Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of Vaccinium arctostaphylos L.

Objectives: The scope of this study was to investigate the total phenolic, anthocyanin, and flavonoid contents and the biological properties of ethanolextract (EE), methanol extract (ME), and aqueous extract (AE) from Vaccinium arctostaphylos L.Materials and Methods: EE, ME, and AE of V. arctostaphylos were prepared. Various biological activities such as total phenolic, anthocyanin,and flavonoid contents, and antioxidant (2,2’-diphenyl-1-picrylhydrazyl ferrous ion-chelating, and ferric reducing antioxidant power assays),α-glucosidase inhibitory, anti-inflammatory, and DNA protective properties of these extracts were studied.Results: EE exhibited the highest total phenolic, anthocyanin, and flavonoid contents with 44.42±1.22 mg gallic acid equivalents/g dry weight,8.46±0.49 mg/Cyaniding-3-glucoside equivalents/g dry weight, and 9.22±0.92 mg quercetin equivalents/g dry weight, respectively. The antioxidantactivities of the extracts followed the order: EE>ME>AE. EE and ME inhibited α-glucosidase enzyme and their IC50 values were 0.301±0.002 mg/mLand 0.477±0.003 mg/mL, respectively. In addition, EE and ME were determined as noncompetitive inhibitors with inhibitory constant (Ki) values of0.48±0.02 mg/mL and 0.46±0.01 mg/mL, respectively. EE in 100 and 300 mg/kg doses caused a significant reduction in formalin-induced edema inmice, demonstrating the anti-inflammatory effect of EE. In DNA protective studies, all of the extracts protected supercoiled plasmid pBR322 DNAagainst damage caused by Fenton’s reagents due to their radical scavenging activities.Conclusion: Our results demonstrated that EE of V. arctostaphylos L. had strong antioxidant, anti-inflammatory, α-glucosidase inhibitory, andDNA protective effects, suggesting that it might be an effective medical plant to prevent or treat diseases associated with oxidative damage andinflammation.

Vaccinium arctostaphylos L.’nin Antioksidan, α-Glukozidazı İnhibe Edici, Anti-inflamatuvar ve DNA Koruyucu Özelliklerinin İncelenmesi

Amaç: Bu çalışmanın amacı Vaccinium arctostaphylos L.’den hazırlanan etanol (EE), metanol (ME) ve su (AE) ekstraktlarının toplam fenolik, antosiyanin, flavonoit içerikleri ve biyolojik özelliklerinin incelenmesidir. Gereç ve Yöntemler: V. arctostaphylos’un EE, ME ve AE ekstraktları hazırlanmıştır. Bu ekstraktların total fenolik, antosiyanin ve flavonoid içerikleri, antioksidan (2,2’-difenil-1-pikrilhidrazil, metal iyon şelatlama ve ferrik indirgeyici antioksidan gücü metotları), α-glukozidaz, anti-inflamatuvar ve DNA koruma özellikleri araştırılmıştır. Bulgular: EE, 44.42±1.22 mg galik asit eşdeğeri/g kuru ağırlık, 8.46±0.49 mg/siyanidin-3-glukozid eş değerleri/g kuru ağırlık ve 9.22±0.92 mg quercetin eş değerleri/g kuru ağırlık değerleriyle en yüksek toplam fenolik, antosiyanin ve flavonoid içeriğine sahip olduğu görülmüştür. Bununla birlikte ekstraktların antioksidan aktiviteleri sırasıyla EE>ME>AE olduğu belirlendi. EE ve ME α-glukozidaz enzimini sırasıyla 0.301±0.002 mg/mL ve 0.477±0.003 mg/mL IC50 değerleriyle inhibe etmiştir. Ayrıca, EE ve ME’nin inhibisyon sabiti (Ki ) değerleri 0.48±0.02 mg/mL ve 0.46±0.01 mg/mL bulunarak, yarışmasız inhibisyon gerçekleştirdikleri belirlenmiştir. EE’nin 100 ve 300 mg/kg dozları farelerde formalin ile indüklenen ödemi önemli derecede azalttığı belirlenmiştir. DNA koruma çalışmalarında, ekstraktlar radikal süpürme aktivitesinden dolayı Fenton reaktifiyle oluşturulan hasara karşı süpersarmal plasmid pBR322 DNA’yı korumuştur. Sonuç: Sonuçlarımız, V. arctostaphylos L.’nin EE’sinin güçlü antioksidan, anti-inflamatuvar, α-glukozidaz inhibisyon ve DNA koruyucu etkilere sahip olduğunu göstermiştir; bu, oksidatif hasar ve iltihaplanma ile ilişkili hastalıkları önlemek veya tedavi etmek için etkili bir tıbbi bitki olabileceğini düşündürmektedir.

