Effects of Nigella sativa and Hypericum perforatum on wound healing
In this study, it was aimed to determine the influence of applying Nigella sativa (NS) and Hypericum perforatum (HP) on wound healing. A total of 42 female albino 2-month-old Wistar rats were randomly separated into 3 main groups. Each of these main groups was separated into two subgroups for 7- and 14-day postoperative follow-ups. A total of 84 excisional skin wounds, 5 mm in diameter, were created on the backs of the rats. Placebo cream (control group), 50% NS oil cream, and 50% HP oil cream were applied topically for the first, second, and third group, respectively. The wounds were checked daily. Seven rats from each group were euthanized on the 7th and 14th days after the operation. Skin samples taken from wound areas were examined histologically and biochemically. The measurements revealed that the wounds healed faster in the study groups than in the control group (P < 0.001). It was found that NS had more antioxidant properties than the other treatments, and HP increased wound healing via its positive effects on epithelialization and granulation. The clinical, biochemical, and histological findings showed that NS and HP increased the healing of skin wounds.
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- Durgun T, Durmuş AS, Kaplan M. Effects of local
diphenylhydantoin in wound healing in the clinical and
experimental studies. FÜ Sağlık Bilimleri Dergisi 1999; 13:
151-157 (in Turkish with English abstract).
- Han MC, Durmuş AS, Karabulut E, Yaman I. Effects of Turkish
propolis and silver sulfadiazine on burn wound healing in rats.
Revue Med Vet-Toulouse 2005; 156: 624-627.
- Durmuş AS, Han MC, Yaman İ. Comparative evaluation of
collagenase and silver sulfadiazine on burned wound healing in
rats. FÜ Sağlık Bilimleri Veteriner Dergisi 2009; 23: 135-139.
- Durmus AS, Yaman M, Can HN. Effects of extractum cepae,
heparin, allantoin gel and silver sulfadiazine on burn wound
healing: an experimental study in a rat model. Vet Med-Czech
2012; 57: 287-292.
- Fabricant DS, Farnsworth NR. The value of plants used in
traditional medicine for drug discovery. Environ Health Pers
2001; 109 (Suppl. 1): 69-75.
- Hosseinzadeh H, Parvardeh S, Asl MN, Sadeghnia HR, Ziaee
T. Effect of thymoquinone and Nigella sativa seeds oil on lipid
peroxidation level during global cerebral ischemia reperfusion
injury in rat hippocampus. Phytomedicine 2007; 14: 621-627.
- Yaman I, Durmuş AS, Çeribaşı S, Yaman M. Effects of
Nigella
sativa
and silver sulfadiazine on burn wound healing in rats. Vet
Med-Czech 2010; 55: 619-624.
- Alenzi FQ, Alsakran Altamimi MA, Kujan O, Tarakji B, Tamimi
W, Bagader O, Al-Shangiti A, Talohi AN, Alenezy AK, Al-Swailmi
F et al. Antioxidant properties of
Nigella sativa
. J Mol Genet Med
2013; 7: 77.
- Mansour MA, Nagi MN, El-Khatib AS, Al-Bekairi AM. Effects
of thymoquinone on antioxidant enzyme activities, lipid
peroxidation and DT-diaphorase in different tissues of mice: a
possible mechanism of action. Cell Biochem Funct 2002; 20: 143-
151.
- Peşin Suntar I, Küpeli Akkol E, Yılmazer D, Baykal T,
Kırmızıbekmez H, Alper M, Yeşilada E. Investigations on the
in vivo wound healing potential of Hypericum perforatum L. J
Ethnopharmacol 2010; 127: 468-477.
- Di Paola R, Mazzon E, Muia C, Crisafulli C, Genovese T, Di Bella
P, Esposito E, Menegazzi M, Meli R, Suzuki H et al. Protective
effect of
Hypericum perforatum
in zymosan-induced multiple
organ dysfunction syndrome: relationship to its inhibitory effect
on nitric oxide production and its peroxynitrite scavenging
activity. Nitric Oxide 2007; 16: 118-130.
- Paterniti I, Briguglio E, Mazzon E, Galuppo M, Oteri G, Cordasco
G, Cuzzocrea S. Effects of
Hypericum perforatum
, in a rodent
model of periodontitis. BMC Complem Altern M 2010; 10: 73.
