İsolation And İdentification Of A New Ac TO

Isolation and identification of a new acetildenafil analogue used to adulterate a dietary supplement: dimethylacetildenafil

A new sildenafil analogue was found to have been added illegally to a dietary supplement marketed for the enhancement of sexual function. The structure was determined as 5-(2-ethoxy-5- {[4-(3,5-dimethyl)piperazin-1-yl]-acetyl}phenyl)-1,6-dihydro-1-methyl-3- propyl-7H-pyrazolo [4,3-d]pyrimidin-7-one. Owing to the inclusion of a dimethyl group in acetildenafil, the detected compound was called dimethylacetildenafil. The sample was purified with column chromatography. The IR, LC/MS (ESI), and completely assigned NMR data of dimethylacetildenafil are reported for the first time. The structure was compared with that of acetildenafil and the results showed that the ethyl substitution at the 4-position of the piperazine ring had been replaced by a 3,5-dimethyl substitution. This new acetildenafil analogue was isolated and identified for the first time.

Anahtar Kelimeler:

Acetildenafil analogue, dimethylacetildenafil, adulteration, NMR, electrospray ionization (ESI), herbal aphrodisiac.

Isolation and identification of a new acetildenafil analogue used to adulterate a dietary supplement: dimethylacetildenafil

Keywords:

Acetildenafil analogue, dimethylacetildenafil, adulteration, NMR, electrospray ionization (ESI), herbal aphrodisiac.,

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Based on the mass, UV, IR spectrum, and NMR spectroscopic data, the structure of compound 1 was determined as 5-(2-ethoxy-5-{[4-(3,5-dimethyl)piperazine-1-yl]-acetyl}phenyl)-1,6-dihydro-1-methyl-3-propyl-7H−pyrazolo[4,3
d ]pyrimidin-7-on, a new acetildenaŞl analogue (Figure 1), which, when compared with acetildenaŞl, showed that the N-ethylpiperazine was switched with an 3,5-dimethylpiperazine. This compound must be included in the inspection list for illegal health-related substances because of the unknown safety and toxicity proŞle.