Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum. Materials and methods: An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism. Results: The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation were higher than those before biofilm formation. The expression of the metabolism-related gene ureC in postbiofilm formation was higher than those in prebiofilm formation. Conclusion: It seems that biofilm formation is quite important in the generation of quinolone resistance in Ureaplasma. It is very useful to detect biofilm formation activity as well as to analyze other laboratory parameters for Ureaplasma.

Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum. Materials and methods: An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism. Results: The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation were higher than those before biofilm formation. The expression of the metabolism-related gene ureC in postbiofilm formation was higher than those in prebiofilm formation. Conclusion: It seems that biofilm formation is quite important in the generation of quinolone resistance in Ureaplasma. It is very useful to detect biofilm formation activity as well as to analyze other laboratory parameters for Ureaplasma.

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Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

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Protective effects of royal jelly against testicular damage in streptozotocin-induced diabetic rats

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An alternative marker of low-density lipoprotein cholesterol in coronary artery disease: non-high-density lipoprotein cholesterol

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An in vitro and in vivo investigation of the cytotoxic effects of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester and bortezomib in multiple myeloma cells

Ertan ALTAYLI, Özgür KORU, Önder ÖNGÖRÜ, Tayfun İDE, Cengizhan AÇIKEL, Meral SARPER, Mualla Pınar ELÇİ, Rahşan ILIKÇI SAĞKAN, Erhan ASTARCI, Duran TOK, Salim ÖZENÇ, Ali Uğur URAL, Ferit AVCU

Aberrant expression of leukemia inhibitory factor receptor (LIFR) and leukemia inhibitory factor (LIF) is associated with tubal pregnancy occurrence

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Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates

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The effect of total intravenous anesthesia on the postoperative cognitive functions of young and elderly patients after lumbar disk surgery

Gizem İLVAN, Hatice Zerrin ÖZKÖSE