- Placer ZA, Cushmanni LL, Johnson BC. Estimation of products of
lipid peroxidation (as malondialdehyde) in biochemical systems.
Anal Biochem 1966; 16: 359-364.
- Aebi H. Catalase. In: Bergmeyer HU, editor. Methods in
Enzymatic Analysis. 1st ed. New York, NY, USA: Academic Press;
1983. pp. 276-286.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein
measurement with the Folin phenol reagent. J Biol Chem 1951;
193: 265-275.
- Sedlak J, Lindsay RHC. Estimation of total protein bound and
nonprotein sulfhydryl groups in tissue with Ellmann’s reagent.
Anal Biochem 1968; 25: 192-205.
- Matkovics B, Szabo I, Varga IS. Determination of enzyme
activities in lipid peroxidation and glutathione pathways. Lab
Diagn 1988; 15: 248-249.
- Sun Y, Larry WO, Ying L. A simple method for clinical assay of
superoxide dismutase. Clin Chem 1988; 34: 497-500.
- Gal P, Kilik R, Mokry M, Vidinsky B, Vasilenko T, Mozes S,
Bobrov N, Tomori Z, Bober J, Lenhardt L. Simple method of
open skin wound healing model in corticosteroid-treated
and diabetic rats: standardization of semi-quantitative and
quantitative histological assessments. Vet Med-Czech 2008; 53:
652-659.
- Erpek S, Kilic N, Kozaci D, Dikiciglu E, Kavak T. Effects of
flunixin meglumine, diclofenac sodium and metamizole
sodium on experimental wound healing in rats. Revue Méd Vét
2006; 157: 185-192.
- Mukherjee PK, Verpoorte R, Suresh B. Evaluation of in-
vivo wound healing activity of
Hypericum patulum
(family:
Hypericaceae) leaf extract on different wound model in rats. J
Ethnopharmacol 2000; 70: 315-321.
- Akhoondinasab MR, Khodarahmi A, Akhoondinasab M, Saberi
M, Iranpour M. Assessing effect of three herbal medicines in
second and third degree burns in rats and comparison with
silver sulfadiazine ointment. Burns 2015; 41: 125-131.
- Daryabeigi R, Heidari M, Hosseini SA, Omranifar M.
Comparison of healing time of the 2 degree burn wounds
with two dressing methods of fundermol herbal ointment and
1% silver sulfadiazine cream. Iranian Journal of Nursing and
Midwifery Research 2010; 15: 97-101.
- Kıyan S, Uyanıkgil Y, Altuncı YA, Çavuşoğlu T, Çetin
Uyanıkgil EÖ, Karabey F. Investigation of acute effects of
Hypericum perforatum
(St. John’s Wort-Kantaron) treatment
in experimental thermal burns and comparison with silver
sulfadiazine treatment. Ulus Travma Acil Cer 2015; 21: 323-
336.
- Mabrouk GM, Moselhy SS, Zohny SF, Ali EM, Helal TE,
Amin AA, Khalifa AA. Inhibition of methylnitrosourea
(MNU) induced oxidative stress and carcinogenesis by orally
administered bee honey and
Nigella
grains in Sprague Dawely
rats. J Exp Clin Cancer Res 2002; 21: 341-346.
- Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser I, Sezen
H, Buyukfirat E, Hazar A. Thymoquinone protects end organs
from abdominal aorta ischemia/reperfusion injury in a rat
model. Braz J Cardiovasc Surg 2015; 30: 77-83.
- Guo S, DiPietro LA. Factors affecting wound healing. J Dent
Res 2010; 89: 219-229.
- Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial
activity of
Hypericum perforatum
L. J Ethnopharmacol 2010;
131: 511-521.
- Dikmen M, Öztürk Y, Sagratini G, Ricciutelli M, Vittori S,
Maggi F. Evaluation of the wound healing potentials of two
subspecies of
Hypericum perforatum
on cultured NIH3T3
fibroblasts. Phytother Res 2011; 25: 208-214.
- Samadi S, Khadivzadeh T, Emami A, Moosavi NS, Tafaghodi M,
Behnam HR. The effect of
Hypericum perforatum
on the wound
healing and scar of cesarean. J Altern Complem Med 2010; 16:
113-117.