miR-33 and RIP140 participate in LPS-induced acute lung injury

Background/aim: Pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the process of acute lung injury (ALI), which can be induced by lipopolysaccharide (LPS). Numerous reports have indicated that both miR-33 and RIP140 are involved in the inflammatory response in macrophages. In this study, we sought to investigate whether miR-33 and RIP140 participate in ALI induced by LPS.Materials and methods: First, we isolated and identified PMVECs from BALB/c mice. Subsequently, both PMVECs and BALB/c mice were treated with PBS, LPS, or pyrrolidine dithiocarbamate (PDTC) plus LPS and divided into three groups: control (PBS), LPS (LPS), and L+P (LPS plus PDTC) groups. We assessed pathology by hematoxylin and eosin staining, and miR-33 and RIP140 expression levels were examined using quantitative PCR and Western blot analyses.Results: Our results demonstrated that LPS can induce PMVEC injury and ALI and that LPS treatment significantly decreased miR-33 expression compared with controls (P < 0.001, n = 5). On the contrary, RIP140 was markedly overexpressed by LPS treatment (P < 0.001, n = 5). However, this alteration can be inhibited by pretreatment with PDTC before LPS (P < 0.05, n = 5).Conclusion: This study is the first to confirm that both miR-33 and RIP140 participate in LPS-induced PMVEC injury and ALI, which may help uncover the mechanism of ALI.
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Hasan GENÇOĞLU, Kazim ŞAHİN, Peter M. JONES

Mehmet Akif YAZAR, Mehmet Bariş AÇIKGÖZ, Adnan BAYRAM

Prevalence of multiple sclerosis in an urban population of Sivas province in Turkey

Aslı BOLAYIR, Özlem KAYIM YILDIZ, Hatice BALABAN, Şeyda Figül GÖKÇE, Sanem NEMMEZİ KARACA, Ahmet Suat TOPAKTAŞ, Burhanettin ÇİĞDEM

Virulence factors and antimicrobial resistance in uropathogenic Escherichia coli strains isolated from cystitis and pyelonephritis

Mansour SEDIGHI, Shiva MIRKALANTARI, Keyvan TAHERI, Elahe EDALATI, Hosseinali GHAZVINI

Effects of Ankaferd Hemostat on Helicobacter pylori strains and antibiotic resistance

Rafiye ÇİFTÇİLER, Nejat AKAR, Ahmet KOLUMAN, İbrahim C. HAZNEDAROĞLU

Canan Albayrak YAŞAR, Pinar TARKUN, Elif Birtaş ATEŞOĞLU, Fatma Ceyla ERALDEMİR, Özgür Doğa ÖZSOY, Esra Terzi DEMİRSOY, Özgür MEHTAP, Ayfer GEDÜK, Abdullah HACİHANİFİOĞLU

A comparative biomechanical analysis of suprapectineal and infrapectineal fixation on acetabular anterior column fracture by finite element modeling

Ahmet Özgür YILDIRIM, Mehmet YÜCENS, Nevres H. AYDOĞAN, Kadir Bahadır ALEMDAROĞLU, Serkan İLTAR, Ahmet ÖZMERİÇ

Factors associated with glomerular filtration rate variation in primary hyperparathyroidism after parathyroidectomy

Fatih AKCAN, Mümtaz TAKIR, Selvihan BEYSEL, Muhammed KIZILGÜL, Bekir UÇAN, Mustafa ÇALIŞKAN, Erman ÇAKAL, Mustafa ÖZBEK

About “Efficacy of bispectral index monitoring for prevention of anesthetic awareness and complications during oocyte pick-up procedure

Ayşe ÇİĞDEM TÜTÜNCÜ

Comparison of early and long term outcomes of open Lichtenstein repair and totally extraperitoneal herniorrhaphy for primary inguinal hernias

Ömer KARAHAN, Nurullah DAMBURACI, Murat GÜNER, Barış SEVİNÇ