Francıs Anand LALI, Kulangara ANILKUMAR, Thazhathuveetıl ARAVINDAKSHAN

20782

Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

Osteopontin gene OPN is located in the quantitative trait loci QTL for milk production traits in bovine chromosome 6. In the present study the impact of cytosine to thymine transition in the intron-4 of OPN gene g.8514C > T on milk production traits was analysed in the Holstein Friesian crossbred cattle of Kerala. Genomic DNA was isolated and a fragment of 290 bp enclosing the polymorphic site was amplified and genotyped by restriction fragment length polymorphism RFLP using endonuclease, BSeN1. The genetic variants were distributed according to Hardy-Weinberg equilibrium. The T allele was found to be the major one T/0.76, C/0.24 and the genotype frequencies were TT/0.60, CT/0.32, and CC/0.08. The amplicons of genotypes were sequenced by sangers dideoxy termination technique and confirmed the mutation. Association study using the General Linear Model-Analysis of Variance GLMANOVA considering marker, season of calving, parity and herd as fixed factors and dairy trait as dependent variable revealed that none of the yield traits 305 day milk yield, peak yield, fat yield, protein yield, solids not fat yield, lactose yield, daily milk yield or composition traits fat percent, protein percent, solids not fat percent and lactose percent of milk production analysed were significantly differed between CT and TT genotyped animals. The OPN gene polymorphism g.8514C > T can be suggested for marker assisted selection MAS for future breeding programmes for dairy cattle only after extensive association studies.
Keywords:

Osteopontin gene, milk production traits single nucleotide polymorphism, RFLP, Holstein Friesian crossbred cattle of Kerala,

