Establishment and biological research of the Jining Grey goat fibroblast line

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes could be effectively expressed in the cells. This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.

Establishment and biological research of the Jining Grey goat fibroblast line

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes could be effectively expressed in the cells. This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.

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Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Urinary diversion via preputial urethrostomy with bilateral pubic-ischial osteotomy in a dog

Masaaki KATAYAMA, Yasuhiko OKAMUR, Hiroaki KAMISHINA, Yuji UZUKA

The effects of prestorage leukoreduction and storage duration on the in vitro quality of canine packed red blood cells

Elif Ergül EKİZ, Murat ARSLAN, İbrahim AKYAZI, Evren Eraslan UYGUR

Molecular and kinetic characterization of lactate dehydrogenase enzyme in the heart and breast muscle of Japanese quail (Coturnix japonica)

Ram Pratap SINGH, Kochiganti Venkata Hanumat SASTRY, Radha AGARWAL, Nitin Kumar PANDEY, Vishesh Kumar SAXENA, Jag MOHAN

Ultrasound imaging, biochemical blood analyses, and weight investigations of dissectible fat depots in New Zealand white rabbits

Penka YONKOVA, Anton RUSENOV, Dean KANAKOV, Dimitrinka ZAPRYANOVA, Ekaterina VACHKOVA, Ayşe SERBEST, Rosen DIMITROV, Dimitar KOSTOV

Prevention of predicted neonatal isoerythrolysis with jaundice foal agglutination test in a newborn foal

Aykut Göktürk ÜNER, Güney KAYA, Aydın OLTU

Effects of dietary inclusion of clinoptilolite in colostrum and milk on certain haematological parameters of calves

Konstantinos POURLIOTIS, Maria KARATZIA, Efterpi CHRISTAKI

Comparison of different lactation curve models in Sahiwal cows

Vilas DONGRE, Ravindar Singh GANDHI, Avtar SINGH

The effect of intrauterine cephapirin treatment after insemination on conception rate in repeat breeder dairy cows subjected to the progesterone-based Ovsynch protocol

Ahmet GÜMEN, Gülnaz Yilmazbaş MECİTOĞLU, Abdulkadir KESKİN, Ebru KARAKAYA, Ali ALKAN, Umut TAŞDEMİR, Hayrettin OKUT

First record of Mothocya melanosticta Schioedte and Meinert, 1884 (Isopoda: Cymothoidae) from Egyptian pinecone soldierfish with special reference to its infestation status

Ismail Saad ELSHAHAWY, Abdel-Razek Yousf DESOUKY

Reproductive performance and serum concentrations of progesterone of heat-stressed Holstein heifers subjected to timed artificial insemination and progesterone supplementation

Abelardo CORREA CALDERÓN, Juan Augusto HERNÁNDEZ RIVERA, Leonel AVENDAÑO REYES, Ulises MACIAS CRUZ, Raúl DIAZ MOLINA, Francisco Daniel ALVAREZ VALENZUELA, Arnoldo GONZALEZ-REYNA