Development and Characterization of a Cationic Emulsion Formulation as a Potential pDNA Carrier System

The development of efficient and stable carriers for the delivery of DNA to the body is becoming an increasingly important issue in the fields of gene therapy and vaccination. The present study was designed to prepare an emulsion-based gene delivery system. Oil-in-water emulsions containing cetyltrimethylammonium bromide (CTAB) as a cationic surfactant and Pluronic F-68 as a nonionic co-surfactant were formulated and their physical characteristics were investigated. The cationic emulsion, referred to as B13d, containing a liquid crystal phase, was found to be stable and suitable for parenteral application. Complex formation between pGL3 plasmid DNA and B13d emulsion achieved at the pDNA:CTAB ratio of 1:2.74 (m g/nmol) was demonstrated using the gel retardation and SYBR Green I displacement assays. Particle sizes of the freshly prepared empty and pDNA-associated B13d emulsions were about 350 nm. Zeta potentials of empty and pDNA loaded emulsions were +45.3 mV and +43.7 mV, respectively. The sensitivity of pDNA/emulsion complexes to endonuclease digestion was determined and complexes were found to be highly effective for protecting pDNA from DNase I attack. The final in vitro stability analysis carried out in the presence of human serum revealed that the super-coiled pDNA was observable even after 24 h of incubation. The physical characteristics and serum resistant properties of the complexes suggest that B13d emulsion could be an efficient pDNA carrier system for gene and/or immunogene delivery.

Development and Characterization of a Cationic Emulsion Formulation as a Potential pDNA Carrier System

The development of efficient and stable carriers for the delivery of DNA to the body is becoming an increasingly important issue in the fields of gene therapy and vaccination. The present study was designed to prepare an emulsion-based gene delivery system. Oil-in-water emulsions containing cetyltrimethylammonium bromide (CTAB) as a cationic surfactant and Pluronic F-68 as a nonionic co-surfactant were formulated and their physical characteristics were investigated. The cationic emulsion, referred to as B13d, containing a liquid crystal phase, was found to be stable and suitable for parenteral application. Complex formation between pGL3 plasmid DNA and B13d emulsion achieved at the pDNA:CTAB ratio of 1:2.74 (m g/nmol) was demonstrated using the gel retardation and SYBR Green I displacement assays. Particle sizes of the freshly prepared empty and pDNA-associated B13d emulsions were about 350 nm. Zeta potentials of empty and pDNA loaded emulsions were +45.3 mV and +43.7 mV, respectively. The sensitivity of pDNA/emulsion complexes to endonuclease digestion was determined and complexes were found to be highly effective for protecting pDNA from DNase I attack. The final in vitro stability analysis carried out in the presence of human serum revealed that the super-coiled pDNA was observable even after 24 h of incubation. The physical characteristics and serum resistant properties of the complexes suggest that B13d emulsion could be an efficient pDNA carrier system for gene and/or immunogene delivery.

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Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Determination of Trace Copper by FAAS after Solid Phase Extraction and Preconcentration onto Amberlite XAD-2 Loaded with Nitroso-R Salt

Mohammad Ali TAHER, Sayed Ziae Mohammadi

Crystal Structure of the cis-bis[4-chloro-N-(pyrrolidine-1-carbothioyl)-benzamido] copper(II) Complex

Nevzat KÜLCÜ, Ulrich FLÖRKE, Hakan ARSLAN

The Synthesis and Applications of Cr-Doped GLYMO and GLYMO/DAMO Surface Coating Systems for Absorbing UV-light by Sol-Gel Process

Meltem ASİLTÜRK, Funda SAYILKAN, Galip İÇDUYGU

Development and Characterization of a Cationic Emulsion Formulation as a Potential pDNA Carrier System

Kudret Dilşad BARUT, Fatma Filiz COŞKUN ARI, Filiz ÖNER

Cobalt Impregnated Alumina: Nitrogen Adsorption Study at 77 K

Riaz QADEER, Sumeera IKRAM

Potentiometric studies on the protonation constants and solvation of some$alpha$-amino acid benzyl-and t-butyl-esters in ethanol-water mixtures

Esma KILIÇ, Alev DOĞAN

Secondary Metabolites of Phlomis viscosa and Their Biological Activities

İhsan ÇALIŞ, Hasan KIRMIZIBEKMEZ, John A. BEUTLER, Ali A. DÖNMEZ, Funda Nuray YALÇIN, Ekrem KILIÇ, Meral ÖZALP, Peter RÜEDI, Deniz TAŞDEMİR

Synthesis and Complex Formation of Some Novel vic-Dioxime Derivatives of Hydrazones

Nursabah SARIKAVAKLI, Gazi İREZ

Synthesis and Characterization of Novel Hyperbranched Polyimides Based on Silsesquioxane Nanocomposite Networks

Turgay SEÇKİN, Ahmet GÜLTEK, Süleyman KÖYTEPE

Diels-Alder Reactions of New Methoxysubstituted-6-arylfulvenes

Nüket ÖCAL, Emine BAĞDATLI, Mesut ARSLAN