Meryem İPEK, Ahmet İPEK, Philipp W. SIMON

Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition, several alleles of these locus-specific markers were associated only with certain AFLP and RAPD groups, which can allow rapid assessment of newly collected garlic accessions; therefore, these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.

Anahtar Kelimeler:

Allium sativum L., genetic characterization, DNA markers, germplasm

Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers

Keywords:

Allium sativum L., genetic characterization, DNA markers, germplasm,

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Bradley, K.F., M.A. Rieger and G.G. Collins. 1996. Classification of Australian garlic cultivars by DNA fingerprinting. Aust. J. Ex. Agric. 36: 613-618.
Dice, L.R. 1945. Measures of the amount of ecologic association between species. Ecology. 26: 297-302.
Etoh, T. and P.W. Simon. 2002. Diversity, fertility and seed production of garlic. In: Allium Crop Science: Recent Advances (Eds. H.D. Rabinowitch and L. Currah). CAB International, New York, p. 101-117.
Ipek, M., A. Ipek and P.W. Simon. 2003. Comparison of AFLPs, RAPD markers, and isozymes for diversity assessment of garlic and detection of putative duplicates in germplasm collections. J. Amer. Soc. Hort. Sci. 128: 246-252.
Ipek, M., A. Ipek, S.G. Almquist and P.W. Simon. 2005. Demonstration of linkage and development of the first low-density genetic map of garlic based on AFLP markers. Theor. Appl. Genet. 110: 228- 236.
Ipek, M., A. Ipek and P.W. Simon. 2006. Sequence homology of polymorphic AFLP markers in garlic (Allium sativum L.). Genome 49: 1246-1255.
Ipek, M., A. Ipek and P.W. Simon. 2008. Molecular characterization of Kastamonu garlic: An economically important garlic clone in Turkey. Sci. Horticult. 115: 203-208.
Karp, A., O. Seberg and M. Buiatti. 1996. Molecular techniques in the assessment of botanical diversity. Ann. Bot. 78: 143-149.
King, J.J., J.M. Bradeen, O. Bark, J.A. McCallum and M.J. Havey. 1998. A low-density genetic map of onion reveals a role for tandem duplication in the evolution of an extremely large diploid genome. Theor. Appl. Genet. 96: 52-62.
Lampasona S.G., L. Martinez and J.L. Burba. 2003. Genetic diversity among selected Argentinean garlic clones (Allium sativum L.) using AFLP (Amplified Fragment Length Polymorphism). Euphytica. 132: 115-119.
Maass, H.I. and M. Klaas. 1995. Infraspecific differentiation of garlic (Allium sativum L.) by isozyme and RAPD markers. Theor. Appl. Genet. 91: 89-97.
Nicholas, K.B. and Nicholas, H.B. Jr. 1997. Genedoc: a tool for editing and annotating multiple sequence alignments. Multiple sequence alignment and shading utility, version 2.6.002. Available from http://www.psc.edu/biomed/genedoc. [Accessed February, 2005.]
Pooler, M.R. and P.W. Simon. 1993. Characterization and classification of isozyme and morphological variation in a diverse collection of garlic clones. Euphytica. 68: 121-130.
Rohlf, F.J. 1993. NTSYS-pc, numerical taxonomy and multivariate analysis system. Exeter Publ., Setauket, New York.
Sarri, V., L. Baldoni, A. Porceddu, N.G.M. Cultrera, A. Contento, M. Frediani, A. Belaj, I. Trujillo and P.G. Cionini. 2006. Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations. Genome. 49: 1606-1615.
Simon, P.W. and M.M. Jenderek. 2003. Flowering, seed production and the genesis of garlic breeding. Plant Breed. Rev. 23: 211-244.
Volk, G.M., A.D. Henk and C.M. Richards. 2004. Genetic diversity among U.S. garlic clones as detected using AFLP methods. J. Amer. Soc. Hort. Sci. 129: 559-569.