Antigenic Diversity of Bovine Viral Diarrhoea Viruses (BVDV) Isolated in Turkey

Sixty BVDV field strains isolated from different regions of Turkey were antigenically characterised with a panel of 16 monoclonal antibodies (mAbs) specific to viral proteins E2 and NS2-3. MAb recognition patterns were detected by an indirect peroxidase-linked antibody assay and data were subjected to computer analysis for discussing herd specificity of viral strains isolated from immunotolerant, persistently infected (IPI) cattle. For that purpose, phylogenic and statistical analyses were performed. A high level of antigenic diversity was detected among the isolates. Diversity was also exhibited in IPI strains originating from the same herd. In some herds, viruses were divided into 2 viral subpopulations that were antigenically distinct from each other. The viruses in the subpopulations were significantly different from the viruses in the twin-subpopulation, as well as from viruses in other herds. This study demonstrated epitopic properties of Turkish BVDV field strains; yet, the results of this study do not support the concept of herd specificity of the virus.

Anahtar Kelimeler:

Bovine viral diarrhoea, BVDV, monoclonal antibodies, antigenic diversity, phylogenetic analysis, herd specificity

Antigenic Diversity of Bovine Viral Diarrhoea Viruses (BVDV) Isolated in Turkey

Keywords:

Bovine viral diarrhoea, BVDV, monoclonal antibodies, antigenic diversity, phylogenetic analysis, herd specificity,

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Cay, B., Chappuis, G., Coulibaly, C., Dinter, Z., Edwards, S., Greiser-Wilke, I., Gunn, M., Have, P., Hess, G., Juntti, N., Liess, B., Mateo A., McHugh, P., Moennig, V., Nettleton, P., Wenswoort, G.: Comparative analysis of monoclonal antibodies against pestiviruses: report of an international workshop. Vet. Microbiol., 1989; 20: 123-129.
Corapi, W.V., Donis, R.O., Dubovi, E.J.: Characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. Am. J. Vet. Res. 1990; 51: 1388-1394.
Nishimori, T., Yamada, S., Shimizu, M.: Production of monoclonal antibodies against classical swine fever virus and their use for antigenic characterization of Japanese isolates. J. Vet. Med. Sci., 1996: 58: 707-710.
Vega, S., Rosell, R., Paton, D.J., Orden, J.A., de la Fuente, R.: Antigenic characterization of bovine viral diarrhoea virus isolates from Spain with a panel of monoclonal antibodies. J. Vet. Med. B, Infect. Dis. Vet. Public Health, 2000; 47: 701-706.
Peters, W., Greiser-Wilke, Moennig, V., Liess, B.: Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet. Microbiol., 1986; 12: 195- 200.
Mateo-Rosell, A.M.: A topological and functional map of epitopes on the major surface glycoprotein of the bovine viral diarrhea virus (BVDV). Inaugural-dissertation, Veterinary High School, Hannover, Germany, 1988.
Pituco, E.M.: Investigation of antigenic differences in bovine viral diarrhea virus field isolates isolated between 1959 and 1994 using monoclonal antibodies. Inaugural-dissertation, Veterinary High School, Hannover, Germany, 1995.
Bolin, S.R., Littledike, E.T., Ridpath, J.F.: Serologic detection and practical consequences of antigenic diversity among bovine viral diarrhea viruses in a vaccinated herd. Am. J. Vet. Res., 1991; 52: 1033-1037.