Şükrü KIRKAN, Ergün Ö. GÖKSOY, Osman KAYA

Detection of Listeria monocytogenes by using PCR in Helix pomatia

The detection of Listeria monocytogenes in Helix pomatia, both raw and cooked is presented here. Polymerase chain reaction (PCR) is a rapid procedure with both sensitivity and specificity for quick detection and identification of Listeria monocytogenes from raw and cooked Helix pomatia. A total of 30 bags (10 g each) of H. pomatia samples were investigated for the Listeria monocytogenes inlB gene with the PCR method. PCR amplification products demonstrated that 18 of the 30 samples showed positive reactions to Listeria monocytogenes in the PCR. All PCR positive samples showed specific amplification of the 343 bp fragment for Listeria monocytogenes.

Anahtar Kelimeler:

Listeria monocytogenes, Helix pomatia, PCR

Detection of Listeria monocytogenes by using PCR in Helix pomatia

Keywords:

Listeria monocytogenes, Helix pomatia, PCR,

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Rocourt, J.: Risk factors for listeriosis. Food Cont., 1996; 7: 195–202.
Ryser, E.T.: Foodborne listeriosis, In: E.T. Ryser and E.H. Marth (eds.) Listeria, Listeriosis, and Food Safety, 2nd Ed., Marcel Dekker Inc., New York, 1999: pp. 299–358.
Temelli, S., Dokuzlu, C., fien, M.K.C.: Determination of microbiological contamination sources during frozen snail meat processing stages. Food Cont., 2006; 17: 22-29.
Seeliger, H.P.R.: Listeriosis-history and actual developments. Infection, 1988; 16: 80-84.
Fleming, D.W., Cochi, S,L., MacDonald, K.L., Brondum, J., Hayes, P.S., Plikaytis, B.D., Holmes, M.B., Audurier, A., Broome, C.V., Reingold, A.L.: Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N. Engl. J. Med., 1985; 312: 404–407.
Linnan, M.J., Mascola, L., Lou, X.D., Goulet, V., May, S., Salminen, C., Hird, D.W., Yonekura, M.L., Hayes, P., Weaver, R., Audurier, A., Plikaytis, B.D., Fannin, S.L., Kleks, A., Broome, C.V.: Epidemic listeriosis associated with Mexican-style cheese. N. Engl. J. Med., 1988; 319: 823–828.
Farber, J.M.: Thermal resistance of Listeria monocytogenes in foods. Int. J. Food Microbiol., 1989; 8: 285-291.
Johnson, J., Doyle, M., Cassens, R.: Listeria monocytogenes and other Listeria spp. in meat and meat products. A review. J. Food Prot., 1990; 53: 81–91.
Klein, P.G., Juneja, V.K.: Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR. Appl. Environ. Microbiol., 1997; 63: 4441-4448.
EN ISO 11290-1: Microbiology of food and animal feeding stuffs––horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: detection method. Geneva: International Organization for Standardization, 1996.
ISO 10560: Milk and milk products–detection of Listeria monocytogenes. Geneva: International Organization for Standardization, 1999.
Olsen, J.E.: DNA-based methods for detection of foodborne bacterial pathogens. Food Res. Int., 2000; 33: 257–266.
Saiki, R.K., Scharf, S., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, H.A., Arnheim, N.: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 1985; 230: 1350–1354.
Erlich, H.A., Gelfand, D., Sninsky, J.J.: Recent advances in the polymerase chain reaction. Science, 1991; 252: 1643–1651.
Fitter, S., Heuzenroeder, M., Thomas, C.J.: A combined PCR and selective enrichment method for rapid detection of Listeria monocytogenes. J. Appl. Bacteriol., 1992; 73: 53-59.
Kaclíková, E., Pangallo, D., Drahovská, H., Oravcová, K., Kuchta, T.: Detection of Listeria monocytogenes in food, equivalent to EN ISO 11290-1 or ISO 10560, by a three-days polymerase chain reaction-based method. Food Cont., 2003; 14: 175-179.
Pangallo, D., Kaclíková, E., Kuchta, T., Drahovská, H.: Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene. New Microbiol., 2001; 24: 333–339.
Fuchs, R.S., Nicolaides, L.: Incidence of Listeria in hot and cold smoked fish. Lett. Appl. Microbiol., 1994; 19: 394– 396.
Huss, H.H., Reilly, A., Embarek, P.K. B.: Prevention and control of hazards in seafood. Food Cont., 2000; 11: 149–156.
Johnson, J.L., Lattuada, C.P.: Comparison of nucleic acid hybridization assays and biochemical characterization tests for the confirmation of Listeria monocytogenes. J. Food Prot., 1993; 56: 834–840.
Shank, F.R., Eliot, E.L., Wachsmuth, I.K., Losikoff M.E.: U.S. position on Listeria monocytogenes in foods. Food Cont., 1996; 7: 229–234.