First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences

Öz This report is the first molecular characterization of the facultative myiasis agent Eristalis tenax collected from cattle barns in Kayseri province of Central Anatolia, Turkey. A total of 10 adult fly specimens were included in the study. All flies were identified as female adults of E. tenax by morphologic characteristics under a stereomicroscope. The barcode region of the mitochondrial cytochrome oxidase 1 CO1 gene was amplified with PCR from the genomic DNA extracts of E. tenax specimens for molecular identification and phylogenetic analyses. There was no polymorphism among the CO1 sequences of all isolates leading to the detection of a single haplotype for E. tenax. Haplotype and nucleotide diversities in the CO1 data set comprised of the sequence of the detected haplotype and the published sequences of E. tenax from different countries in GenBank were determined as 0.775 and 0.002, respectively. The newly characterized haplotype in this study clustered with the haplotypes from Australia and Canada in a monophyletic clade and exhibited a 100.0% identity to the Australian E. tenax isolate JN991985 . Interspecific genetic differences between E. tenax and other Eristalis species were in the range of 5.7% to 7.9%.

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Kaynak Göster

Bibtex @ { tbtkveterinary724475, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {449 - 455}, doi = {10.3906/vet-1911-82}, title = {First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences}, key = {cite}, author = {Okur, Mübeccel and Yıldırım, Alparslan and İnci, Abdullah} }
APA Okur, M , Yıldırım, A , İnci, A . (2020). First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences . Turkish Journal of Veterinary and Animal Sciences , 44 (2) , 449-455 . DOI: 10.3906/vet-1911-82
MLA Okur, M , Yıldırım, A , İnci, A . "First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences" . Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 449-455 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/53838/724475>
Chicago Okur, M , Yıldırım, A , İnci, A . "First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences". Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 449-455
RIS TY - JOUR T1 - First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences AU - Mübeccel Okur , Alparslan Yıldırım , Abdullah İnci Y1 - 2020 PY - 2020 N1 - doi: 10.3906/vet-1911-82 DO - 10.3906/vet-1911-82 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 449 EP - 455 VL - 44 IS - 2 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/vet-1911-82 UR - https://doi.org/10.3906/vet-1911-82 Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences %A Mübeccel Okur , Alparslan Yıldırım , Abdullah İnci %T First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences %D 2020 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 44 %N 2 %R doi: 10.3906/vet-1911-82 %U 10.3906/vet-1911-82
ISNAD Okur, Mübeccel , Yıldırım, Alparslan , İnci, Abdullah . "First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences". Turkish Journal of Veterinary and Animal Sciences 44 / 2 (Mart 2020): 449-455 . https://doi.org/10.3906/vet-1911-82
AMA Okur M , Yıldırım A , İnci A . First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 449-455.
Vancouver Okur M , Yıldırım A , İnci A . First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 449-455.
IEEE M. Okur , A. Yıldırım ve A. İnci , "First molecular characterization of the facultative myiasis agent Eristalis tenax in Turkey using mitochondrial cytochrome c oxidase subunit I DNA barcoding sequences", Turkish Journal of Veterinary and Animal Sciences, c. 44, sayı. 2, ss. 449-455, Mar. 2020, doi:10.3906/vet-1911-82

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