Responses of apple tree cultivars to drought: carbohydrate composition in the leaves

Abstract: The changes in the composition of carbohydrates in the leaves of several apple cultivars were investigated during fruit development in an intensive plantation and in a model experiment. Analytical measurements were performed on leaves collected from the eastern, western, and southern sides of trees four times: on days 57, 73, 99, and 120 after flowering. Simultaneously, the shoots collected from these varieties were exposed to mild, moderate, and severe water stress induced by polyethylene-glycol solution, modeling drought. Under short-term drought, a close significant correlation (R = 0.8348) was found between the degree of leaf rolling and the carbohydrate (glucose + fructose) content 73 days after flowering. During mild drought, water-intensive cultivars exhibited significant leaf rolling and high accumulation of carbohydrates, while those with moderately rolled leaves contained less glucose and fructose. During severe drought, it is the sucrose content of leaves that can be considered a stress indicator, while during temporary dry periods, it is the changes in the glucose + fructose content of leaves. The early stage of slow fruit development was more suitable for screening the drought tolerance of apple trees based on the changes in morphology and the carbohydrate composition of the leaves. The results can be used to select apple trees with good adaptability to drought in breeding programs.

Responses of apple tree cultivars to drought: carbohydrate composition in the leaves

Abstract: The changes in the composition of carbohydrates in the leaves of several apple cultivars were investigated during fruit development in an intensive plantation and in a model experiment. Analytical measurements were performed on leaves collected from the eastern, western, and southern sides of trees four times: on days 57, 73, 99, and 120 after flowering. Simultaneously, the shoots collected from these varieties were exposed to mild, moderate, and severe water stress induced by polyethylene-glycol solution, modeling drought. Under short-term drought, a close significant correlation (R = 0.8348) was found between the degree of leaf rolling and the carbohydrate (glucose + fructose) content 73 days after flowering. During mild drought, water-intensive cultivars exhibited significant leaf rolling and high accumulation of carbohydrates, while those with moderately rolled leaves contained less glucose and fructose. During severe drought, it is the sucrose content of leaves that can be considered a stress indicator, while during temporary dry periods, it is the changes in the glucose + fructose content of leaves. The early stage of slow fruit development was more suitable for screening the drought tolerance of apple trees based on the changes in morphology and the carbohydrate composition of the leaves. The results can be used to select apple trees with good adaptability to drought in breeding programs.

