miR-33 and RIP140 participate in LPS-induced acute lung injury

Öz Background/aim: Pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the process of acute lung injury (ALI), which can be induced by lipopolysaccharide (LPS). Numerous reports have indicated that both miR-33 and RIP140 are involved in the inflammatory response in macrophages. In this study, we sought to investigate whether miR-33 and RIP140 participate in ALI induced by LPS.Materials and methods: First, we isolated and identified PMVECs from BALB/c mice. Subsequently, both PMVECs and BALB/c mice were treated with PBS, LPS, or pyrrolidine dithiocarbamate (PDTC) plus LPS and divided into three groups: control (PBS), LPS (LPS), and L+P (LPS plus PDTC) groups. We assessed pathology by hematoxylin and eosin staining, and miR-33 and RIP140 expression levels were examined using quantitative PCR and Western blot analyses.Results: Our results demonstrated that LPS can induce PMVEC injury and ALI and that LPS treatment significantly decreased miR-33 expression compared with controls (P < 0.001, n = 5). On the contrary, RIP140 was markedly overexpressed by LPS treatment (P < 0.001, n = 5). However, this alteration can be inhibited by pretreatment with PDTC before LPS (P < 0.05, n = 5).Conclusion: This study is the first to confirm that both miR-33 and RIP140 participate in LPS-induced PMVEC injury and ALI, which may help uncover the mechanism of ALI.

Kaynak Göster

Bibtex @ { tbtkmedical577836, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {49}, pages = {422 - 428}, doi = {}, title = {miR-33 and RIP140 participate in LPS-induced acute lung injury}, key = {cite}, author = {Lı, Hua and Hou, Huan and Lıu, Shuang and Feng, Yangyang and Zhong, Wansi and Hu, Xiaojuan and Yan, Nianlong} }
APA Lı, H , Hou, H , Lıu, S , Feng, Y , Zhong, W , Hu, X , Yan, N . (2019). miR-33 and RIP140 participate in LPS-induced acute lung injury . Turkish Journal of Medical Sciences , 49 (1) , 422-428 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577836
MLA Lı, H , Hou, H , Lıu, S , Feng, Y , Zhong, W , Hu, X , Yan, N . "miR-33 and RIP140 participate in LPS-induced acute lung injury" . Turkish Journal of Medical Sciences 49 (2019 ): 422-428 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577836>
Chicago Lı, H , Hou, H , Lıu, S , Feng, Y , Zhong, W , Hu, X , Yan, N . "miR-33 and RIP140 participate in LPS-induced acute lung injury". Turkish Journal of Medical Sciences 49 (2019 ): 422-428
RIS TY - JOUR T1 - miR-33 and RIP140 participate in LPS-induced acute lung injury AU - Hua Lı , Huan Hou , Shuang Lıu , Yangyang Feng , Wansi Zhong , Xiaojuan Hu , Nianlong Yan Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 422 EP - 428 VL - 49 IS - 1 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences miR-33 and RIP140 participate in LPS-induced acute lung injury %A Hua Lı , Huan Hou , Shuang Lıu , Yangyang Feng , Wansi Zhong , Xiaojuan Hu , Nianlong Yan %T miR-33 and RIP140 participate in LPS-induced acute lung injury %D 2019 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 49 %N 1 %R %U
ISNAD Lı, Hua , Hou, Huan , Lıu, Shuang , Feng, Yangyang , Zhong, Wansi , Hu, Xiaojuan , Yan, Nianlong . "miR-33 and RIP140 participate in LPS-induced acute lung injury". Turkish Journal of Medical Sciences 49 / 1 (Şubat 2019): 422-428 .
AMA Lı H , Hou H , Lıu S , Feng Y , Zhong W , Hu X , Yan N . miR-33 and RIP140 participate in LPS-induced acute lung injury. Turkish Journal of Medical Sciences. 2019; 49(1): 422-428.
Vancouver Lı H , Hou H , Lıu S , Feng Y , Zhong W , Hu X , Yan N . miR-33 and RIP140 participate in LPS-induced acute lung injury. Turkish Journal of Medical Sciences. 2019; 49(1): 422-428.
IEEE H. Lı , H. Hou , S. Lıu , Y. Feng , W. Zhong , X. Hu ve N. Yan , "miR-33 and RIP140 participate in LPS-induced acute lung injury", Turkish Journal of Medical Sciences, c. 49, sayı. 1, ss. 422-428, Şub. 2019

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