In Vivo Antioxidant Activity of Different Fractions of Indigofera Barberi Against Paracetamol-induced Toxicity in Rats

Objectives: To evaluate the in vivo antioxidant activity of chloroform extract fractions of Indigofera barberi (whole plant) against paracetamolinduced toxicity in rats.Materials and Methods: For 7 days, rats were treated with different chloroform extract fractions and toxicity was induced with a single dose ofparacetamol by intraperitoneal injection. The group of animals pretreated with 100 mg/kg p.o of fraction D of Indigofera barberi improved significantlyin terms of hepatic superoxide dismutase (SOD), catalase and peroxidase activities, and glutathione levels compared to the control group.Results: The hepatic SOD, catalase, peroxidase activities, and glutathione levels in the animal groups treated with paracetamol were 33.6±0.09 µ/mgprotein, 5.5±0.23 µ/mg protein, 0.131±0.15 µ/mL, and 46.1±5.81 µM, respectively. Hepatic SOD, catalase, peroxidase, and glutathione in the fraction Dtreated group were 61.8±0.07 µ/mg protein, 10.6±0.16 µ/mg protein, 0.913±0.23 µ/mL, and 87.6±1.4 micro molar, respectively. Therefore, the presentstudy revealed that fraction D of Indigofera barberi has significant in vivo antioxidant activity and can be used to protect tissue from oxidative stress.Conclusion: From the results, fraction D of Indigofera barberi at a dose of 100 mg/kg, p.o., improved the SOD, catalase and peroxidase activities, andglutathione levels significantly. Based on this study, we can conclude that fraction D of Indigofera barberi possesses in vivo antioxidant activity andcan be employed in protecting tissue from oxidative stress.

Sıçanlarda Parasetamol ile İndüklenen Toksisiteye Karşı Indigofera Barberi’nin Farklı Fraksiyonlarının İn Vivo Antioksidan Aktivitesi

Amaç: Sıçanlarda parasetamol ile indüklenen toksisiteye karşı Indigofera barberi’nin (tüm bitki) kloroform ekstre fraksiyonlarının in vivo antioksidan aktivitesinin belirlenmesi. Gereç ve Yöntemler: Yedi gün boyunca sıçanlara farklı kloroform ekstrakları uygulanmıştır ve toksisite intraperitoneal tek doz parasetamol uygulaması ile indüklenmiştir. 100 mg/kg p.o. fraksiyon D ile ön uygulaması alan hayvanlar hepatik süperoksit dismutaz (SOD), katalaz ve peroksidaz aktiviteleri ve glutatyon düzeyleri açısından kontrol grubuna göre belirgin bir şekilde iyileşmişlerdir. Bulgular: Parasetamol uygulanan grupta hepatik SOD, katalaz, ve peroksidaz aktiviteleri ve glutatyon düzeyleri sırasıyla 33,6±0,09 µ/mg protein, 5,5±0,23 µ/mg protein, 0,131±0,15 µ/mg protein ve 46,1±5,81 µM olarak bulunmuştur. Fraksiyon D uygulanan grupta hepatik SOD, katalaz ve peroksidaz aktiviteleri ve glutatyon düzeyleri sırasıyla 61,8±0,07 U/mg protein, 10,6±0,16 µ/mg protein, 0,913±0,23 µ/mg protein ve 87,6±1,4 µM bulunmuştur. Bu nedenle, bu çalışma Indigofera barberi’de elde edilen fraksiyon D’nin belirgin bir in vivo antioksidan aktivitesi olduğunu ortaya koymuştur ve dokuyu oksidatif stresten korumak için kullanılabilir. Sonuç: Bu sonuçlar, 100 mg/kg, p.o. dozda Indigofera barberi’den elde edilen fraksiyon D, SOD, atalaz ve peroksidaz aktiviteleri ve glutatyon düzeylerini belirgin bir şekilde düzeltmiştir. Bu çalışmaya dayanarak, Indigofera barberi’den elde edilen fraksiyon D’nin in vivo antioksidan aktivitesinin olduğu sonucuna varabiliriz ve dokuyu oksidatif stresten korumak için kullanılabileceği söylenebilir.

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Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

6.6b294

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