Betül DEMİRCİ, Gülden Koltuksuz YASDIKCIOĞLU, Kemal Hüsnü Can BAŞER

Sesquiterpene hydrocarbons of the essential oil of Actinolema macrolema Boiss

The essential oils of Actinolema macrolema Boiss. (Apiaceae) were obtained by hydrodistillation in the first 3 (sample A) and the following 3 h (sample B) from crushed fruits and dried leaves (sample C), which were subsequently analyzed by GC and GC-MS. Overall, 64 components were characterized, representing 93% of the leaf oil. Thirty components were characterized from the fruit oil representing 95% of the first fraction and 90% of the second fraction consecutively. Guaia-5,7(11)-diene, selina-3,7(11)-diene, and juniper camphor were isolated from the oils by column chromatography and their structures were elucidated by GC-MS, 1H NMR, and 13C NMR. The occurrence of guaia-5,7(11)-diene in nature is reported for the first time. Guaia-5,7(11)-diene (37% and 30%), germacrene-B (25% and 21%), and selina-3,7(11)-diene (both 12%) were found as major components in the oil of sample A and the following sample B, respectively. In sample C, 1-octadecanol (24%) and hexadecanoic acid (19%) were identified as the major components. Additionally, antimicrobial activities of the fruit oils were determined using broth microdilution. Sample A exhibited relatively good inhibition of Staphylococcus epidermidis (MIC 62.5 m g/mL). The 2 fruit essential oils showed inhibitory (MIC 125 m g/mL) effects equal to those of the standard antifungal agent used against C. albicans.

Anahtar Kelimeler:

Actinolema macrolema, Apiaceae, essential oil, isolation, guaia-5, 7(11)-diene, GC-MS, biological activity

Sesquiterpene hydrocarbons of the essential oil of Actinolema macrolema Boiss

Keywords:

Actinolema macrolema, Apiaceae, essential oil, isolation, guaia-5, 7(11)-diene, GC-MS, biological activity,

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