___

1. Seebaluck-Sandoram R, Lall N, Fibrich B, Bloom van Staden A, Mahomoodally F. Antibiotic-potention, antioxidant, cytotoxic, antiinflammatory and anti-acetylcholinesterase potential of Antidesma madagascariense Lam. (Euphorbiaceae). S Afr J Bot. 2017;111:194-201.

2. Supasuteekul C, Nonthitipong W, Tadtong S, Likhitwitayawuid K, Tengamnuay P, Sritularak B. Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis. Rev Bras Farmacog. 2016;26:312-320.

3. Hyun TK, Kim HC, Ko YJ, Kim JS. Antioxidant, α-glucosidase inhibitory and anti-inflammatory effects of aerial parts extract from Korean crowberry (Empetrum nigrum var japonicum). Saudi J Biol Sci. 2016;23:181-188.

4. Feng CY, Wang WW, Ye JF, Li SS, Wu Q, Yin DD, Li B, Xu YJ, Wang LS. Polyphenol profile and antioxidant activity of the fruit and leaf of Vaccinium glaucoalbum from the Tibetan Himalayas. Food Chem. 2017;219:490-495.

5. Ahmadi A, Khalili M, Mashaee F, Nahri-Niknafs B. The effects of solvent polarity on hypoglycemic and hypolipidemic activities of Vaccinium arctostaphylos L. Unripe fruits. Pharm Chem J. 2017;50:746-752.

6. Kraujalyte V, Venskutonis PR, Pukalskas A, Cesoniene L, Daubaras R. Antioxidant properties, phenolic composition and potentiometric sensor array evaluation of commercial and new blueberry (Vaccinium corymbosum) and bog blueberry (Vaccinium uliginosum) genotypes. Food Chem. 2015;188:583-590.

7. Cambers BK, Camire ME. Can cranberry supplementation benefit adults with type 2 diabetes. Diabetes Care. 2003;26:2695-2696.

8. Kraft TFB, Schmidt BM, Yousef GG, Knight CTG, Cuendet M, Kang YH, Pezzuto JM, Siegler DS, Lila MA. Chemopreventive potential of wild lowbush blueberry fruits in multiple stages of carcinogenesis. J Food Sci. 2005;70:159-166.

9. Krikorian R, Shidler MD, Nash TA, Kalt W, Vinqvist-Tymchuk MR, Shukitt Hale B, Joseph JA. Blueberry supplementation improves memory in older adults. J Agric Food Chem. 2010;58:3996-4000.

10. Liu Y, Song X, Han Y, Zhou F, Zhang D, Ji B, Hu J, Lv Y, Cai S, Wei Y, Gao F, Jia X. Identification of anthocyanin components of wild Chinese blueberries and amelioration of light induced retinal damage in pigmented rabbit using whole berries. J Agric Food Chem. 2011;59:356- 363.

11. Mohaddese M, Kazempour N, Taghizadeh M. In vitro antimicrobial and antioxidant activity of Vaccinium arctostaphylos L. extracts. Journal of Biologically Active Products from Nature. 2013;3:241-247.

12. Güder A, Engin MS, Yolcu M, Gür M. Effect of processing temperature on the chemical composition and antioxidant activity of Vaccinium arctostaphylos fruit and their jam. J Food Process Preserv. 2014;38:1696- 1704.

13. Jooyandeh H, Noshad M, Khamirian RA. Modeling of ultrasoundassisted extraction, characterization and in vitro pharmacological potential of polysaccharides from Vaccinium arctostaphylos L. Int J Biol Macromol. 2018;107:938-948.

14. Ayaz FA, Hayırlıoğlu Ayaz S, Gruz J, Novak O, Strnad M. Separation, characterization, and quantitation of phenolic acids in a little-known blueberry (Vaccinium arctostaphylos L.) fruit by HPLC-MS. J Agric Food Chem. 2005;53:8116-8122.

15. Latti AK, Kainulainen PS, Hayırlıoğlu-Ayaz S, Ayaz FA, Riihinen KR. Characterization of anthocyanins in caucasian blueberries (Vaccinium arctostaphylos L.) native to Turkey. J Agric Food Chem. 2009;57:5244- 5249.

16. Deliorman Orhan D, Orhan N. Assessment of In Vitro Antidiabetic and Antioxidant Effects of Helianthus tuberosus, Cydonia oblonga and Allium porrum. Turk J Pharm Sci. 2016;13:181-188.

17. Şöhretoğlu D, Sari S, Soral M, Barut B, Özel A, Liptaj T. Potential of Potentilla inclinata and its polyphenolic compounds in α-glucosidase inhibition: Kinetics and interaction mechanism merged with docking simulations. Int J Biol Macromol. 2018;108:81-87.

