Roles of glycoprotein glycosylation in the pathogenesis of an endemic osteoarthritis, Kashin-Beck disease, and effectiveness evaluation of sodium hyaluronate treatment

Background/aim: We aimed to explore the roles of glycoprotein glycosylation in the pathogenesis of Kashin-Beck disease KBD , and evaluated the effectiveness of sodium hyaluronate treatment. Materials and methods: Blood and saliva were collected from KBD patients before and after the injection of sodium hyaluronate. Normal healthy subjects were included as controls. Saliva and serum lectin microarrays and saliva and serum microarray verifications were used to screen and confirm the differences in lectin levels among the three groups. Results: In saliva lectin microarray, bindings to Sophora japonica agglutinin SJA , Griffonia Bandeiraea simplicifolia lectin I GSL-I , Euonymus europaeus lectin EEL , Maackia amurensis lectin II MAL-II , Sambucus nigra lectin SNA , Hippeastrum hybrid lectin HHL , and Aleuria aurantia lectin AAL were higher in the untreated KBD patients than in the control group. Increased levels of HHL, MAL-II, and GSL-I in the untreated KBD patients discriminated them in particular from the treated ones. Jacalin was lower in the untreated KBD patients compared to the treated KBD and control groups. In serum lectin microarray, HHL and peanut agglutinin PNA were increased in the untreated KBD group in comparison to the control one. AAL, Phaseolus vulgaris agglutinin E+L PHA- E+L , and Psophocarpus tetragonolobus lectin I PTL-I were lower in the untreated KBD patients compared to the treated KBD and control groups. Hyaluronate treatment appeared to normalize SNA, AAL, and MAL-II levels in saliva, and HHL, PNA, AAL, PTL-I, and PHA-E+L levels in serum. Saliva reversed microarray verification confirmed significant differences between the groups in SNA and Jacalin, in particular for GSL-I levels, while serum reversed microarray verification indicated that HHL, PNA, and AAL levels returned to normal levels after the hyaluronate treatment. Lectin blot confirmed significant differences in HHL, AAL, and Jacalin in saliva, and HHL, PNA, PHA-E+L, and AAL in serum. Conclusion: HHL in saliva and serum may be a valuable diagnostic biomarker of KBD, and it may be used as follow-up for the hyaluronate treatment.

___

  • 1. Wang S, Guo X, Wang W, Wang S. Genome-wide study identifies the regulatory gene networks and signaling pathways from chondrocyte and peripheral blood monocyte of Kashin– Beck disease. Genes to Cells 2012; 17(8): 619-632. doi: 10.1111/j.1365-2443.2012.01620.x
  • 2. Gao Z, Guo X, Chen J, Duan C, Ma W et al. The effect of hyaluronic acid on the expression of IL-1β,TNF-α in chondrocytes of Kashin–Beck disease. Shaanxi Medical Journal 2015; 44(7): 774-778. doi: 10.3969/j.issn.1000- 7377.2015.07.002
  • 3. Gao Z, Guo X, Chen J, Duan C, Ma W et al. Effect of hyaluronic acid on collagen II and aggrecan mRNA expression in Kashin– Beck disease chondrocytes cultured in vitro. Journal of Clinical Rehabilitative Tissue Engineering Research 2009; 13(46): 9061- 9065. doi:10.3969/j.issn.1673-8225.2009.46.012
  • 4. Xia CT, Yu FF, Ren FL, Fang H, Guo X. Hyaluronic acid and glucosamine sulfate for adult Kashin–Beck disease: a clusterrandomized, placebo-controlled study. Clinical Rheumatology 2016; 35(5): 1263-1270. doi: 10.1007/s10067-014-2809-6
  • 5. Takeuchi H, Haltiwanger RS. Role of glycosylation of Notch in development. Seminars in Cell and Developmental Biology 2010; 21(6): 638-645. doi: 10.1016/j.semcdb.2010.03.003
  • 6. Manning JC, Romero A, Habermann FA, Garcia Caballero G, Kaltner H et al. Lectins: a primer for histochemists and cell biologists. Histochemistry and Cell Biology 2017; 147(2): 199- 222. doi: 10.1007/s00418-016-1524-6
  • 7. Corfield AP, Berry M. Glycan variation and evolution in the eukaryotes. Trends in Biochemical Sciences 2015; 40(7): 351- 359. doi: 10.1016/j.tibs.2015.04.004
  • 8. Pilobello KT, Slawek DE, Mahal LK. A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome. Proceedings of the National Academy of Sciences of the United States of America 2007; 104(28): 11534-11539. doi: 10.1073/pnas.0704954104
  • 9. Qin X, Guo Y, Du H, Zhong Y, Zhang J et al. Comparative analysis for glycopatterns and complex-type N-glycans of glycoprotein in sera from chronic hepatitis B- and C-infected patients. Frontiers in Physiology 2017; 8: 596. doi: 10.3389/ fphys.2017.00596
  • 10. Liang Y, Ma T, Thakur A, Yu H, Gao L et al. Differentially expressed glycosylated patterns of alpha-1-antitrypsin as serum biomarkers for the diagnosis of lung cancer. Glycobiology 2015; 25(3): 331-340. doi: 10.1093/glycob/cwu115
  • 11. Dwek RA. Glycobiology: towards understanding the function of sugars. Biochemical Society Transactions 1995; 23(1): 1-25. doi: 10.1042/bst0230001
  • 12. Plaas AH, West LA, Midura RJ. Keratan sulfate disaccharide composition determined by FACE analysis of keratanase II and endo-beta-galactosidase digestion products. Glycobiology 2001; 11(10): 779-790. doi: 10.1093/glycob/11.10.779
  • 13. Parekh RB, Dwek RA, Sutton BJ, Fernandes DL, Leung A et al. Association of rheumatoid arthritis and primary osteoarthritis with changes in the glycosylation pattern of total serum IgG. Nature 1985; 316(6027): 452-457. doi:10.1038/316452a0
  • 14. Malhotra R, Wormald MR, Rudd PM, Fischer PB, Dwek RA et al. Glycosylation changes of IgG associated with rheumatoid arthritis can activate complement via the mannose-binding protein. Nature Medicine 1995; 1(3): 237-243.
  • 15. Coelho LC, Silva PM, Lima VL, Pontual EV, Paiva PM et al. Lectins, interconnecting proteins with biotechnological/ pharmacological and therapeutic applications. EvidenceBased Complementary and Alternative Medicine 2017; 2017: 1594074. doi: 10.1155/2017/1594074
  • 16. Costa C, Brokaw JL, Wang Y, Fodor WL. Delayed rejection of porcine cartilage is averted by transgenic expression of alpha1,2-fucosyltransferase. FASEB Journal 2003; 17(1): 109- 111. doi: 10.1096/fj.02-0630fje
  • 17. Urita A, Matsuhashi T, Onodera T, Nakagawa H, Hato M et al. Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage. Arthritis & Rheumatology 2011; 63(11): 3428-3438. doi: 10.1002/art.30584
  • 18. Sethi MK, Thaysen-Andersen M, Smith JT, Baker MS, Packer NH et al. Comparative N-glycan profiling of colorectal cancer cell lines reveals unique bisecting GlcNAc and alpha-2,3- linked sialic acid determinants are associated with membrane proteins of the more metastatic/aggressive cell lines. Journal of Proteome Research 2014; 13(1): 277-288. doi: 10.1021/ pr400861m
  • 19. Zschäbitz A, Weiser H, Stofft E, Krahn V, Gabius HJ et al. Characterization of glycoconjugate expression during development of Meckel’s cartilage in the rat. Anatomy and Embryology 1995; 191(1): 47-49.
  • 20. Toegel S, Plattner VE, Wu SQ, Goldring MB, Chiari C et al. Lectin binding patterns reflect the phenotypic status of in vitro chondrocyte models. In Vitro Cellular & Developmental Biology – Animal 2009; 45(7): 351-360. doi: 10.1007/s11626- 009-9186-5
  • 21. Ishihara T, Kakiya K, Takahashi K, Miwa H, Rokushima M et al. Discovery of novel differentiation markers in the early stage of chondrogenesis by glycoform-focused reverse proteomics and genomics. Biochimica et Biophysica Acta 2014; 1840(1): 645-655. doi: 10.1016/j.bbagen.2013.10.027
  • 22. Igarashi M, Sakamoto K, Nagaoka I. Effect of glucosamine, a therapeutic agent for osteoarthritis, on osteoblastic cell differentiation. International Journal of Molecular Medicine 2011; 28(3): 373-379. doi: 10.3892/ijmm.2011.686
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Marıann RUTKA, Renáta BOR, Tamás MOLNÁR, Klaudıa FARKAS, Anna FÁBIÁN, Márk GYÖRFFY, Anıta BÁLINT, ágnes MILASSIN, Mónıka SZÜCS, László TISZLAVICZ, Ferenc NAGY, Zoltán SZEPES

Şanser GÜL, Evren AYDOĞMUŞ, Burak BAHADIR, Mustafa Çağatay BÜYÜKUYSAL, Berrak GÜVEN

The association between the route of nutrition and serum levels of adipokines in critical illness: a pilot study

Sebahattin MUHTAROĞLU, Kürşat GÜNDOĞAN, Muhammet GÜVEN, Murat SUNGUR, İsmail Hakkı AKBUDAK, Nurhayat Tuğra ÖZER, Gülşah GÜNEŞ ŞAHİN, Serap ŞAHİN, Thomas R. ZIEGLER, Ender DOĞAN

Ekrem AKSU, Ejder BERK, Abdullah SÖKMEN, Gülizar SÖKMEN, Enes ÇELİK

The usual course of thorax CT findings of COVID-19 infection and when to perform control thorax CT scan

Yakup TOMAK, Yasemin GÜNDÜZ, Mehmet Halil ÖZTÜRK

Evaluation of the cardioprotective effects of crystalloid del Nido cardioplegia solution via a rapid and accurate cardiac marker: heart-type fatty acid-binding protein

Duygun ALTINTAŞ AYKAN, Aydemir KOÇARSLAN, Adem DOĞANER, Yavuz ORAK, Mehmet KİRİŞCİ, Filiz ALKAN BAYLAN

The effect of rituximab on encapsulated peritoneal sclerosis in an experimental rat model

Süleyman KARAKÖSE, Ayşe Zeynep BAL, Eylem Pınar ESER, Murat DURANAY

Burcu Baran KETENCİOĞLU, İnsu YILMAZ, Nuri TUTAR, İnci GÜLMEZ, Fatma Sema OYMAK

Dilek DÜLGER, Özgür ALBUZ

Roles of glycoprotein glycosylation in the pathogenesis of an endemic osteoarthritis, Kashin–Beck disease, and effectiveness evaluation of sodium hyaluronate treatment

Sen WANG, Huan LIU, Peilin MENG, Cuiyan WU, Mikko J. LAMMI, Xiong GUO, Zongqiang GAO