Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica

Peronospora parasitica (Pers ex Fr.) Pers. is an obligate biotrophic pathogen that causes downy mildew in Arabidopsis thaliana (L.) Heynh. In this study, cotyledons of four A. thaliana varieties were inoculated with the Cala2 isolate of P. parasitica and the degree of susceptibility was observed under the microscope 1, 2, 3 and 7 days after inoculation (DAI). Microscopic examination of infected tissues revealed that early restriction of the pathogen was accompanied by a hypersensitive response (HR) characterised by autofluorescence under UV or blue light excitation. All haustoria, if visible at all, formed in the cells that underwent the HR in both Ws-3 and Oy-0 at all time points tested. Susceptibility was characterised by extensive pathogen growth in intercellular spaces with the formation of haustoria in both epidermal and mesophyll cells without any host response. In the Nd-1/Cala2 interaction, 100% of the haustoria formed in unresponsive cells 1 DAI; 5% of the haustoria was, however, ensheathed with callose deposits by 3 DAI in the same host, presumably because of haustorial ageing. Sexual and asexual sporulations of P. parasitica begun approximately 3 DAI. In the intermediate interaction, the deposition of callose around the haustorial body and delayed cell death were identified as the main mechanisms of resistance in the Col-0 variety.

Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica

Peronospora parasitica (Pers ex Fr.) Pers. is an obligate biotrophic pathogen that causes downy mildew in Arabidopsis thaliana (L.) Heynh. In this study, cotyledons of four A. thaliana varieties were inoculated with the Cala2 isolate of P. parasitica and the degree of susceptibility was observed under the microscope 1, 2, 3 and 7 days after inoculation (DAI). Microscopic examination of infected tissues revealed that early restriction of the pathogen was accompanied by a hypersensitive response (HR) characterised by autofluorescence under UV or blue light excitation. All haustoria, if visible at all, formed in the cells that underwent the HR in both Ws-3 and Oy-0 at all time points tested. Susceptibility was characterised by extensive pathogen growth in intercellular spaces with the formation of haustoria in both epidermal and mesophyll cells without any host response. In the Nd-1/Cala2 interaction, 100% of the haustoria formed in unresponsive cells 1 DAI; 5% of the haustoria was, however, ensheathed with callose deposits by 3 DAI in the same host, presumably because of haustorial ageing. Sexual and asexual sporulations of P. parasitica begun approximately 3 DAI. In the intermediate interaction, the deposition of callose around the haustorial body and delayed cell death were identified as the main mechanisms of resistance in the Col-0 variety.

Kaynak Göster

Bibtex @ { tbtkagriculture138664, journal = {Turkish Journal of Agriculture and Forestry}, issn = {1300-011X}, eissn = {1303-6173}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {125 - 132}, doi = {}, title = {Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica}, key = {cite}, author = {Türk, Figen Mert} }
APA Türk, F . (2002). Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica . Turkish Journal of Agriculture and Forestry , 26 (3) , 125-132 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkagriculture/issue/11645/138664
MLA Türk, F . "Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica" . Turkish Journal of Agriculture and Forestry 26 (2002 ): 125-132 <https://dergipark.org.tr/tr/pub/tbtkagriculture/issue/11645/138664>
Chicago Türk, F . "Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica". Turkish Journal of Agriculture and Forestry 26 (2002 ): 125-132
RIS TY - JOUR T1 - Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica AU - Figen Mert Türk Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Agriculture and Forestry JF - Journal JO - JOR SP - 125 EP - 132 VL - 26 IS - 3 SN - 1300-011X-1303-6173 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Agriculture and Forestry Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica %A Figen Mert Türk %T Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica %D 2002 %J Turkish Journal of Agriculture and Forestry %P 1300-011X-1303-6173 %V 26 %N 3 %R %U
ISNAD Türk, Figen Mert . "Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica". Turkish Journal of Agriculture and Forestry 26 / 3 (Mart 2002): 125-132 .
AMA Türk F . Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica. Turkish Journal of Agriculture and Forestry. 2002; 26(3): 125-132.
Vancouver Türk F . Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica. Turkish Journal of Agriculture and Forestry. 2002; 26(3): 125-132.
IEEE F. Türk , "Microscopic Evaluation of Interactions between Varieties of Arabidopsis thaliana Challenged by Peronospora parasitica", Turkish Journal of Agriculture and Forestry, c. 26, sayı. 3, ss. 125-132, Mar. 2002