First detection and molecular identification of Babesia gibsoni in two dogs from the Aydın Province of Turkey

Small (1-3 µm in diameter, ring, oval, or comma shaped) piroplasms suggestive of Babesia spp. were observed upon microscopic examination of Giemsa-stained peripheral blood smears from a 3.5-year-old American Pit Bull Terrier with clinical signs of pyrexia, weakness, mucous membrane pallor, and depression living in Aydın, Turkey, in February, 2009. To confirm the presence and to identify the species of Babesia spp., a polymerase chain reaction (PCR) technique was performed using primers amplifying a 670 bp fragment of the 18S rRNA gene of Babesia gibsoni and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the sequences available in GenBank using the nucleotide Basic Local Alignment Search Tool program. The results indicated a 99% similarity with sequences of Babesia gibsoni. A 2-year-old American Staffordshire Terrier with no signs of any diseases was also confirmed to be infected with B. gibsoni using the same methods. To the best of our knowledge, this is the first report demonstrating the presence of B. gibsoni in Aydın, Turkey.

First detection and molecular identification of Babesia gibsoni in two dogs from the Aydın Province of Turkey

Small (1-3 µm in diameter, ring, oval, or comma shaped) piroplasms suggestive of Babesia spp. were observed upon microscopic examination of Giemsa-stained peripheral blood smears from a 3.5-year-old American Pit Bull Terrier with clinical signs of pyrexia, weakness, mucous membrane pallor, and depression living in Aydın, Turkey, in February, 2009. To confirm the presence and to identify the species of Babesia spp., a polymerase chain reaction (PCR) technique was performed using primers amplifying a 670 bp fragment of the 18S rRNA gene of Babesia gibsoni and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the sequences available in GenBank using the nucleotide Basic Local Alignment Search Tool program. The results indicated a 99% similarity with sequences of Babesia gibsoni. A 2-year-old American Staffordshire Terrier with no signs of any diseases was also confirmed to be infected with B. gibsoni using the same methods. To the best of our knowledge, this is the first report demonstrating the presence of B. gibsoni in Aydın, Turkey.

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Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

A case of chondrosarcoma in a pheasant (Phasianus colchicus)

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The effects of propofol and a diazepam/alfentanil combination in dogs aged 10 years and above on heart rate, respiratory rate, pulse oximetry data, intraocular pressure, and body temperature

Özlem GÜZEL, Dilek OLĞUN ERDİKMEN, Esma YILDAR, Aslı EKİCİ, Murat ŞAROĞLU, Bülent EKİZ

Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle

Yingxia LIU, Jie CAO, Junjie ZHANG, Kai HUANG, Changming QI

Effect of extenders, postdilution intervals, and seasons on semen quality in dairy goats

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Evaluation of facial paralysis with electromyography (EMG) associated with hypothyroidism in a dog

Banu DOKUZEYLÜL, Ebru ERAVCI, Alper DEMİRUTKU, Yalçın DEVECİOĞLU, Mehmet Erman OR

Evaluation of signalment, clinical, and laboratory variables as prognostic indicators in dogs with acute abdominal syndrome

Galina SIMEONOVA, Dinko DINEV, Tzvetan CHAPRAZOV, Rumen ROYDEV

Metazoan parasites of fish species from Lake Sığırcı (Edirne, Turkey)

Hafize Sibel ÇOLAK

Endometritis in Simmental cows: incidence, causes, and therapy options

Robert ZOBEL

Changes in transferrin gene expression in sea bass (Dicentrarchus labrax) challenged with Vibrio anguillarum

Menekşe Didem ERCAN, Süheyla KARATAŞ, Emre TURGAY, Mustafa KOLUKIRIK, Orhan İNCE, Bahar İNCE

clinical case: Vitamin C-responsive dermatosis in a Holstein native cross bull calf

Amin ANOUSHEPOUR, Mehdi SAKHA, Pejman MORTAZAVI