Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L. ‘Badami’) seedlings
The mechanism(s) by which Zn alleviates NaCl stress was investigated in Pistacia vera L. 'Badami'. Pistachio seedlings were treated with 0, 800, 1600, 2400, and 3200 mg of NaCl kg-1 of soil, along with Zn (0, 5, 10, and 20 mg kg-1 of soil). NaCl stress induced high oxidative stress, increasing lipid peroxidation, electrolyte leakage, and lipoxygenase (LOX, E.C. 1.13.11.12) activity to high levels. Zn supplement efficiently reduced all these adverse effects of salt stress. However, there were no significant differences among the above parameters in plants treated with Zn only. NaCl stress significantly increased the activity of antioxidant enzymes. The activity of antioxidant enzymes (superoxide dismutase [SOD, E.C. 1.15.1.1], catalase [CAT, E.C. 1.11.1.6]. and ascorbate peroxidase [APX, E.C. 1.11.1.11]) increased significantly in NaCl + Zn-treated plants, as compared with those treated with either NaCl or Zn alone. These results support the positive effects of Zn on antioxidant enzyme activity scavenging the reactive oxygen species produced in response to salt stress. Zinc treatment caused a significant increase in the amount of phenolics and in ascorbic acid content, as compared with salt treatment alone. Zinc treatment alone did not result in very high antioxidant enzyme activity in non-stressed plants.
Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L. ‘Badami’) seedlings
The mechanism(s) by which Zn alleviates NaCl stress was investigated in Pistacia vera L. 'Badami'. Pistachio seedlings were treated with 0, 800, 1600, 2400, and 3200 mg of NaCl kg-1 of soil, along with Zn (0, 5, 10, and 20 mg kg-1 of soil). NaCl stress induced high oxidative stress, increasing lipid peroxidation, electrolyte leakage, and lipoxygenase (LOX, E.C. 1.13.11.12) activity to high levels. Zn supplement efficiently reduced all these adverse effects of salt stress. However, there were no significant differences among the above parameters in plants treated with Zn only. NaCl stress significantly increased the activity of antioxidant enzymes. The activity of antioxidant enzymes (superoxide dismutase [SOD, E.C. 1.15.1.1], catalase [CAT, E.C. 1.11.1.6]. and ascorbate peroxidase [APX, E.C. 1.11.1.11]) increased significantly in NaCl + Zn-treated plants, as compared with those treated with either NaCl or Zn alone. These results support the positive effects of Zn on antioxidant enzyme activity scavenging the reactive oxygen species produced in response to salt stress. Zinc treatment caused a significant increase in the amount of phenolics and in ascorbic acid content, as compared with salt treatment alone. Zinc treatment alone did not result in very high antioxidant enzyme activity in non-stressed plants.
Keywords:
Key words: Antioxidant enzymes, pistachio, salt stress, zinc effects,
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Abou Hossein EA, Shehata MM, El-Sherif MA (2002) Phosphorus nutrition of barley plant as affected by zinc, manganese and organic matter application to saline soils. Egypt J Soil Sci 42: 331-345.Alpaslan M, Inal A, Gunes A, Cikil Y, Ozcan H (1999) Effect of zinc treatment on the alleviation of sodium and chloride injury in tomato (Lycopersicon esculentum L.) grown under salinity. Turk J Agric For 23: 1-6.
AOAC (1990) Official Methods of Analysis of the Association of Official Analytical Chemists. 15thed. Helrich, K. ed.; AOAC: Arlington, VA p. 703.
Aslam M, Ranjha AM, Akhtar J (2000) Salinity tolerance of rice affected by zinc application. Pak J Biol Sci 3: 2055-2057.
Becana M, Dalton DA, Moran JF, Iturbe OI, Matamoros MA, Rubio MC (2000) Reactive oxygen species and antioxidants in legume nodules. Physiol Plant 109: 372-381.
Bettger, WJ, O’Dell BL (1981) A critical physiological role of zinc in the structure and function of biomembranes. Life Sci 28: 1425- 1438.
Bray, TM, Bettger WJ (1990) The physiological role of zinc as an antioxidant. Free Rad Biol Med 8: 281-291.
Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146: 185-205.
Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascrobate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98: 1222-1227.
Cakmak I, Marschner H (1988a) Enhanced superoxide radical production in roots of zinc-deficient plants. J Exp Bot 39: 1449- 1460.
Cakmak I, Marschner H (1988b) Zinc-dependent changes in ESR signals, NADPH oxidase and plasma membrane permeabilityin cotton roots. Physiol Plant 73: 182-186.
