Hamza ÖZAVCI, Mustafa KAPLAN

Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate

Öz Background/aim: Thiol-specific antioxidant (TSA) protein is one of the most promising molecules among candidates for vaccine against cutaneous leishmaniasis. It was found to be significantly protective against different Leishmania species. In this study, cloning and molecular characterization of thiol-specific antioxidant gene of L. tropica Turkey isolate (LtTSA) were aimed. Materials and methods: LtTSA was amplified by PCR using the specific primers of TSA gene and cloned into the pcDNA3.1 vector. The cloning was confirmed by PCR screening, restriction enzyme reactions, and DNA sequence analysis. Finally, three-dimensional structure and antigenic properties of the protein encoded by the LtTSA were determined.Results: Six hundred base pair bands belonging to LtTSA were shown with electrophoresis. It was found that LtTSA and its encoded protein have high similarity with different Leishmania species. LtTSA protein consisting of 199 amino acids was found to have 7 different antigenic regions. Conclusion: LtTSA and its encoded TSA protein were found to be highly immunogenic and similar to TSA proteins previously tested as a vaccine candidate.

Anahtar Kelimeler:

Cutaneous leishmaniasis, Thiol-specific antioxidant, cloning, DNA vaccine

Tam Metin

Kaynak Göster

Bibtex	`@ { tbtkmedical577833, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {49}, pages = {392 - 402}, doi = {}, title = {Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate}, key = {cite}, author = {Özavcı, Hamza and Kaplan, Mustafa} }`
APA	Özavcı, H , Kaplan, M . (2019). Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate . Turkish Journal of Medical Sciences , 49 (1) , 392-402 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577833
MLA	Özavcı, H , Kaplan, M . "Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate" . Turkish Journal of Medical Sciences 49 (2019 ): 392-402 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45750/577833>
Chicago	Özavcı, H , Kaplan, M . "Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate". Turkish Journal of Medical Sciences 49 (2019 ): 392-402
RIS	TY - JOUR T1 - Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate AU - Hamza Özavcı , Mustafa Kaplan Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 392 EP - 402 VL - 49 IS - 1 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Medical Sciences Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate %A Hamza Özavcı , Mustafa Kaplan %T Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate %D 2019 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 49 %N 1 %R %U
ISNAD	Özavcı, Hamza , Kaplan, Mustafa . "Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate". Turkish Journal of Medical Sciences 49 / 1 (Şubat 2019): 392-402 .
AMA	Özavcı H , Kaplan M . Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate. Turkish Journal of Medical Sciences. 2019; 49(1): 392-402.
Vancouver	Özavcı H , Kaplan M . Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate. Turkish Journal of Medical Sciences. 2019; 49(1): 392-402.
IEEE	H. Özavcı ve M. Kaplan , "Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate", Turkish Journal of Medical Sciences, c. 49, sayı. 1, ss. 392-402, Şub. 2019