The aim of this study was to assess serum asymmetric dimethyl arginine (ADMA), cardiac troponin I (cTnI), thyroxine (T4), cholesterol and triglyceride (TG) levels in dogs with normal body condition and obese dogs. A total of 40 dogs (n = 40) were included in the study and dogs were divided into two groups; 20 dogs with normal body condition and 20 obese dogs. The dogs were grouped according to the five-point body-condition scoring (BCS) system. Serum ADMA, cTnI, T4, cholesterol, and TG levels were measured in addition to routine biochemical parameters. Serum ADMA, cTnI, TG, and cholesterol levels were statistically significant between the two groups (P < 0.001, P < 0.001, P = 0.027, P = 0.006, respectively). There was a positive correlation between the serum cholesterol levels and both TG and cTnI levels among obese dogs. It is thought that the findings of this study may help future studies in determining novel biomarkers for obesity in dogs.