Casano LM, Gomez LD, Lascano HR, Gonzalez CA, Trippi VS (1997) Inactivation and degradation of Cu/Zn SOD by active oxygen species in wheat chloroplasts exposed to photooxidative stress. Plant Cell Physiol 38: 433-440.
Chvapil M (1973) New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes. Life Sci 13: 1041-1049.
Dionisio-Sesc LM, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135: 1-9.
Ederli, L, Pasqualini S Batini P, Antonielli M (1997) Photoinhibition and oxidative stress: effects on xanthophyll cycle, scavenger enzymes and abscissic acid content in tobacco plants. J Plant Physiol 151: 422-428.
Foyer CH, Noctor G (2000) Oxygen processing in photosynthesis: Regulation and signaling. New Phytol 146: 359-388.
Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plant 92: 696-717.
Fridovich I (1986) Biological effects of the superoxide radical. Arch Biochem Biophys 247: 1-11.
Giannopotitis CN, Ries SK (1977) Superoxide dismutase in higher plants. Plant Physiol 59: 309-314.
Heath RL, Parker L (1968) Photoperoxidation in isolated chloroplast. 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189-198.
Kanazawa S, Sano S, Koshiba T, Ushimaru T (2000) Changes in antioxidative in cucumber cotyledons during natural senescence: comparison with those during dark-induced senescence. Physiol Plant 109: 211-216.
Kawano T, Kawano N, Muto S, Lapeyrie F (2001) Cation-induced superoxide generation in tobacco cell suspension culture is dependent on ion valence. Plant Cell Environ 24: 1235-1241.
Lacan D, Baccou JC (1998) High levels of antioxidant enzymes correlate with delayed senescence in non-netted muskmelon fruits. Planta 204: 377-382.
Lin CC, Kao CH (2000) Effect of NaCl stress on H2O2metabolism in rice leaves. Plant Growth Regul 30: 151-155.
Logani MK, Davies RE (1980) Lipid oxidation: biologic effects and antioxidants - a review. Lipids 15: 485-495.
Lowry OH, Rosenberg NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 265- 275.
Marschner H, Cakmak I (1986) High light intensity enhances chlorosis and necrosis in leaves of Zn-, potassium- and magnesium-deficient bean (Phaseolus vulgaris) plants. J Plant Physiol 134: 308-315.
Mukherjee SP, Choudhuri MA (1985) Implication of hydrogen peroxide-ascorbate system on membrane permeability of water stressed Vigna seedlings. New Phytol 99: 355-360.
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinch chloroplasts. Plant Cell Physiol 22: 867-880.
Noctor G, Foyer C (1998) Ascorbate and glutathione: keeping active oxygen under control, Annu. Rev. Plant Physiol. Plant Mol Biol 49: 249-279.
Olmos E, Hemandez JA, sevilla F, Hellin E (1994) Induction of several antioxidant enzymes in the selection of a salt-tolerant cell line of Pisum sativum. J Plant Physiol 144: 594-598.
Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Exotoxicol Environ Safe 60: 324-349.
Parker DR, Aguilera JJ, Thomson DN (1992) Zinc-Phosphorus interactions in two cultivars of tomato (Lycopersicon esculentum L.) grown in chelator-buffered nutrient solutions. Plant Soil 143: 163-177.
Rice-Evans AC, Miller NJ, Pagaga G (1997) Antioxidant properties of phenolic compounds. New Trends Plant Sci Rev 2: 152-159.
Sairam RK, Deshmukb PS, Shukla DS (1997) Tolerance to drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. J Agron Crop Sci 178: 171-177.
Shalata A, Tal M (1998) The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol Plant 104: 167-174.
Sharma PN, Kumar N, Bisht SS (1994) Effect of zinc deficiency on chlorophyll content, photosynthesis and water relations of cauliflower plants. Photosynthetica 30: 353-359.
Vallee BL, Auld DS (1990) Zinc coordination, function and structure of zinc enzymes and other proteins. Biochemistry 29: 5647- 5659.
Vallee BL, Falchuk KH (1993) The biochemical basis of zinc
physiology. Phys Rev 73: 79-118.
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant system in acid rain treated bean plants: protective role of exogenous polyamines. Plant Sci 151: 59-66.
Victória AP, Lea PJ, Azevedo RA (2001) Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry 57: 710-710.
Zago MP, Oteiza PI (2001) The antioxidant properties of zinc: Interactions with iron and antioxidants. Free Rad Biol Med 31: 266-274.
Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6: 441-445.
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