Fahd ALSOBAYIL, Madeh SADAN, Abd Al-azeez AL-SHOSHAN

Comparison between the anaesthetic effects of xylazine–ketamine and diazepam–ketamine: physiological and blood parameters in young hamadryas baboons ( Papio hamadryas )

The objective of this study was to evaluate the anaesthetic effects on young hamadryas baboons (Papio hamadryas) of xylazine-ketamine (XK) compared to diazepam-ketamine (DK). Six healthy young male hamadryas baboons were first premedicated with xylazine HCl (0.5 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). After a 10-day washout period, the hamadryas baboons were premedicated with diazepam (1 mg/kg, IM) and anaesthetised 20 min later with ketamine (10 mg/kg, IM). The onset, duration, and the depth of anaesthesia were determined by recording palpebral, corneal, and jaw reflexes. The results showed a significant decrease in heart rate and rectal temperature after XK injection, while a significant reduction in respiratory rate was seen when using the DK protocol. A highly significant increase in the levels of glucose was observed with the XK regimen. Blood pressure decreased when using both anaesthetic regimens, but this reduction did not reach significant levels. The quality of recovery was better when using XK compared to DK. In conclusion, major complications including bradycardia, hypothermia, and hyperglycaemia should be considered with a combination of XK. However, no complications other than bradypnea and hypercapnia should be expected when immobilising young hamadryas baboons with DK. No significant difference was observed in CBC, electrolytes, or lactate level between the anaesthesia protocols.

Keywords:

Xylazine, diazepam ketamine, hamadryas baboons, anaesthesia,

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Asgharian B, Price O, McClellan G, Corley R, Einstein D, Jacob R, Harkema J, Carey S, Schelegle E, Hyde D et al. Development of a rhesus hamadryas baboon lung geometry model and application to particle deposition in comparison to humans. Inhal Toxicol 2012; 24: 869-899.
Barr CS, Schwandt ML, Newman TK, Higley JD. The use of adolescent nonhuman primates to model human alcohol intake: neurobiological, genetic, and psychological variables. Ann NY Acad Sci 2004; 1021: 221-233.
Voorhies TM, Ehrlich ME, Duffy TE, Carol K, Petito CK, Plum F. Acute hyperammonemia in the young primate: physiologic and neuropathologic correlates. Pediatr Res 1983; 17: 970-975.
Hyvärinen J, Hyvärinen L, Linnankoski I. Modification of parietal association cortex and functional blindness after binocular deprivation in young hamadryas baboons. Exp Brain Res 1981; 42: 1-8.
Bernacky BJ, Gibson SV, Keeling ME, Abee CR. Nonhuman Primates. In: Fox JG, Anderson LC, Loew FM, Quimby FW, editors. Laboratory Animal Medicine. 2nd ed. Orlando, FL, USA: Academic Press; 2002. pp. 676-891.
Carlsson HE, Schapiro SJ, Farah I, Hau J. Use of primates in research: a global overview. Am J Primatol 2004; 63: 225-237.
Rogers J, Hixson JE. Baboons as an animal model for genetic studies of common human disease. Am J Hum Genet 1997; 61: 489-493.
Lauerman WC, Platenberg RC, Cain JE, Vincent FX. Age related disk degeneration: preliminary report of a naturally occurring baboon model. J Spinal Disord 1992; 5: 170-174.
Green CJ, Knight J, Precious S, Simpkin S. Ketamine alone and combined with diazepam or xylazine in laboratory animals: a 10 year experience. Lab Anim 1981; 15: 163-170.
Sainsbury AW, Eaton BD, Cooper JE. Restraint and anaesthesia of primates. Vet Rec 1989; 125: 640-643.
Jacobs B, Harris GC, Allada V, Chugani HT, Pollack DB, Raleigh MJ. Midazolam as an effective intravenous adjuvant to prolonged ketamine sedation in young rhesus (Macaca mulatta) and vervet (Cercopithecus aethiops sabaeus) hamadryas baboons: a preliminary report. Am J Primatol 1993; 29: 291-298.
Nolosco TP, Rao GD, William BJ, Prathaban S. Clinical evaluation of diazepam-ketamine anaesthetic regimen in Macaca radiata (bonnet macaque). Tamilnadu J Vet Anim Sci (TNJVAS) 2009; 5: 56-58.
Vnuk D, Musulin, A, Kreszinger M, Pecin M, Bata I, Zubcic D, Lemo. Balanced anaesthesia in the Capuchin hamadryas baboon ( Cebus capucinus ) – a case report. Vet Arh 2009; 79: 421-428.
Popilskis SJ, Kohn DF. Anaesthesia and analgesia in nonhuman primates. In: Kohn DF, Wixson SK, White WJ, Benson GJ, editors. Anaesthesia and Analgesia in Laboratory Animals. San Diego, CA, USA: Academic Press; 1997. pp. 233-255.
April M, Tabor E, Gerety RJ. Combination of ketamine and xylazine for effective anaesthesia of juvenile chimpanzees ( Pan troglodytes ). Lab Anim 1982; 16: 116-117.
Woolfson MW, Foran JA, Freedman HM, Moore PA, Shulman LB, Schnitman PA. Immobilization of baboons ( Papio anubus ) using ketamine and diazepam. Lab Anim Sci 1980; 30: 902- 904.
Naccarato EF, Hunter WS. Anaesthesia effects of various ratios of ketamine and xylazine in rhesus hamadryas baboons ( Macaca mulatta ). Lab Anim 1979; 13: 317-319.
Banknieder AR, Phillips JM, Jackson KT, Vinal SJ. Comparison of ketamine with the combination of ketamine and xylazine for effective anaesthesia in the rhesus hamadryas baboons ( Macaca mulatta ). Lab Anim Sci 1978; 28: 742-745.
White GL, Cummings JF. A comparison of ketamine and ketamine-xylazine in the baboon. Vet Med Small Anim Clin 1979; 74: 392-394.
Moore GT. The breeding and utilization of baboons for biomedical research. Lab Anim Sci 1975; 25: 798-801.
Clergue F, Desmonts JM, Duvaldestin P, Delavault E, Saumon G. Depression of respiratory drive by diazepam as premedication. Br J Anaesth 1981; 53: 1059-1063.
Forster A, Gardaz JP, Suter P, Gemperle M. Respiratory depression by midazolam and diazepam. Anaesth 1980; 53: 494-497.
Jordan C, Lehane JR, Jones JG. Respiratory depression following diazepam: reversal with high-dose naloxone. Anaesth 1980; 53: 293-298.
Stewart RC. Respiratory depression with diazepam. Potential complications and contraindications. Anesth Prog 1978; 117- 118.
Norris E, Marzouk O, Nunn A, McIntyre J, Choonara I. Respiratory depression in children receiving diazepam for acute seizures: a prospective study. Dev Med Child Neurol 1999; 41: 340-343.
Appleton R, Sweeney A, Choonara I, Robson J, Molyneux E. Lorazepam versus diazepam in the acute treatment of epileptic seizures and status epilepticus. Dev Med Child Neurol 1995; 37: 683-688.
Leppik IE, Derivan AT, Homan RW, Walker J, Ramsey RE, Patrick B. Double-blind study of lorazepam and diazepam in status epilepticus. J Am Med Assoc 1983; 249: 1452-1454.
Reutlinger RA, Karl AA, Vinal SI, Nieser MJ. Effects of ketamine HCl-xylazine HC1 combination on cardiovascular and pulmonary values of the rhesus macaque (Macaca mulatta ). Am J Res 1980; 41: 1453-1457.
Sumitra M, Manikandan P, Rao KV, Nayeem M, Manohar BM, Puvanakrishnan R. Cardiorespiratory effects of diazepam– ketamine, xylazine–ketamine and thiopentone anaesthesia in male Wistar rats: a comparative analysis. Life Sci J 2004; 75: 1887-1896.
