Aynur Çolak TÜMBEK, Pınar ÖZEREN, Rıza KAYA, Yakup Zekai KATIRCIOĞLU, Salih MADEN

Türkiye’de Cercospora beticola popülasyonlarının *utriafol, mancozeb ve fentin acetate’a duyarlılıkları

Cercospora beticola’ nın neden olduğu Şeker Pancarı Yaprak Lekesi hastalığının Türkiye’ de kullanılan fungisitlere karşı dayanıklılığı petri kaplarında mycelial gelişme deneyleri ile 2005-2006 yıllarında 6 şeker pancarı fabrikası üretim alanından elde edilen 100 fungus izolatı kullanılarak araştırılmıştır. Bunun için flutriafol, mancozeb ve fentin asetat Aspergillus Complete Medium (ACM)’ a 0.0, 0.05, 0.1, 0.5, 1.0, 5.0, 10.0 µg mL-1 dozlarda katılmış ve miselyal gelişme ölçülmüştür. Şeker pancarı üretim alanlarından elde edilen tüm izolatların flutriafol için ED50 değerleri 0.04 ile > 10.0 µg mL-1 arasında değişmiştir. Bu fungiside karşı tolerans, bu hastalığın gelişmesi için uygun iklim koşullarını içeren ve hastalığın şiddetli olduğu iki bölgede, Adapazarı ve Çarşamba, belirgin derecede yüksek bulunmuştur. Bu iki bölgede; izolatların sırasıyla %74.1, %55.5, %81.2 ve %68.7’ i, fungusun gelişmesini engellediği kabul edilen 5 ve 10 µg mL-1 flutriafol dozunda gelişmiştir. Diğer 4 bölgede, bu fungisite tolerans bu kadar yüksek oranlarda değildi. Mancozeb’ e tolerans tüm bölgelerde görülmüş ve tolerant izolatların yüzdesi Çarşamba’ da en düşük (%6) ve Susurluk bölgesinde en yüksek (%47.3) olmuştur. Fentin asetat’ a karşı tolerans değerlendirilme kriterine bağlı olarak değişiklik göstermiştir.

Anahtar Kelimeler:

Key words: Cercospora beticola, fentin acetate, flutriafol, fungicide resistance, mancozeb

Sensitivity of Cercospora beticola populations in Turkey to flutriafol, mancozeb, and fentin acetate

The sensitivity of sugar beet leaf spot disease caused by Cercospora beticola to fungicides used in Turkey was determined using a mycelial growth assay in petri dishes with 100 fungal isolates. The isolates were obtained from samples collected from 6 sugar beet production areas in 2005 and 2006. Flutriafol, mancozeb, and fentin acetate were added to Aspergillus complete medium (ACM) at the rates of 0.0, 0.05, 0.1, 0.5, 1.0, 5.0, and 10.0 µg mL-1, and then mycelial growth was measured. ED50 values of flutriafol for all the isolates from the sugar beet production areas ranged from 0.04 to >10.0 µg mL-1. Tolerance to this fungicide was very high in 2 regions (Adapazarı and Çarşamba) where the disease is very prevalent due to climatic conditions. In these 2 areas 74.1%, 55.5%, 81.2%, and 68.7% of the isolates grew with 5 and 10 µg mL-1 of flutriafol, respectively (1 µg mL-1 flutriafol is considered inhibitory to the growth of the fungus). In the other 4 regions included in the study, tolerance was not as high. Tolerance to mancozeb developed in all the regions; however, the percentage of tolerant isolates was lowest in Çarşamba (6%) and highest in Susurluk (47.3%). Tolerance to fentin acetate varied according to the criteria used for evaluation.

Keywords:

fentin acetate, flutriafol, fungicide resistance, mancozeb, Cercospora beticola,

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