18. International Diabetes Federation, Diabetes Atlas. www.idf.org/ diabetesatlas (Accessed 9 March 2018) 2017.

19. Sulistiyani, Safithri M, Sari YP. Inhibition of α-glucosidase activity by ethanolic extract of Melia azedarach L. leaves. IOP Conf Ser Earth Environ Sci. 2016;31:1-5.

20. Zhang J, Zhao S, Yin P, Yan L, Han J, Shi L, Zhou X, Liu Y, Ma C. α-Glucosidase Inhibitory Activity of Polyphenols from the Burs of Castanea mollissima Blume. Molecules. 2014;19:8373-8386.

21. Barut EN, Barut B, Engin S, Yıldırım S, Yaşar A, Türkiş S, Özel A, Sezen FS. Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Turk J Biochem. 2017;42:493-502.

22. Keser S, Çelik S, Türkoğlu S, Yılmaz O, Turkoğlu I. Antioxidant activity, total phenolic and flavonoid content of water and ethanol extracts from Achillea millefolium L. Turk J Pharm Sci. 2013;10:385-392.

23. Cheng GW, Breen PJ. Activity of phenylalanine ammonialyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J Am Soc Hortic Sci. 1991;116:865-869.

24. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10:178-182.

25. Bakar F, Bahadır Acıkara Ö, Ergene B, Nebioğlu S, Saltan Çitoğlu G. Antioxidant activity and phytochemical screening of some Asteraceae Plants. Turk J Pharm Sci. 2015;12:123-132.

26. Chua MT, Tung YT, Chang ST. Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophleum. Bioresour Technol. 2008;99:1918-1925.

27. Oyaizu M. Studies on products of browning reactions-antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986;44:307-315.

28. da Silva Pinto M, Kwon YI, Apostolidis E, Lajolo FM, Genovese MI, Shetty K. Functionality of bioactive compounds in brazilian strawberry (Fragaria × An(anassa Duch.) cultivars: evaluation of hyperglycemia and hypertension potential using in vitro models. J Agric Food Chem. 2008;56:4386-4392.

29. Lineweaver H, Burk D. The determination of enzyme dissociation constant. J Am Chem Soc. 1934;56:658-666.

30. Şöhretoglu D, Sari S, Özel A, Barut B. α-Glucosidase inhibitory effect of Potentilla astracanica and some isoflavones: inhibition kinetics and mechanistic insights through in vitro and in silico studies. Int J Biol Macromol. 2017;105:1062-1070.

31. Yeung SY, Lan WH, Huang CS, Lin CP, Chan CP, Chang MC, Jeng JH. Scavenging property of three cresol isomers against H2O2, hypochlorite, superoxide and hydroxyl radicals. Food Chem Toxicol. 2002;40:1403-1413.

32. Barut B, Demirbaş Ü, Özel A, Kantekin H. Novel water soluble morpholine substituted Zn(II) phthalocyanine: Synthesis, characterization, DNA/ BSA binding, DNA photocleavageand topoisomerase I inhibition. Int J Biol Macromol. 2017;105:499-508.

33. Kumar T, Jain V. Antinociceptive and anti-inflammatory activities of Bridelia retusa methanolic fruit extract in experimental animals. Scientific World Journal. 2014;2014:890151.

34. Kumar S, Sandhir R, Ojha S. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Res Notes. 2014;7:560.

35. Alam MA, Zaidul IS, Ghafoor K, Sahena F, Hakim MA, Rafii MY, Abir HM, Bostanudin MF, Perumal V, Khatib A. In vitro antioxidant and α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans. BMC Complement Altern Med. 2017;17:181.

36. Saral Ö, Ölmez Z, Şahin H. Comparison of Antioxidant Properties of Wild Blueberries (Vaccinium arctostaphylos L. and Vaccinium myrtillus L.) with Cultivated Blueberry Varieties (Vaccinium corymbosum L.) in Artvin Region of Turkey. Turk J Ag Food Sci Techn. 2015;3:40-44.

37. Hasanloo T, Sepehrifar R, Hajimehdipoor H. Levels of phenolic compounds and their effects on antioxidant capacity of wild Vaccinium arctostaphylos L. (Qare-Qat) collected from different regions of Iran. Turk J Biol. 2011;35:371-377.

38. Yıldırım S, Kadıoğlu A, Sağlam A, Yaşar A, Sellitepe HE. Fast determination of anthocyanins and free pelargonidin in fruits, fruit juices, and fruit wines by high-performance liquid chromatography using a core-shell coloumn. J Sep Sci. 2016;39:3927-3935.

