Melek BÜYÜKEREN, Hasan Tolga ÇELİK, Oytun PORTAKAL, Erdem FADILOĞLU, Gökçen ÖRGÜL, Şule YİĞİT, M. Sinan BEKSAÇ, Murat YURDAKÖK

Cord blood delta neutrophil index values of term neonates

In this study, we aimed to demonstrate cord blood immature granulocyte (IG) count and delta neutrophil index (DNI) values for term neonates. This retrospective study consisted of 126 term newborns born between July 2017 and December 2017. Cord blood samples were collected during delivery and IG count together with DNI values were obtained. “Beckman Coulter DXH800 System Hematology Analyzer” was used for analysis and calculations. The median DNI value was found to be 1.0 (interquantile range(IQR) 0.5-1.8%) and the median gestational age at delivery was 38.4 (IQR 37.6-39.0) weeks. The median birth weight and IG count were 3250 (IQR 2955–3593) g and 66 (IQR 26.5-112.3)/mm3, respectively. In conclusion, we believe that determining the normal laboratory reference values of IG count or DNI, which are important potential diagnostic markers for neonatal sepsis, will contribute to future studies on the diagnosis of neonatal sepsis.

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1.Oza S, Lawn JE, Hogan DR, Mathers C, Cousens SN. Neonatal cause-of-death estimates for the early and late neonatal periods for 194 countries: 2000-2013. Bull World Health Organ 2015; 93: 19-28.

2.Mukhopadhyay S, Puopolo KM. Risk assessment in neonatal early-onset sepsis. Semin Perinatol 2012; 36: 408-415.

3.Connell TG, Rele M, Cowley D, Buttery JP, Curtis N. How reliable is a negative blood culture result? Volume of blood submitted for culture in routine practice in a children's hospital. Pediatrics 2007; 119: 891-896.

4.Chauhan N, Tiwari S, Jain U. Potential biomarkers for effective screening of neonatal sepsis infections: an overview. Microb Pathog 2017; 107: 234-242.

5.Hedegaard SS, Wisborg K, Hvas AM. Diagnostic utility of biomarkers for neonatal sepsis--a systematic review. Infec Dis (Lond) 2015; 47: 117-124.

6.Tang BM, Eslick GD, Craig JC, McLean AS. Accuracy of procalcitonin for sepsis diagnosis in critically ill patients: systematic review and meta-analysis. Lancet Infect Dis 2007; 7: 210-217.

7.Lee SM, Eun HS, Namgung R, Park MS, Park KI, Lee C. Usefulness of the delta neutrophil index for assessing neonatal sepsis. Acta Paediatr (Oslo) 2013; 102: e13-e16.

8.Wiland EL, Sandhaus LM, Georgievskaya Z, Hoyen CM, O'Riordan MA , Nock ML. Adult and child automated immature granulocyte norms are inappropriate for evaluating early-onset sepsis in newborns. Acta Paediatr (Oslo, Norway: 1992) 2014; 103: 494-497.

9.Seok Y, Choi JR, Kim J, et al. Delta neutrophil index: a promising diagnostic and prognostic marker for sepsis. Shock (Augusta) 2012; 37: 242-246.

10.Park BH, Kang YA, Park MS, et al. Delta neutrophil index as an early marker of disease severity in critically ill patients with sepsis. BMC Infect Dis 2011; 11: 299.

11.Nierhaus A, Klatte S, Linssen J, et al. Revisiting the white blood cell count: immature granulocytes count as a diagnostic marker to discriminate between SIRS and sepsis--a prospective, observational study. BMC Immunol 2013; 14: 8.

12.Cimenti C, Erwa W, Müller W, Resch B. The role of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis. Neonatal Bacterial Infection: IntechOpen, 2013. http://dx.doi.org/10.5772/54531.

13.Wynn JL, Wong HR, Shanley TP, Bizzarro MJ, Saiman L, Polin RA. Time for a neonatal–specific consensus definition for sepsis. Pediatr Crit Care Med 2014; 15: 523-528.

14.Mahale R, Dutta S, Ahluwalia J, Kishore SS, Narang A. Baseline illness severity does not alter accuracy of neonatal sepsis screen. Am J Perinatol 2010; 27: 327-332.

15.Nahm CH, Choi JW, Lee J. Delta neutrophil index in automated immature granulocyte counts for assessing disease severity of patients with sepsis. Ann Clin Lab Sci 2008; 38: 241-246.

16.Wang J, Yu J, Fan J, He Y, Dong W, Wang Z. Evaluation of altitude-appropriate reference ranges for neutrophils in diagnosis of sepsis in very low birth weight infants: a multicenter retrospective study. PLoS One 2017; 12: e0171571.