence and
significance of brucellosis in the Republic of Serbia. In: Book
of Abstracts of the Symposium Brucellosis in Croatia and
Neighbouring Countries. Zagreb, Croatia; 2013. pp. 1-2.
- Zutic J, Vojinovic D, Stanojevic S, Cvetojevic DJ, Plavsic B.
Seroepizootiological situation of brucellosis in Republic of
Serbia. In: Proceedings of the 2nd International Symposium of
Veterinary Medicine. Belgrade, Serbia; 2016. pp. 30-35.
- Duvnjak S, Racic I, Spicic S, Zdelar-Turk M, Reil I, Cvetnic
Z. Characterisation of
Brucella suis
isolates from Southeast
Europe by multi-locus variable-number tandem repeat
analysis. Vet Microbiol 2015; 180: 146-150.
- Kreizinger Z, Foster JT, Ronai Z, Sulyok KM, Wehmann E,
Gyuranecz M. Genetic relatedness of
Brucella suis
biovar
2 isolates from hares, wild boars and domestic pigs. Vet
Microbiol 2014; 172: 492-498.