Kul M, Koc Y, Alkand F, Ogurtan Z. The effects of xylazine- ketamine and diazepam-ketamine on arterial blood pressure and blood gases in dogs. J Vet Res 2000; 4: 123-132.
Moens Y, Fargetton X. A comparative study of medetomidine/ ketamine and xylazine/ketamine anaesthesia in dogs. Vet Rec 1990; 127: 567-571.
Purohit RC, Mysinger PW, Redding RW. Effects of xylazine and ketamine hydrochloride on the electroencephalogram and the electrocardiogram in the horse. Am J Vet Res 1981; 42: 651- 659.
Sanford TD, Colby ED. Effect of xylazine and ketamine on blood pressure, heart rate and respiratory rate in rabbits. Lab Anim Sci 1980; 30: 519-523.
Rand JS, Reynolds WT, Priest J. Echocardiographic evaluation of the effects of medetomidine and xylazine in dogs. Aust Vet J 1996; 73: 41-44.
Hsu WH, Lu ZX, Hembrough FB. Effect of xylazine on heart rate and arterial blood pressure in conscious dogs, as influenced by atropine, 4-aminopyridine, doxapram, and yohimbine. J Am Vet Med Assoc 1985; 186: 153-156.
Bush M, Custer R, Smeller J, Bush LM. Physiologic measures of non-human primates during physical and chemical immobilization. J Am Vet Med Assoc 1977; 17: 866-869.
Mohammed AA, Abdelnabi MA, Modlinski JA. Evaluation of anaesthesia and reproductive performance upon diazepam and xylazine injection in rats. Anim Sci Pap Rep 2012; 30: 285-292.
Livingston A, Low J, Morris B. Effects of clonidine and xylazine on body temperature in the rat. Br J Pharmac 1984; 81: 189- 193.
Mohammed AA, Sayed MA, Abdelnabi MA. New protocol of anaesthesia using thiopental, diazepam and xylazine in white New Zealand rabbits. Aust J Basic Appl Sci 2011; 5: 1296-1300.
Yadav GU, Thorat MG, Bedarkar SN. Efficacy of xylazine as a sedative in cattle. Vet World 2008; 1: 340.
DeRossi R, Gaspar EB, Junqueira1 AL, Beretta MP. A comparison of two subarachnoid 2-agonists, xylazine and clonidine, with respect to duration of antinociception, and hemodynamic effects in goats. Small Ruminant Res 2002; 47: 103-111. Ponder SW, Clark WG. Prolonged depression of thermoregulation after xylazine administration to cats. J Vet Pharmacol Ther 1980; 3: 203-207.
Afshar FA, Baniadam A, Marashipour SP. Effect of xylazine- ketamine on arterial blood pressure, arterial blood pH, blood gases, rectal temperature, heart and respiratory rates in goats. Bull Vet Inst Play 2005; 49: 481-484.
Wixson SK, White WJ, Hughes HC, Lang CM, Marshall WK. The effects of pentobarbital, fentanyl–droperidol, ketamine– xylazine and ketamine–diazepam on core and surface body temperature regulation in adult male rats. Lab Anim Sci 1987; 37: 743-749.
Lin MT, Chen CF, Pang IH. Effect of ketamine on thermoregulation in rats. Can J Physiol Pharmacol 1978; 56: 963-967.
Joubert KE, Briggs P, Gerber D, Gottschalk RD. The analgesic and sedative effects of detomidine-butorphanol and detomidine alone in donkeys. J S Afr Vet Assoc 1990; 70: 112-118.
Hsu W. Effect of yohimbine on xylazine-induced central nervous system depression in dogs. J Am Vet Med Assoc 1983; 182: 698-699.
Vučković SM, Savić Vujović KR, Srebro DP, Medić BM, Vučetić CS, Prostranc MS, Milica Š, Prostran MS. Synergistic interaction between ketamine and magnesium in lowering body temperature in rats. Physiol Behav 2014; 127: 45-53.
Fahim I, Ismail M, Osman OH. Role of 5-hydroxytryptamine in ketamine-induced hypothermia in the rat. Br J Pharmacol 1973; 48: 570-576.
Fyffe J. Effects of xylazine on humans: a review. Aust Vet J 1994; 71: 294-295.
Xiao YF, Wang B, Wang X, Du F, Benzinou M, Wang YJ. Xylazine-induced reduction of tissue sensitivity to insulin leads to acute hyperglycemia in diabetic and normoglycemic hamadryas baboons. BMC Anesthesiol 2013; 13: 1-9.
Saha JK, Xia J, Grondin JM, Engle SK, Jakubowski JA. Acute hyperglycemia induced by ketamine/xylazine anaesthesia in rats: mechanisms and implications for preclinical models. Exp Bio Med 2005; 230: 777-784.
Brown ET, Umino Y, Loi T, Solessio E, Barlow R. Anaesthesia can cause sustained hyperglycemia in C57/BL6J mice. Vis Neurosci 2005; 22: 615-618.
Ambrisko TD, Hikasa Y. Neurohormonal and metabolic effects of medetomidine compared with xylazine in beagle dogs. Can J Vet Res 2002; 66: 42-49.
Goldfine ID, Arieff AI. Rapid inhibition of basal and glucose stimulated insulin release by xylazine. Endocrinol 1979; 105: 920-930.
Kanda T, Hikasa Y. Neurohormonal and metabolic effects of medetomidine compared with xylazine in healthy cats. Can J Vet Res 2008; 72: 278-286.
Tranquilli WJ, Thurmon JC, Neff-Davis CA, Davis LE, Benson GJ, Hoffman W, Lock TF. Hyperglycemia and hypoinsulinemia during xylazine-ketamine anaesthesia in thoroughbred horses. Am J Vet Res 1984; 45: 11-14.
Brearley JC, Dobson H, Jones RS. Investigations into the effect of two sedatives on the stress response in cattle. J Vet Pharmacol Ther 1990; 13: 367-377.
Eichner RD, Prior RL, Kvasnicka WG. Xylazine-induced hyperglycemia in beef cattle. Am J Vet Res 1979; 40: 127-129.
Symonds HW, Mallinson CB. The effect of xylazine and xylazine followed by insulin on blood glucose and insulin in the dairy cow. Vet Rec 1978; 102: 27-29.
Saha JK, Xia J, Engle SK, Chen YF, Glaesner W, Jakubowski JA. A model of controlled acute hyperglycemia in rats: effects of insulin and glucagon like peptide-1 analog. J Pharmacol Exp Ther 2006; 316: 1159-1164.
Mohseni G, Ranjbar A, Rezaei M. Oral diazepam effect on surgical patient’s postoperative blood sugar. Ann Biol Res 2012; 3: 4382-4385.