Evaluation of Nootropic Activity of Limonia acidissima Against Scopolamine-induced Amnesia in Rats

Objectives: The present study aimed to evaluate the nootropic activity of Limonia acidissima in rats. Materials and Methods: Methanolic extract of Limonia acidissima was used to evaluate nootropic activity, piracetam (200 mg/kg, i.p.) was used as a standard, and scopolamine (1 mg/kg, i.p.) was used to induce amnesia. The effect of drugs on learning and memory in rats was evaluated by using the Y-maze task and elevated plus maze on scopolamine-induced amnesia models. Locomotor activity was performed using an actophotometer. Also, levels of acetylcholinestrease, including histopathological examination of rat brains, were assessed. Results: Methanolic extract of Limonia acidissima showed increased alteration of the behavior response and percentage spontaneous alteration with the Y-maze task. In the elevated plus maze scopolamine-induced amnesia model, methanolic extract of Limonia acidissima showed a decrease in transfer latency, which is indicative of cognition improvement. Methanolic extract increased locomotor activity in rats and decreased the levels of acetylcholinestrease enzyme significantly. A histopathological study with both low and high doses of extract showed effective regenerative scores as compared to normal control, negative control and standard treatment. Conclusion: The results suggested that the administration of methanolic extract of Limonia acidissima enhances learning and memory in different experimental models. The histopathological study revealed the neuroprotective property of the extract. The study indicates that the extract may be used in the treatment of Alzheimer’s disease. Key words: Nootropic activity, Limonia acidissima, Alzheimer’s disease, piracetam, scopolamine

Limonia acidissima’nın Sıçanlarda Skopolamin ile İndüklenen Amneziye Karşı Nootropik Aktivitesinin Değerlendirilmesi

Amaç: Bu çalışmada, sıçanlarda Limonia acidissima’nın nootropik aktivitesinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Nootropik aktiviteyi değerlendirmek için Limonia acidissima’nın metanol ekstresi, standart olarak pirasetam (200 mg/kg, i.p.) ve amneziyi indüklemek için skopolamin (1 mg/kg, i.p.) kullanıldı. İlaçların sıçanlarda öğrenme ve hafıza üzerindeki etkisi, skopolamin ile indüklenen amnezi modelinde Y-labirent testi ve yükseltilmiş artı labirent testi kullanılarak değerlendirildi. Lokomotor aktivite, bir aktofotometre kullanılarak gerçekleştirildi. Ayrıca, sıçan beyinlerinin asetilkolinestreaz aktivitesinin değerlendirilmesi de dahil olmak üzere histopatolojik incelemesi yapıldı. Bulgular: Y-labirent testi ile Limonia acidissima’nın metanol ekstresinin davranış tepkisinde ve yüzde spontan değişikliklerde artışa neden olduğu gösterildi. Skopolamin ile indüklenen amnezi modelinin kullanıldığı yükseltilmiş artı labirent testinde, Limonia acidissima’nın metanol ekstresinin, biliş gelişiminin göstergesi olan transfer gecikmesinde bir azalmaya yol açtığı gösterildi. Metanol ekstresi, sıçanlarda lokomotor aktiviteyi artırdı ve asetilkolinestreaz enzim aktivitesini önemli ölçüde düşürdü. Hem düşük hem de yüksek dozda ekstreyle yapılan histopatolojik çalışmada, normal kontrol, negatif kontrol ve standart tedaviye kıyasla efektif rejeneratif skorlar elde edildi. Sonuç: Sonuçlar, Limonia acidissima’nın metanol ekstresinin uygulanmasının farklı deneysel modellerde öğrenmeyi ve hafızayı geliştirdiğini göstermiştir. Histopatolojik çalışma, ekstrenin nöroprotektif özellikte olduğunu ve ekstrenin Alzheimer hastalığının tedavisinde kullanılabileceğini göstermiştir. Anahtar kelimeler: Nootropik aktivite, Limonia acidissima, Alzheimer hastalığı, pirasetam, skopolamin

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Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

6.6b293

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Evaluation of Nootropic Activity of Limonia acidissima Against Scopolamine-induced Amnesia in Rats

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