Bazı Salvia Türlerinde Rozmarinik Asit’in Kantitatif Analizi İçin Valide Edilmiş YPİTK Yöntemi

Kafeik asit ve 3,4-dihidroksifenillaktik asit esteri olan rozmarinik asit (RA) özellikle Lamiaceae familyasının alt familyası olan Nepetoideae' ye bağlı Salvia türlerinin en önemli fenolik bileşiklerindendir. Bu çalışmamızda son zamanlarda pek çok aktiviteden sorumlu olduğu öne sürülen RA' nın bitki ekstrelerinde miktar tayininin kolayca yapılabilmesi için hızlı, basit ve hassas bir metod geliştirdik. Geliştirdiğimiz metodu Türkiye' nin çeşitli bölgelerinden topladığımız ve satın aldığımız yedi farklı Salvia türüne (S. candidissima, S. dichroantha, S. heldreichiana, S. sclarea, S. tomentosa, S. triloba ve S. officinalis) uyguladık. Bitkilerin toprak üstü kısımlarının metanol ekstreleri silika jel 60 F254 YPİTK plaklarına mobil faz olarak toluen: etil asetat: formik asit (5:4:1) kullanılarak uygulanmış ve 330 nm' de RA miktarı densitometrik olarak tayin edilmiştir. Sonuç olarak geliştirdiğimiz ve valide ettiğimiz yöntemimizin, RA miktar tayini için basit, güvenilir ve de kullanışlı bir yöntem olduğu kanıtlanmıştır.

Validated HPTLC Method for the Quantitative Analysis of Rosmarinic Acid in Several Salvia Sp

Rosmarinic acid (RA), which is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid, is one of the most significant phenolic compound in the family Lamiaceae, which is restricted to the subfamily Nepetoideae, including Salvia species. Since various biological effects have been attributed to RA content of medicinal plants, has recently gained a great importance. We aimed to develop a rapid, simple and sensitive method for the quantitative determination of RA in extracts. The method was then practiced for comparative analysis of RA contents in seven Salvia species; S. candidissima, S. dichroantha, S. heldreichiana, S. sclarea, S. tomentosa, S. triloba and as well as the official sage S. officinalis. The methanol extracts obtained from the aerial parts of the plant materials were submitted to chromatographic separation on silica gel 60 F254 HPTLC plates with toluene: ethyl acetate:formic acid (5:4:1) as mobile phase and the densitometric detection of RA was carried out at 330 nm by HPTLC. Method was then validated in terms of accuracy, precision, repeatability, reproducibility, linearity, limit of detection/quantification, sensitivity and specificity. The newly developed HPTLC method provides a powerful approach to estimate RA content which is a generally phytomarker in many plant extracts

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Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

6.6b293

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