Ufuk KOLAK, Işıl HACIBEKİROĞLU, Mehmet ÖZTÜRK, Fevzi ÖZGÖKÇE, Gülaçtı TOPÇU, Ayhan ULUBELEN

Antioxidant and anticholinesterase constituents of Salvia poculata

Two triterpenoids, namely 2a,3a-dihydroxy-24-nor- 4(23),12-oleanadien-28-oic acid (1) and ursolic acid (2); 4 flavonoids, namely 5-hydroxy-7,4'-dimethoxyflavone (3), cirsimaritin (4), eupatilin (5), and salvigenin (6); a diterpenoid, namely sclareol (7); and a steroid, namely b-sitosterol (8), were isolated from the aerial parts of Salvia poculata Nab., a Turkish endemic Salvia species. Total phenolic and flavonoid contents of the crude extract were determined as pyrocatechol and quercetin equivalents, respectively. The antioxidant activity of the crude extract and the isolated compounds (2-8) was established using b-carotene bleaching, superoxide anion radical, and ABTS cation radical scavenging activity. In addition, the anticholinesterase activity of the crude extract and the isolated compounds (2-8) against the enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was determined. The phytochemistry and antioxidant and anticholinesterase activities of S. poculata were investigated for the first time in this study. The crude extract of S. poculata exhibited a significant antioxidant effect as well as butyrylcholinesterase inhibitory activity. Ursolic acid (2) and cirsimaritin (4) possessed high butyrylcholinesterase inhibitory activity.

Anahtar Kelimeler:

Salvia poculata; Lamiaceae; antioxidant activity; anticholinesterase activity.

Antioxidant and anticholinesterase constituents of Salvia poculata

Keywords:

Salvia poculata; Lamiaceae; antioxidant activity; anticholinesterase activity.,

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the Turkish Academy of Sciences (TUBA) for the partial support of this study. Reische, D. W.; Lillard, D. A.; Eitenmiller, R. R. Antioxidants. In Food Lipids (Chemistry, nutrition and biotech- nology). Akoh, C. C.; Min, D. B., Eds.; pp. 489-516: Marcel Dekker, New York, 2002.
Fraga, B. M.; Diaz, C. E.; Guadano, A.; Gonzalez-Coloma, A. J. Agric. Food Chem. 2005, textit53, 5200-5206.
Mukherjee, P. K.; Kumar, V.; Mal, M.; Houghton, P. J. Phytomedicine 2007, textit14, 289-300.
Howes, M-J. R.; Perry, N. S. L.; Houghton, P. J. Phytother. Res. 2003, textit17, 1-18.
Scholey, A. B.; Tildesley, N. T. J.; Ballard, C. G.; Wesnes, K. A.; Tasker, A.; Perry, E. K.; Kennedy, D. O. Psychopharmacology 2008, textit198, 127-139.
Top¸cu, G. J. Nat. Prod. 2006, textit69, 482-487.
Savelev, S. U.; Okello, E. J.; Perry, E. K. Phytother. Res. 2004, textit18, 315-324.
Delamare, A. P. L.; Moschen-Pistorello, I. T.; Artico, L.; Atti-SeraŞni, L.; Echeverrigaray, S. Food Chem. 2007, textit100, 603-608.
Hedge, I. C. Salvia L. In Flora of Turkey and the East Aegean Islands; Davis, P.H., Ed.; vol. 7, p. 400-459, Edinburgh University Press, Edinburgh, 1982.
Baytop, T. Therapy with Medicinal Plants in Turkey, pp. 156-158: Istanbul University Press, ˙Istanbul, 1984.
Ulubelen, A.; Top¸cu. G. Chemical and Biological Investigations of Salvia species growing in Turkey. In Studies in Natural Products Chemistry; Atta-ur-Rahman, Ed.; vol. 20, pp. 659-718, Elsevier Press, New York, 1998.
G¨oren, A. C.; Kılı¸c, T.; Dirmenci, T.; Bilsel, G. Biochem. Syst. Ecol. 2006, textit34, 160-164.
Ballesta-Acosta, M. C.; Pascual-Villalobos, M. J.; Rodriguez, B. J. Nat. Prod. 2002, textit65, 1513-1515.
Ogura, M.; Cordell, G. A.; Farnsworth, N. R. Lloydia 1977, textit40, 157-168.
Ahmad, S. Planta Med. 1983,textit 48, 62-63.
Mues, R.; Timmermann, B. N.; Ohno, N.; Mabry, T. J. Phytochemistry 1979, textit18, 1379-1383.
Appendino, G.; Valle, M. G.; Nano, G. M. Fitoterapia 1987, LVIII, 115-118.
Ulubelen, A.; Ozturk, S.; Isıldatıcı, S. J. Pharm. Sci. 1968, textit57, 1037-1038.
Ulubelen, A.; Top¸cu. G.; Eris, C.; S¨onmez, U.; Kartal, M.; Kurucu, S.; Bozok-Johansson, C. Phytochemistry 1994, textit36, 971-974.
DellaGreca, M. D.; Monaco, P.; Previtera, L. J. Nat. Prod. 1990, textit53, 1430-1435.
Slinkard, K.; Singleton, V. L. Am. J. Enol. Viticult. 1977, textit28, 49-55.
Moreno, M. I. N.; Isla, M. I.; Sampietro, A. R.; Vattuone, M. A. J. Ethnopharmacol. 2000, textit71, 109-114.
Miller, H. M. J. Am. Oil Chem. Soc. 1971, textit45, 91.
Liu, F.; Ooi, V. E. C.; Chang, S. T. Life Sci. 1997, textit60, 763-771.
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Free Radical Bio. Med. 1999, textit26, 1231-1237.
Ellman, G. L.; Courtney K. D.; Andres V.; Featherston, R. M. Biochem. Pharmacol. 1961, textit7, 88-95.
D’Abrosca, B.; Fiorentino, A.; Monaco, P.; Oriano, P.; PaciŞco, S. Food Chem. 2006,textit 98, 285-290.
Yin, M-C; Chan, K-C. J. Agr. Food Chem. 2007, textit55, 7177-7181.
Martini, N. D.; Katerere, D. R. P.; Eloﬀ, J. N. J. Ethnopharmacol. 2004, textit93, 207-212.
Ono, M.; Morinaga, H.; Masuoka, C.; Ikeda, T.; Okawa, M.; Kinjo, J.; Nohara, T. Chem. Pharm. Bul. 2005, textit53, 1175-1177.
Zheng, W.; Wang, S. Y. J. Agr. Food Chem. 2001, textit49, 5165-5170.
Ibanez, E.; Kubatova, A.; Senorans, F. J.; Cavero, S.; Reglero, G.; Hawthorne, S. B. J. Agr. Food Chem. 2003,textit , 375-382.
Han, J.; Weng, X.; Bi, K. Food Chem. 2008, textit106, 2-10.
Weng, X. C.; Wang, W. Food Chem. 2000, textit71, 489-493.
Chung, Y. K.; Heo, H. J.; Kim, E. K.; Kim, H. K.; Huh, T. L.; Lim, Y.; Kim, S. K.; Shin, D. H. Mol. Cells 2001, textit11, 137-143.