Anti-Influenza Virus Activity of Water Soluble Extracts of Sanicula europaeaL. Rhizomes

The effects of the Sanicula europaea L. rhizome extracts on influenza virus replication in MDCK cells were investigated. Four different fractions (Fr.I R , Fr.II R , Fr. III R , Fr. IV R ) were obtained from the crude extract of Sanicula europaea L. rhizomes through Sephadex G-100 gel filtration col-umn chromatography. Fr.I R , Fr.II R , and Fr. III R , were found to be non-toxic to MDCK cells up to a con-centration of 100 ?g/ml. However, Fr. IV R was found to be slightly toxic at a concentration of 100 ?g/ml. The antriviral activity of non-toxic fractions (Fr.I R , Fr.II R , and Fr. III R ) against influenza virus-es was tested by plaque inhibition test. The replication of influenza A/PR/8/34 viruses was inhibited by these fractions. The highest activity against influenza A/PR/8/34 was found in Fr.II R ..Influenza A/Vic/1/75 produced microscopic plaques in the presence of the extract. However, the same fractions were not effective against influenza B/Lee/40. These observations show that the Sanicula rhizome extract contains an anti-influenza A virus substance.

Anti-Influenza Virus Activity of Water Soluble Extracts of Sanicula europaeaL. Rhizomes

The effects of the Sanicula europaea L. rhizome extracts on influenza virus replication in MDCK cells were investigated. Four different fractions (Fr.I R , Fr.II R , Fr. III R , Fr. IV R ) were obtained from the crude extract of Sanicula europaea L. rhizomes through Sephadex G-100 gel filtration col-umn chromatography. Fr.I R , Fr.II R , and Fr. III R , were found to be non-toxic to MDCK cells up to a con-centration of 100 ?g/ml. However, Fr. IV R was found to be slightly toxic at a concentration of 100 ?g/ml. The antriviral activity of non-toxic fractions (Fr.I R , Fr.II R , and Fr. III R ) against influenza virus-es was tested by plaque inhibition test. The replication of influenza A/PR/8/34 viruses was inhibited by these fractions. The highest activity against influenza A/PR/8/34 was found in Fr.II R ..Influenza A/Vic/1/75 produced microscopic plaques in the presence of the extract. However, the same fractions were not effective against influenza B/Lee/40. These observations show that the Sanicula rhizome extract contains an anti-influenza A virus substance.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK