Determination of the survival levels of acid-adapted Escherichia coli O157:H7in sucuk (Turkish-type fermented sausage)

This study aimed to determine the time taken for Escherichia coli O157:H7 to adapt to different types of acids and the effect of acid adaptation on the survival level of E. coli O157:H7 in sucuk (Turkish-type fermented sausage). E. coli O157:H7 cells were adapted to acids in a tryptic soy broth (TSB) set to pH 5.5 for 1-, 2-, 3-, and 4-h periods using hydrochloric, acetic, lactic, and citric acid. The acid tolerance of acid-adapted and nontreated cells was determined in TSB of pH 3.5 and then acid-adapted and nontreated E. coli O157:H7 (105 CFU/g) was added to sucuk. In the adaptation trial in which acetic acid was used, the highest acid resistance was seen in the 3-h adaptation samples, and in the lactic acid trials, the highest acid resistance was seen in the 3- and 4-h adaptation samples. In the adaptation trials with hydrochloric acid, it was observed that the duration of the adaptation period did not increase the resistance. It was determined that the survival rate of E. coli O157:H7 in sucuk was significantly increased through acid adaptation. As a result, it was understood that acid-adapted E. coli O157:H7 can maintain its viability in sucuk for at least 15 days.

Anahtar Kelimeler:

Escherichia coli O157:H7, acid adaptation, acid tolerance, Turkish-type fermented sausage, sucuk

Determination of the survival levels of acid-adapted Escherichia coli O157:H7in sucuk (Turkish-type fermented sausage)

Keywords:

Escherichia coli O157:H7, acid adaptation, acid tolerance, Turkish-type fermented sausage, sucuk,

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Parry-Hanson AA, Jooste PJ, Buys EM. Relative gene expression in acid-adapted Escherichia coli O157:H7 during lactoperoxidase and lactic acid challenge in Tryptone Soy Broth. Microbiol Res 2010; 165: 546–556.
Rekow CL, Brashears MM, Brooks JC, Loneragan GH, Gragg SE, Miller MF. Implementation of targeted interventions to control Escherichia coli O157:H7 in a commercial abattoir. Meat Sci 2011; 87: 361–365.
Yoon Y, Mukherjee A, Belk KE, Scanga JA, Smith GC, Sofos JN. Effect of tenderizers combined with organic acids on Escherichia coli O157:H7 thermal resistance in non-intact beef. Int J Food Microbiol 2009; 133: 78–85.
Sofos JN, Geornaras I. Overview of current meat hygiene and safety risks and summary of recent studies on biofilms, and control of Escherichia coli O157:H7 in nonintact, and Listeria monocytogenes in ready-to-eat, meat products. Meat Sci 2010; 86: 2–14.
Weagant SD, Bryant JL, Bark DH. Survival of Escherichia coli O157 H7 in mayonnaise and mayonnaise-based sauces at room and refrigerated temperatures. J Food Protect 1994; 57: 629–631.
Ryu JH, Beuchat LR. Influence of acid tolerance responses on survival, growth, and thermal cross-protection of Escherichia coli O157:H7 in acidified media and fruit juices. Int J Food Microbiol 1998; 45: 185–193.
Leyer GJ, Wang L, Johnson EA. Acid adaptation of Escherichia coli O157:H7 increases survival in acidic foods. Appl Environ Microbiol 1995; 3752–3755.
Castanie-Comet Cornet MP, Cam K, Bastiat B, Cros A, Bordes P, Gutierrez C. Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE. Nucleic Acids Res 2010; 38: 3546–3554.
Alegre I, Abadias M, Anguera M, Usall, J, Vinas. I. Fate of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches under different storage conditions. Food Microbiol 2010; 27: 862–868.
Krin E, Danchin A, Soutourina O. Decrypting the H-NS- dependent regulatory cascade of acid stress resistance in Escherichia coli. BMC Microbiol 2010; 10: 273.
Singh M, Mullins HR, Simpson SM, Dickson JS. Effect of acid adaptation on thermal tolerance of Escherichia coli O157:H7 and Salmonella enterica in meat serum. J Food Safety 2010; 30: 111–123.
Senouci-Rezkallah K, Schmitt P, Jobin MP. Amino acids improve acid tolerance and internal pH maintenance in Bacillus cereus ATCC14579 strain. Food Microbiol 2011; 28: 364–372.
Dlamini BC, Buys EM. Adaptation of Escherichia coli O157:H7 to acid in traditional and commercial goat milk amasi. Food Microbiol 2009; 26: 58–64.
Toldra F, Reig M. Sausages. In: Hui YH, Chandan C, Clark S, editors. Handbook of Food Products Manufacturing: Health, Meat, Milk, Poultry, Seafood, and Vegetables. Hoboken, NJ, USA: Wiley & Sons; 2007. pp. 251–264.
Stolzenbach S, Lindahl G, Lundstrom K, Chen G, Byrne DV. Perceptual masking of boar taint in Swedish fermented sausages. Meat Sci 2009; 81: 580–588.
Ravyts F, Sten L, Goemaere O, Paelinck H, Vuyst, L, Leroy F. The application of staphylococci with flavour-generating potential is affected by acidification in fermented dry sausages. Food Microbiol 2010; 27: 945–954.
Aro JM, Nyam-Osor P, Tsuji K, Shimad K, Fukushima M, Sekikawa M. The effect of starter cultures on proteolytic changes and amino acid content in fermented sausages. Food Chem 2010; 119: 279–285.
Apaydin G, Ceylan ZK, Kaya M. The behavior of E. coli O157:H7 in sucuk. J Food Process Pres 2009; 33: 827–836.
Cheng HY, Yu RC, Chou CC. Increased acid tolerance of Escherichia coli O157:H7 as affected by acid adaptation time and conditions of acid challenge. Food Res Int 2003; 36: 49–56.
Soyer A, Ertas AH, Uzumcuoglu U. Effect of processing conditions on the quality of naturally fermented Turkish sausages (sucuks). Meat Sci 2005; 69: 135–141.
Harrigan WF. Laboratory Methods in Food Microbiology. 3rd ed. San Diego, CA, USA: Academic Press; 1998.
AOAC. Official Methods of Analysis. Washington, DC, USA: Association of Official Analytical Chemists; 2000.
Tosun H, Aktuğ Gönül Ş. The effect of acid adaptation conditions on acid tolerance response of Escherichia coli O157:H7. Turk J Biol 2005; 29: 197–202.
Öztürk F. Determination of the survival of Escherichia coli O157:H7 and Listeria monocytogenes adapted to acid and salt stress conditions in Turkish sucuk. PhD, Ankara University, Ankara, Turkey, 2010.
Buchanan RL, Edelson RL. pH-dependent stationary-phase acid resistance response of enterohaemorrhagic Escherichia coli in the presence of various acidulants. J Food Protect 1999; 62: 211–218.
Ryu JH, Deng Y, Beuchat LR. Behavior of acid-adapted and unadapted Escherichia coli O157:H7 when exposed to reduced pH achieved with various organic acids. J Food Protect 1999; 62: 451–455.
Goodson M, Rowbury RJ. Habituation to normally lethal acidity by prior growth of Escherichia coli at a sub-lethal acid pH value. Lett Appl Microbiol 1989; 8: 77–79.
Riordan DCR, Duffy G, Sheridan JJ, Whiting RC, Blair JS, McDowell DA. Effects of acid adaptation, product pH, and heating on survival of Escherichia coli O157:H7 in pepperoni. Appl Environ Microbiol 2000; 66: 1726–1729.