Meryem ÇOLAK, Gülendam BOZDAYI, Aylin ALTAY, Zahide YALAKİ, Kamruddin AHMED, Seçil ÖZKAN

Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea

Öz Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons. Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method. Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing. Conclusion: AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.

Anahtar Kelimeler:

Adenovirus, children, PCR, gastroenteritis, genotype, diarrhoea, phylogenetic analysis

Tam Metin

Kaynak Göster

Bibtex	`@ { tbtkmedical576375, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2017}, volume = {47}, pages = {1463 - 1471}, doi = {}, title = {Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea}, key = {cite}, author = {Çolak, Meryem and Bozdayı, Gülendam and Altay, Aylin and Yalaki, Zahide and Ahmed, Kamruddin and Özkan, Seçil} }`
APA	Çolak, M , Bozdayı, G , Altay, A , Yalaki, Z , Ahmed, K , Özkan, S . (2017). Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea . Turkish Journal of Medical Sciences , 47 (5) , 1463-1471 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45689/576375
MLA	Çolak, M , Bozdayı, G , Altay, A , Yalaki, Z , Ahmed, K , Özkan, S . "Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea" . Turkish Journal of Medical Sciences 47 (2017 ): 1463-1471 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45689/576375>
Chicago	Çolak, M , Bozdayı, G , Altay, A , Yalaki, Z , Ahmed, K , Özkan, S . "Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea". Turkish Journal of Medical Sciences 47 (2017 ): 1463-1471
RIS	TY - JOUR T1 - Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea AU - Meryem Çolak , Gülendam Bozdayı , Aylin Altay , Zahide Yalaki , Kamruddin Ahmed , Seçil Özkan Y1 - 2017 PY - 2017 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1463 EP - 1471 VL - 47 IS - 5 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Medical Sciences Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea %A Meryem Çolak , Gülendam Bozdayı , Aylin Altay , Zahide Yalaki , Kamruddin Ahmed , Seçil Özkan %T Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea %D 2017 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 47 %N 5 %R %U
ISNAD	Çolak, Meryem , Bozdayı, Gülendam , Altay, Aylin , Yalaki, Zahide , Ahmed, Kamruddin , Özkan, Seçil . "Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea". Turkish Journal of Medical Sciences 47 / 5 (Ekim 2017): 1463-1471 .
AMA	Çolak M , Bozdayı G , Altay A , Yalaki Z , Ahmed K , Özkan S . Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea. Turkish Journal of Medical Sciences. 2017; 47(5): 1463-1471.
Vancouver	Çolak M , Bozdayı G , Altay A , Yalaki Z , Ahmed K , Özkan S . Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea. Turkish Journal of Medical Sciences. 2017; 47(5): 1463-1471.
IEEE	M. Çolak , G. Bozdayı , A. Altay , Z. Yalaki , K. Ahmed ve S. Özkan , "Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea", Turkish Journal of Medical Sciences, c. 47, sayı. 5, ss. 1463-1471, Eki. 2017