Antioxidant, Anti-inflammatory, and Analgesic Activities of Alcoholic Extracts of Ephedra nebrodensis From Eastern Algeria

Objectives: Ephedra nebrodensis (Ephedraceae) presents a wide range of biological activities. It is used to treat respiratory problems and hepatic pathologies in traditional medicine. The aim of this study is to evaluate the antioxidant, in vitro and in vivo anti-inflammatory and analgesic properties of two hydro-alcoholic extracts of E. nebrodensis in mice. Materials and Methods: The antioxidant capacity of hydro-methanolic (HM) and hydro-ethanolic (HE) extracts of E. nebrodensis was evaluated via assays of their superoxide radical scavenging capacity and ferrous ion chelating activity. The in vitro anti-inflammatory activity of the extracts (5, 10, and 20 mg/kg) was also determined using the bovine serum albumin denaturation test. Croton oil-induced ear edema was then employed to evaluate the in vivo anti-inflammatory effect of the extracts (200 and 400 mg/kg). Finally, the analgesic activity of the extracts (200 and 400 mg/ kg) was determined by the acetic acid-induced torsion test. Results: The hydro-alcoholic extracts of E. nebrodensis present significant antioxidant activity. The HE and HM could inhibit protein denaturation by 82.99%±20.21% and 56.25%±2.12%, respectively. The extracts (HM and HE) also show strong anti-inflammatory effects in vivo and could reduce ear edema by 70.37%±2.00% and 72.22%±1.94%, respectively. The HM extract (72.51%±2.43%) demonstrates greater pain inhibitory effects than HE (70.76%±2.58%). Conclusion: The hydro-alcoholic extracts of E. nebrodensis produce antioxidant, anti-inflammatory, and analgesic effects. These results confirm the traditional use of the herb in the treatment of various diseases.

Doğu Cezayir’den Ephedra nebrodensis Bitkisinin Alkol ile Hazırlanan Ekstrelerinin Antioksidan, Anti-inflamatuvar ve Analjezik Aktiviteleri

Amaç: Ephedra nebrodensis (Ephedraceae) bitkisinin çok çeşitli biyolojik aktivitesi bulunmaktadır. Geleneksel tıpta solunum problemlerini ve karaciğer patolojilerini tedavi etmek için kullanılır. Bu çalışmanın amacı, in vitro ve in vivo (farelerde) koşullarda E. nebrodensis’in iki hidro-alkollü ekstresinin antioksidan, anti-inflamatuvar ve analjezik özelliklerini değerlendirmektir. Gereç ve Yöntemler: E. nebrodensis’in hidro-metanolik (HM) ve hidro-etanolik (HE) ekstrelerinin antioksidan kapasitesi, süperoksit radikal süpürme kapasiteleri ve demirli iyon şelatlama aktivitelerinin belirlenmesi ile değerlendirildi. Ekstrelerin (5, 10 ve 20 mg/kg) in vitro anti-inflamatuvar aktivitesi de sığır serum albümin denatürasyon testi kullanılarak belirlendi. Daha sonra ekstrelerin (200 ve 400 mg/kg) in vivo anti-inflamatuvar etkisini değerlendirmek için kroton yağı ile indüklenen kulak ödemi modeli kullanıldı. Son olarak ekstrelerin analjezik aktivitesi (200 ve 400 mg/kg), asetik asit ile indüklenen kıvranma testi ile belirlendi. Bulgular: E. nebrodensis’in hidro-alkolik ekstreleri, önemli antioksidan aktiviteye sahiptir. HE ve HM, protein denatürasyonunu sırasıyla %82,99±%20, 21 ve %56,25±%2,12 oranında inhibe edebilir. Ekstreler (HM ve HE) ayrıca in vivo olarak güçlü anti-inflamatuvar etkiler gösterir ve kulak ödemini sırasıyla %70,37±%2,00 ve %72,22±%1,94 oranında azaltabilir. HM ekstresi (%72,51±%2,43) HE’den (%70,76±%2,58) daha yüksek analjezik aktiviteye sahiptir. Sonuç: E. nebrodensis’in hidro-alkolik ekstreleri, antioksidan, anti-inflamatuvar ve analjezik etkiler üretir. Bu sonuçlar, bitkinin çeşitli hastalıkların tedavisinde geleneksel kullanımını doğrulamaktadır.

Kaynakça

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Kaynak Göster

Turkish Journal of Pharmaceutical Sciences
  • ISSN: 1304-530X
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 2000

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