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  • 1. Meuwissen THE, Hayes BJ, Goddard ME. Prediction of total genetic value using genome-wide dense marker maps. Genetics 2001; 157: 1819-1829.
  • 2. Blasco A, Toro MA. A short critical history of the application of genomics to animal breeding. Livestock Science 2014; 166: 4-9.
  • 3. Dekkers JCM. Commercial application of marker- and geneassisted selection in livestock: Strategies and lessons. Journal of Animal Science 2004; 82: 313-328.
  • 4. Schmid M, Bennewitz J. Invited review: Genome-wide association analysis for quantitative traits in livestock–a selective review of statistical models and experimental designs. Archives Animal Breeding 2017; 60: 335-346.
  • 5. Olsen HG, Lien S, Gautier M, Nilsen H, Roseth A et al. Mapping of a milk production quantitative trait locus to a 420-kb region on bovine chromosome 6. Genetics 2005; 169: 275-283.
  • 6. Sodek J, Ganss B, McKee MD. Osteopontin. Critical Reviews in Oral Biology and Medicine 2000; 11: 279-303.
  • 7. Schack L, Lange A, Kelsen J, Agnholt J, Christensen B et al. Considerable variation in the concentration of osteopontin in human milk, bovine milk, and infant formulas. Journal of Dairy Science 2009; 92: 5378-5385.
  • 8. Dudemaine PL, Thibault C, Alain K, Bissonnette N. Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. Animal Genetics 2014; 45: 629-640.
  • 9. Bissonnette N. Short communication: Genetic effect of variations in the osteopontin gene (SPP1) with lactation persistency in dairy cattle. Journal of Dairy Science 2018; 101: 456-461.
  • 10. Prokesch A, Smorlesi A, Perugini J, Manieri M, Ciarmela P et al. Molecular aspects of adipoepithelial trans-differentiation in mouse mammary gland. Stem Cells 2014; 32: 2756-2766.
  • 11. Lali FA, Anilkumar K. Crossbreeding of cattle population in India. Journal of Indian Veterinary Association 2019; 17: 16- 24.
  • 12. Lali FA, Bindu KA. Microsatellite BM1500 polymorphism and milk production traits in Vechur and crossbred cattle of Kerala. Veterinarski Arhiv 2011; 81: 35-42.
  • 13. The International Committee for Animal Recording. The Global Standard for Livestock Data Procedure 2, Section 2 ICAR Guidelines for Computing Accumulated Lactation Yield. Rome, Italy: ICAR; 2020.
  • 14. Joseph BA. Assessment and alleviation of environmental stress on productive performance of rabbits. PhD, Kerala Veterinary and Animal Sciences University, Pookode, India, 2011.
  • 15. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. New York, NY, USA: Cold Spring Harbor Laboratory Press; 2001.
  • 16. Leonard S, Khatib H, Schutzkus V, Chang YM, Maltecca C. Effects of the osteopontin gene variants on milk production traits in dairy cattle. Journal of Dairy Science 2005; 88: 4083- 4086.
  • 17. Falconer DS, Mackay TFC. Introduction to Quantitative Genetics. 4th ed. Essex, UK: Longmans Green; 1996.
  • 18. Khatib H, Zaitoun I, Wiebelhaus-Finger J, Chang YM, Rosa GJM. The effect of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations. Journal of Dairy Science 2007; 90: 2966-2970.
  • 19. Mousavizadeh SA, Salehi A, Aminafshar M, Sayyadnejad MB Nazemshirazi MH. Novel SNPs of the ABCG2 gene and their associations with milk production traits in Iranian Holstein bulls. Journal of Agricultural Science and Technology 2013; 15: 1145-1151.
  • 20. Boleckova J, Matejickova J, Stipkova M, Kyselova J, Barton L. The effect of five polymorphisms with milk production traits in Czech Fleckvieh cattle. Czech Journal of Animal Science 2012; 57: 45-53.
  • 21. Anggraeni A, Talib C, Asmarasari SA, Herawati T, Andreas E. Genetic polymorphisms of IGF1, GH, and OPN genes in crosses Peranakan Ongole cattle based on birth type in Central Java. Indonesian Journal of Animal and Veterinary Sciences 2017; 22: 165-172.
  • 22. Łuczak IK, Hanna K. Genetic polymorphisms of FAM13A1, OPN, LAP3, and HCAP-G genes in Jersey cattle. Turkish Journal of Veterinary and Animal Sciences 2013; 37: 631-635.
  • 23. White SN, Casas E, Allan MF, Keele JW, Snelling WM et al. Beef cattle evaluation of six DNA markers developed for milk production traits reveals an Osteopontin polymorphism is associated with post weaning growth. Journal of Animal Science 2007; 85: 1-10.
  • 24. Mello F, Cobuci JA, Martins MF. Effect of the polymorphism g.8514C>T in the Osteopontin gene (SPP1) with milk yield in the dairy cattle breed Girolando. Animal Genetics 2012; 34: 67- 69.
  • 25. Kułaj D, Pokorska J, Ochrem A, Dusza M, Makulska J. Effects of the c.8514C>T polymorphism in the osteopontin gene (OPN) on milk production, milk composition and disease susceptibility in Holstein-Friesian cattle. Italian Journal of Animal Science 2019; 18: 546-553.
  • 26. Salehi A, Khadijeh N, Mahdi A, Mohammad BS, Rohoallah S. The effect of bovine osteopontin (OPN) gene with milk production traits in Iranian Holstein bulls. Iranian Journal of Biotechnology 2015; 13: 1092-1097.
  • 27. Pasandideh M, Mohammadabadi MR, Esmailizadeh AK, Tarang A. Effect of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle. Czech Journal of Animal Science 2015; 60: 97-104.
  • 28. Lacorte G, Machado M, Martinez M, Campos A, Maciel R et al. DGAT1 K232A polymorphism in Brazilian cattle breeds. Genetics and Molecular Research 2006; 5: 475-482.
  • 29. Braunschweig M, Hagger C, Stranzinger G, Puhan Z. Associations between casein haplotypes and milk production traits of Swiss Brown cattle. Journal of Dairy Science 2000; 83: 1387-1395.
  • 30. Boettcher P, Caroli A, Stella A, Chessa S, Budelli E et al. Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle. Journal of Dairy Science 2004; 87: 4311-4317.
  • 31. Suchit K, Anupama M, Sunil K, Kumar YA, Kumar NA. Role of Osteopontin in bovines and their association with different traits: A review. International Journal of Genetics 2018; 10: 370-372.
  • 32. Wang G, Vander Walt JM, Mayhew G, Li YJ, Zuchner S et al. Variation in the miRNA-433 binding site of FGF20 confers risk for Parkinson disease by overexpression of α-synuclein. American Journal of Human Genetics 2008; 82: 283-289.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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