Development of an Enzyme Immunoassay for the Determination of Testosterone
In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be
Anahtar Kelimeler:
Testosterone, enzyme immunoassay
Development of an Enzyme Immunoassay for the Determination of Testosterone
In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be
Keywords:
Testosterone, enzyme immunoassay,
- ISSN: 1300-0128
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
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