Association between polymorphisms of CYP19, CYP21, and ER1 genes and milk production traits in Black-and-White cattle

The relationships between the SNPs of the cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) gene (CYP21/HpaII), 2 polymorphic sites of estrogen receptor alpha gene (ER1/BglI and ER1/SnaBI), and milk production traits of Black-and-White cattle were analyzed. A total of 472 cows were included in the study and genotyped using PCR-RFLP. The frequencies of alleles for the Black-and-White cows were as follows: 0.923 - CYP19A, 0.077 - CYP19B, 0.042 - ER1/BglIA, and 0.958 for ER1/BglIG. For ER1/SnaBI, the frequency of allele A was 0.960 and that of allele G was 0.040. In the CYP21, all cows were genotyped as AA (no polymorphism). There were no associations between CYP19/PvuII, CYP21/HpaII, ER1/BglI, ER1/SnaBI polymorphisms, and milk production traits of the investigated cows.

Association between polymorphisms of CYP19, CYP21, and ER1 genes and milk production traits in Black-and-White cattle

The relationships between the SNPs of the cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) gene (CYP21/HpaII), 2 polymorphic sites of estrogen receptor alpha gene (ER1/BglI and ER1/SnaBI), and milk production traits of Black-and-White cattle were analyzed. A total of 472 cows were included in the study and genotyped using PCR-RFLP. The frequencies of alleles for the Black-and-White cows were as follows: 0.923 - CYP19A, 0.077 - CYP19B, 0.042 - ER1/BglIA, and 0.958 for ER1/BglIG. For ER1/SnaBI, the frequency of allele A was 0.960 and that of allele G was 0.040. In the CYP21, all cows were genotyped as AA (no polymorphism). There were no associations between CYP19/PvuII, CYP21/HpaII, ER1/BglI, ER1/SnaBI polymorphisms, and milk production traits of the investigated cows.

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  • Kobylinska, K.: Molecular forms of cytochrome P-450 in rat liver. Postepy Biochem., 1994; 40: 248-253. (article in Polish with an Abstract in English).
  • Fürbass, R., Kalbe, C., Vanselow, J.: Tissue-specifi c expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative fi rst exons. Endocrinology, 1997; 138: 2813-2819.
  • Vanselow, J., Kühn, C., Fürbass, R., Schwerin, M.: Th ree PCR/ RFLPs identifi ed in the promoter region1.1 of the bovine aromatase gene (CYP19). Anim. Genet., 1999; 30: 232-233.
  • Conley, A., Hinshelwood, M.: Mammalian aromatases. Reproduction, 2001; 121: 685-695.
  • Vanselow, J., Kühn, C., Fürbass, R., Schwerin, M.: Isolation of the bovine CYP19 promoter 1.2 and identifi cation of genetic variants. Anim. Genet., 2000; 31: 337-338.
  • Simpson, E.R., Davis, S.R.: Minireview: Aromatase and the regulation of oestrogen biosynthesis – some new perspectives. Endocrinology, 2001; 142: 4589-4594.
  • Kalbe, C., Fürbass, R., Schwerin, M., Vanselow, J.: Cis-acting elements regulating the placenta-specifi c promoter of the bovine Cyp19 gene. J. Mol. Endocrinol., 2000; 25: 265-273.
  • Fürbass, R., Said, H.M., Schwerin, M., Vanselow, J.: Chromatin structure of the bovine Cyp19 promoter 1.1. Eur. J. Biochem., 2001; 268: 1222-1227.
  • Komisarek, J., Dorynek, Z.: Polymorphism analysis of BTN, GHR, and CYP19 genes in cattle. Zesz. Nauk. Przeg. Hod., 2002; 62: 295-302. (article in Polish with an Abstract in English).
  • Barg, E., Tokarska, M., Wikiera, B., Kosowska, B.: Congenital adrenal hyperplasia – advances in diagnosis. Adv. Clin. Exp. Med., 2003; 12: 507-515.
  • Chin, K.K., Chang, S.F.: Th e -104G nucleotide of the human CYP21 gene is important for CYP21 transcription activity and protein interaction. Nucleic Acids Res., 1998; 26: 1959-1964.
  • Chung, B.C., Matteson, K.J., Miller, W.L.: Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defi nes a novel cytochrome P-450 gene family. Proc. Natl. Acad. Sci. U.S.A., 1986; 83: 4243-4247.
  • Damiani, G., Florio, S., Budelli, E., Caroli, A.: HpaII PCR-RFLP within a Bov-A2 element in the promoter of the bovine CYP21 (steroid 21-hydroxylase) gene. Anim. Genet., 2000; 31: 154- 154.
  • Damiani, G., Florio, S., Budelli, E., Bolla, P., Caroli, A.: Single nucleotide polymorphisms (SNPs) within Bov-A2 SINE in the second intron of bovine and buff alo k-casein (CSN3) gene. Anim. Genet., 2000; 31: 277-279.
  • Szreder, T., Zwierzchowski, L.: Polymorphism within the bovine estrogen receptor-α gene 5’-region. J. Appl. Genet., 2004; 45: 225-236.
  • Buske, B., Sternstein, I., Reißmann, M., Brockmann, G.: Detection of novel single-nucleotide polymorphisms (SNPs) in the CYP21 gene and association analysis of two SNPs for CYP21 and ESR2 with litter size in a commercial sow population. J. Anim. Breed. Genet., 2006; 123: 343-348.
  • Szreder, T., Żelazowska, B., Zwierzchowski, L., Pareek, C.S.: A novel nucleotide sequence polymorphism in the 5’-noncoding region of bovine estrogen receptor α gene, the RFLP-SnaBI. Biochem. Genet., 2007; 45: 255-262.
  • Kowalewska-Luczak, I.: Study of the genetic structure of dairy cattle based on polymorphism within the aromatase gene. Russ. J. Genet., 2009; 45: 811-816.
  • Jędrzejczak, M., Szatkowska, I., Zych, S., Grzesiak, W., Czerniawska-Piątkowska, E., Dybus, A.: Evaluation of associations of the polymorphism in the placenta-specifi c promoter 1.1 of the CYP19 gene in Black-and-White and Jersey cattle with milk production traits. Arch. Tierz., 2006; 49: 311-314.
  • Szreder, T., Zwierzchowski, L.: Estrogen receptors and their genes - potential markers of functional and production traits of farm animals. Mol. Biol. Rep., 2007; 34: 207-211.
  • Szreder, T., Żelazowska, B., Oprządek, J., Zwierzchowski, L.: Expression in promoter variant of the ERα gene in Bos taurus liver. Mol. Biol. Rep., 2008; 35: 65-71.
  • Okada, N., Hamada, M., Ogiwara, I., Ohshima, K.: SINEs and LINEs share common 3’ sequences: a review. Gene, 1997; 205: 229-243.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

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