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  • Bianco R, Francaviglia D (2012). Comparative responses of ‘Gala’ and ‘Fuji’ apple trees to deficit irrigation: placement versus volume effects. Plant Soil 357: 41‒58.
  • Bieleski RL (1969). Accumulation and translocation of sorbitol in apple phloem. Aust J Biol Sci 22: 611‒620.
  • Borókay R, Sárdi É (1999). Endogenous carbohydrates are good indicators of drought tolerance. Publ Univ Horticulture Food Industry 58: 63‒68.
  • Caspari HW, Einhorn TC, Preston LK, Andrews BG, Redulla CA, Lombardini L, Auvil T, McFerson JR (2004). Progress in the development of partial rootzone drying of apple trees. Acta Hort 664: 125‒132.
  • Chaves MM, Maroco JP, Pereira JS (2003). Understanding plant responses to drought—from genes to the whole plant. Funct Plant Biol 30: 239‒264.
  • El-Tayeb N (2006). Differential response of two Vicia faba cultivars to drought: growth, pigments, lipid peroxidation, organic solutes, catalase and peroxidase activity. Acta Agron Hung 54: 25‒37.
  • Escobar-Gutiérrez AJ, Zipperlin B, Carbonne F, Moing A, Gaudillère JP (1998). Photosynthesis, carbon partitioning and metabolite content during drought stress in peach seedlings. Aust J Plant Physiol 25: 197‒205.
  • Fernandez RT, Perry RL, Flore JA (1997). Drought response of young apple trees on three rootstocks. II. Gas exchange, chlorophyll fluorescence, water relations, and leaf abscisic acid. Plant Physiol Biochem 122: 841‒848.
  • Hudak I, Dobranszki J, Sárdi É, Hevesi M (2010). Changes in carbohydrate content of potato calli during osmotic stress induced by mannitol. Acta Biol Hung 61: 234‒236.
  • Jie Z, Yuncong Y, Streeter JG, Ferree DC (2010). Influence of soil drought stress on photosynthesis carbohydrates and nitrogen and phosphorus absorb in different section of leaves and stem of Fugi/M.9EML, a young apple seedling. Afri J Biotechnol 9: 5320‒5325.
  • Kadioglu A, Terzi R (2007). A dehydration avoidance mechanism: leaf rolling. Bot Rev 73: 290‒302.
  • Kadioglu A, Terzi R, Saruhan N, Saglam A (2012). Current advances in the investigation of leaf rolling caused by biotic and abiotic stress factors. Plant Sci 182: 42‒48.
  • Kadioglu A, Turgut R (1999). Some biochemical changes during leaf rolling Ctenanthe setosa (Marantaceae). Acta Physiol Plant 21: 209‒214.
  • Lakso AN, Geyer AS, Carpenter SG (1984). Seasonal osmotic relations in apple leaves of different ages. J Amer Soc Hort Sci 109: 544‒547.
  • Li TH, Li SH (2005). Leaf responses of micropropagated apple plants to water stress: nonstructural carbohydrate composition and regulatory role of metabolic enzymes. Tree Physiol 25: 495‒504.
  • Loescher WH, Everard JD (1996). Sugar alcohol metabolism in sinks and sources. In: Zamski E, Schaffer AA, editors. Photoassimilate Distribution in Plants and Crops: Source-Sink Relationships. New York, NY, USA: Marcel Dekker, pp. 185‒207.
  • Loresto GC, Chang TT, Tagumpay O (1976). Field evaluation and breeding for drought tolerance. Phillip J Crop Sci 1: 36‒39.
  • Ludlow MM (1980). Adaptive significance of stomatal responses to water stress. In: Turner NC, Kramer PJ, editors. Adaptation of Plants to Water and High Temperature Stress. New York, NY, USA: Wiley Interscience, pp. 123‒138.
  • Matthews RB, Azam-Ali SN, Peacock JM (1990). Response of four sorghum lines to midseason drought: II. Leaf characteristics. Field Crop Res 25: 297‒308.
  • Naschitz S, Naor A (2005). The effect of crop load on tree water relation consumption of ‘Golden Delicious’ apples in relation to fruit size: an operative model. J Amer Soc Hortic Sci 130: 7‒11.
  • Nemeskéri E, Sárdi É, Kovács-Nagy E, Stefanovits-Bányai É, Nagy J, Nyéki J, Szabó T (2009). Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks. Int J Hortic Sci Hung 15: 29‒36.
  • Nemeskéri E, Sárdi É, Remenyik J, Kőszegi B, Nagy P (2010a). Study of the defensive mechanism against drought in French bean (Phaseolus vulgaris L.) varieties. Acta Physiol Plant 32: 1125‒1134.
  • Nemeskéri E, Sárdi É, Szabó T, Nyéki J (2010b). Ecological drought resistance and adaptability of apple varieties. Int J Hortic Sci Hung 16: 113‒122.
  • Pelleschi S, Rocher JP, Prioul JL (1997). Effect of water restriction on carbohydrate metabolism and photosynthesis in mature maize leaves. Plant Cell Environ 20: 493‒503.
  • Ranney TG, Bassuk NL, Whitlow TH (1991). Osmotic adjustment and solute constituents in leaves and roots of water-stressed cherry (Prunus) trees. J Amer Soc Hort Sci 116: 684‒688.
  • Sárdi É, Szarka E, Csilléry G, Szarka J (2006). Biochemical examination of the general defense system of plants by OPLC. J Planar Chromatog 19: 233‒237.
  • Sárdi É, Velich I, Hevesi M, Klement Z (1999). Ontogenesis- and biotic stress-dependent variability of carbohydrate content in snap bean (Phaseolus vulgaris L.). Z Naturforsch 54c: 782‒787.
  • Sauter A, Dietz KJ, Hartung W (2002). A possible tress physiological role of abscisic acid conjugates in root-to-shoot signalling. Plant Cell Environ 25: 223‒228.
  • Singh SK, Raja Reddy K (2011). Regulation of photosynthesis, fluorescence, stomatal conductance and water-use efficiency of cowpea (Vigna unguiculata (L.) Walp.) under drought. J Photoch Photobiol B 105: 40‒50.
  • Šircelj H, Grill D, Batič F (2007). Detecting different levels of drought stress in apple trees (Malus domestica Borkh.) with selected biochemical and physiological parameters. Sci Hortic 113: 362‒369.
  • Šircelj H, Tausz M, Grill D, Batič F (2005). Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought. J Plant Physiol 162: 1308‒1318.
  • Sivaci A (2006). Seasonal changes of total carbohydrate contents in three varieties of apple (Malus sylvestris Miller) stem cuttings. Sci Hortic 109: 243‒237.
  • Vemmos SN (1995). Carbohydrate changes in flowers, leaves, shoots and spurs of Cox-Orange-Pippin apple during flowering and fruit setting periods. J Hort Sci 70: 889‒900.
  • Wang Z, Stutte GW (1992). The role of carbohydrates in active osmotic adjustment in apple under water stress. J Amer Soc Hortic Sci 117: 816‒823.
  • Xu YC, Li SH, Chai CL, Liu GJ, Chen SW (2001). Carbohydrate metabolism in source leaves of Jonagold apple trees under water stress and after water stress relief. J Fruit Sci 18: 1‒6.
  • Yokota A, Takahara K, Akashi K (2006). Water stress. In: Physiology and Molecular Biology of Stress Tolerance in Plants. Madhava Rao KV, Raghavendra AS, Janardhan Reddy K, editors. Dordrecht, the Netherlands: Springer, pp. 15‒39.
  • Zatykó I (2003). Az alma aszályérzékenységének tényezői. Kertgazdaság 35: 33‒39 (in Hungarian).
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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