39. Raffa D, Maggio B, Raimondi MV, Plescia F, Daidone G. Recent discoveries of anticancer flavonoids. Eur J Med Chem. 2017;142:213- 228.

40. Kazeem MI, Ashafa AOT. In vitro antioxidant and antidiabetic potentials of Dianthus basuticus Burtt Davy whole plant extracts. J Herb Med. 2015;5:158-164.

41. Jimenez-Suarez V, Nieto-Camacho A, Jimenez-Estrada M, Alvarado Sanchez B. Anti-inflammatory, free radical scavenging and alphaglucosidase inhibitory activities of Hamelia patens and its chemical constituents. Pharm Biol. 2016;54:1822-1830.

42. Zlotek U, Szychowski KA, Swieca M. Potential in vitro antioxidant, antiinflammatory, antidiabetic, and anticancer effect of arachidonic acidelicited basil leaves. J Funct Foods. 2017;36:290-299.

43. Feshani AM, Kouhsari SM, Mohammadi S. Vaccinium arctostaphylos, a common herbal medicine in Iran: Molecular and biochemical study of its antidiabetic effects on alloxan-diabetic Wistar rats. J Ethnopharmacol. 2011;133:67-74.

44. Mohammad FE, Hasan WA, Mohamed EG. Natural antioxidant flavonoids in formalin-induced mice paw inflammation; inhibition of mitochondrial sorbitol dehydrogenase activity. J Biochem Mol Toxicol. 2017;31:21896.

45. Bowen-Forbes CS, Zhang Y, Nair MG. Anthocyanin content, antioxidant, anti-inflammatory and anticancer propertiesof blackberry and raspberry fruits. J Food Compos Anal. 2010;23:554-560.

46. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed. 2013;3:623-627.

47. Jiang Y, Han W, Shen T, Wang MH. Antioxidant activity and protection from DNA damage by water extract from pine (Pinus densiflora) bark. Prev Nutr Food Sci. 2012;17:116-121.

48. Kada S, Bouriche H, Senator A, Demirtaş I, Özen T, Çeken Toptanci B, Kızıl G, Kızıl M. Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation. Pharm Biol. 2017;55:330-337.

___

Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

6.2b278

Sayıdaki Diğer Makaleler

Phytotherapy as a Complementary Medicine for Multiple Sclerosis

Zahra RABIEI

Study of the Tableting Properties of MCR, a Newly Coprocessed Cellulose-based Direct Compression Excipient

Salah ALY

Optimization of Thiazolidone Scaffolds Using Pocket Modeling for Development of Potential Secretory System Inhibitors of Mycobacterium tuberculosis

Shivratna V. KHARE, Sujata P. CHOUDHARI, Siddharth P. PHALLE, Santosh S. KUMBHAR, Prafulla B. CHOUDHARI, Sambhaji R. MASAL, Aakash K. PATIL, Rakesh P. DHAVALE, Durgacharan A. BHAGWAT, Atul M. KADAM

Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of Artemisia campestris from Algeria

Boulanouar BAKCHICHE, Abdelaziz GHERIB, Maria Ros rio BRONZE, Mosad A. GHAREEB

Screening of Sacrificial Excipients for Arresting Devitrification of Itraconazole from Solid Dispersion

Bhargavi M. PATEL, Mukesh C. GOHEL, Vaishali T. THAKKAR, Lalji H. BALDANIYA, Ruby R. CHRISTIAN, Tejal R. GANDHI

Electroanalytical Determination of the Antiinflammatory Drug Tenoxicam in Pharmaceutical Dosage Forms

Fatma AĞIN, Sena ATAL

Flavonoid Glycosides from Heracleum pastinaca Fenzl

Perihan GÜRBÜZ

Antimicrobial and Anti-Inflammatory Activity of Some Lathyrus L. (Fabaceae) Species Growing in Turkey

Hajar HEYDARİ, Gülçin SALTAN İŞCAN, Müjde ERYILMAZ, Özlem BAHADIR ACIKARA, Sezen YILMAZ SARIALTIN, Mehmet TEKİN, Tülay ÇOBAN

Essential Oil and Fatty Acid Composition of Endemic Gypsophila laricina Schreb. from Turkey

Hüseyin SERVİ, Betül EREN KESKİN, Sezgin ÇELİK, Ümit BUDAK, Büşra KABABIYIK

Investigation of the Polyphenol Composition, Biological Activities, and Detoxification Properties of Some Medicinal Mushrooms from Turkey

Naznoosh SHOMALI, Okan ONAR, Tuğçe ALKAN, Nergiz DEMİRTAŞ, Ilgaz AKATA, Özlem YILDIRIM