Changes in the Size Distribution of Steroidogenic and Non-steroidogenic Bovine Luteal Cells During the Oestrus Cycle
The aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3b-hydroxysteroid dehydrogenase (3b-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (
Changes in the Size Distribution of Steroidogenic and Non-steroidogenic Bovine Luteal Cells During the Oestrus Cycle
The aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3b-hydroxysteroid dehydrogenase (3b-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (