Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic ESBL-producing E. coli isolates were identified, eight of them had only the blaTEM-1. Twenty-five ESBL-producing isolates were detected. This research is the first on the investigation and detection of ESBL-producing E. coli isolates from broilers in Turkey. In this study, 8.3% ESBL-producing E. coli were isolated from the cloacal samples of broilers collected from slaughterhouses in Turkey. CTX-M-15 (80%) was the most frequently isolated ESBL type. Using PFGE analysis, it was determined that these isolates had clonal similarity.

___

  • Smet A, Martel A, Persoons D, Dewulf J, Heyndrickx M, Catry B, Herman L, Haesebrouck F, Butaye P. Diversity of extended- spectrum β-lactamases and class C β-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Vet Mic 2008; 144: 505-510.
  • Olsen RH, Bisgaard M, Löhren U, Robineau B, Christensen H. Extended-spectrum β-lactamase-producing Escherichia coli isolated from poultry: a review of current problems, illustrated with some laboratory findings. Avian Pathol 2014; 43: 199-208.
  • Ewers C, Antao EM, Diehl I, Philipp HC, Wieler LH. Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Appl Environ Microbiol 2009; 75: 184-192.
  • Li L, Jiang ZG, Xia LN, Shen JZ, Dai L, Wang Y, Huang SY, Wu CM. Characterization of antimicrobial resistance and molecular determinants of beta-lactamase in Escherichia coli isolated from chickens in China during 1970–2007. Vet Microbiol 2010; 26: 505-510.
  • Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical Veterinary Microbiology. 2nd ed. Mosby Elsevier, China; 2013.
  • CLSI - Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Document M100-S23. Twenty-third Informational Supplement. Wayne, PA, USA: 2013.
  • Briñas L, Moreno MA, Zarazaga M, Porrero C, Sáenz Y, García M, Dominguez L, Torres C. Detection of CMY-2, CTX-M-14, and SHV-12 β-lactamases in Escherichia coli fecal-sample isolates from healthy chickens. Antimicrob Agents Chemother 2003; 47: 2056-2058.
  • Jouini A, Vinue L, Slama KB, Saenz Y, Klibi N, Hammami S, Boudabous A, Torres C. Characterization of CTX-M and SHV extended-spectrum β-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia. J Antimicrob Chemother 2007; 60: 1137-1141.
  • Sáenz Y, Briñas L, Domínguez E, Ruiz J, Zarazaga M, Vila J, Torres C. Mechanisms of resistance in multiple-antibiotic- resistant Escherichia coli strains of human, animal and food origins. Antimicrob Agents Chemother 2004; 48: 3996-4001.
  • Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-2239.
  • Randall LP, Clouting C, Horton RA, Goldham NG, Wu G, Clifton-Hadley FA, Davies R H, Teale CJ. Prevalence of Escherichia coli carrying extended-spectrum β-lactamases (CTX - M and TEM - 52) from broiler chickens and turkeys in Great Britain between 2006 and 2009. J Antimicrob Chemother 2011; 66: 86-95.
  • Girlich D, Poirel L, Carattoli A, Kempf I, Lartigue MF, Bertini A, Nordmann P. Extended-spectrum beta-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France. Appl Environ Microbiol 2007; 73: 4681-4685.
  • Costa D, Vinue L, Poeta P, Coelho AC, Matos M, Saenz Y, Somaldo S, Zarazaga M, Rodrigues J, Torres C. Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in fecal samples of broiler. Vet Mic 2009; 138: 339-344.
  • Yuan L, Liu JH, Hu GZ, Pan YS, Liu ZM, Mo J, Wei YJ. Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China. J Med Microbiol 2009; 58: 1449-1453.
  • Dierikx CM, van der Goot JA, Smith HE, Kant A, Mevius DJ. Presence of ESBL/AmpC-producing Escherichia coli in the broiler production pyramid: a descriptive study. PLoS ONE 2013; 8: e79005. doi: 10.1371/journal.pone.0079005.
  • Warren RE, Ensor VM, O’Neill P, Butler V, Taylor J, Nye K, Harvey M, Livermore DM, Woodford N, Hawkey PM. Imported chicken meat as a potential source of quinolone- resistant Escherichia coli producing extended-spectrum β-lactamases in the UK. Antimicrob Agents Chemother 2008; 61: 504-508.
  • Pitout JDD, Nordmann P, Laupland KB, Poirel L. Emergence of Enterobacteriacea producing extended-spectrum β-lactamases (ESBLs) in the community. J Antimicrob Chemother 2005; 56: 52-59.
  • Bortolaia V, Guardabassi L, Trevisani M, Bisgaard M, Venturi L, Bojesen AM. High diversity of extended-spectrum β-lactamases in Escherichia coli isolates from Italian broiler flocks. Antimicicrob Agents Chemother 2010; 54: 1623-1626.
  • Tong P, Sun Y, Ji X, Du X, Guo X, Liu J, Zhu L, Zhou B, Zhou W, Liu G et al. Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in Escherichia coli isolated from chickens. Foodborne Pathog Dis 2015; 12: 345- 352.
  • Maciuca IE, Williams NJ, Tuchilus C, Dorneanu O, Guguianu E, Carp-Carare C, Rimbu C, Timofte D. High prevalence of Escherichia coli -producing CTX-M-15 extended-spectrum beta-lactamases in poultry and human clinical isolates in Romania. Microb Drug Resist. 2015; 21: 651-662.
  • Gür D, Gülay Z, Arıkan Akan Ö, Aktaş Z, Bal Kayacan Ç, Çakıcı Ö, Eraç B, Gültekin M, Öğünç D, Söyletir G et al. Resistance to newer beta-lactams and related ESBL types in Gram-negative nosocomial isolates in Turkish hospitals: results of the multicentre Hitit study. Mikrobiyol Bul 2008; 42: 537-544.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Yu ZHANG, Yiyu TONG, Yang CHEN, Zhengyang HUANG, Zhen ZHU, Yang ZHANG, Qi XU, Guohong CHEN

Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

Alper KARAGÖZ, Şinasi AŞKAR, Zahide DİLİK, Buket YURTERİ, Nilgün ÜNAL

Accelerated Achilles tendon healing with interleukin-1 receptor antagonist protein in rabbits

Ozren SMOLEC, Marko PECIN, Mario KRESZINGER, Snjezana VUKOVIC, Marija LIPAR, Berislav RADISIC, Josip KOS

Effect of different processing methods of pigeon pea ( Cajanus cajan ) on growth performance, carcass traits, and blood biochemical and hematological parameters of broiler chickens

Muhammad ARIF, Abdur REHMAN, Muhammad SAEED, Mohamed Ezzat Abd EL-HACK, Mahmoud ALAGAWANY, Haider ABBAS, Muhammad Asif ARIAN, Sarfaraz Ali FAZLANI, Abbasi İmtiaz HUSSAIN, Tugay AYASAN

Ali Asghar SAKI, Fatemeh SAHEBI ALA, Pouya ZAMANI, Dariush ALIPOUR, Masoumeh ABBASINEZHAD

The cSNP scanning and expression analysis of the duck FTH1 gene

Zhen ZHU, Yu ZHANG, Yiyu TONG, Qi XU, Zhengyang HUANG, Yang CHEN, Yang ZHANG, Guohong CHEN

Influence of dietary vitamin D 3 supplementation on the color changes in longissimus lumborum, vastus lateralis, semitendinosus, and semimembranosus beef muscles

Urszula Karolina RAFALSKA

Effects of Nigella sativa and Hypericum perforatum on wound healing

Fatih Mehmet KANDEMİR, Ali Said DURMUŞ, Mehmet Cengiz HAN, Mustafa ÖZKARACA, Aydın SAĞLIYAN, Cihan GÜNAY, Selim ÇOMAKLI, Dicle ÖZTOPALAN FIRAT

Investigation of effects of fucoidan polysaccharides extracted from two species of Padina on the wound-healing process in the rat

Bahareh SHABANPOUR, Moazameh KORDJAZI, Ebrahim ZABIHI, Mohammad Ali FARAMARZI, Hassan AHMADI GAVLIGHI, Seyed Mohammad Amin FEGHHI, Seyed Abbas HOSSEINI

Japanese quail performance, intestinal microflora, and molecularresponses to screened wheat and multienzyme diet

Ali Asghar SAKI, Fatemeh SAHEBI ALA, Dariush ALIPOUR, Masoumeh ABBASINEZHAD, Pouya ZAMAN