Gorkem SULU, Yildiz Aka KACAR, Ilknur POLAT, Aytul KİTAPCİ, Ertugrul TURGUTOGLU, Ozhan SİMSEK, Gul SATAR

Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

Mandarin (Citrus reticulate Blanco) and lemon (C. limon L.) are among the most cultivated Citrus species in the world.Among different approaches, gamma radiation is widely used to induce mutations in citrus breeding studies. Gamma radiation causes DNA damage, randomly inducing several mutations in the genome. Molecular techniquesarewidely used to detect such induced mutations and genetic diversity in plants.In this study, simple sequence repeats (SSR), inter-simple sequence repeat (ISSR) and single-strand conformation polymorphism (SSCP) markers were used to detectthe induced mutations andgenetic diversityin someirradiated mandarin and lemon genotypes in comparison with standard citrus varieties. The irradiated genotypes used in this study derived from populationsthat had been originally obtained with gamma radiation with different gray doses applied on Yerli Mandarin and Antalya YerliYuvarlak lemon varieties. Out of 82 primers (22 SSCP, 30 ISSR and 30 SSR) tested, 55 primers (9 SSCP, 24 ISSR and 22 SSR primers) successfully amplified a total number of 363 amplicons.A single band with an approximate size of 500 nucleotides was determined using the primer ISSR 868 as differentiating between seedy parent and irradiated seedless mandarin genotypes. The polymorphism information content (PIC) values of SSCP, ISSR, and SSR markers ranged from 0.61-0.99, 0.31-0.96, and 0.33-0.96, respectively. Cluster analysis classified lemons and mandarins into 2 subgroups as mutant and commercial lemon genotypes. ISSR markers were found to be more effective to determine the genetic differences among the varieties in comparison to SSCP and SSR markers. The results clearly showed that SSCP, SSR and ISSR markers are important tools to distinguish mutant genotypes andconfirmed their usefulness for phylogenetic studies.

Keywords:

Citrus, genetic differences, mutation breeding, seedlessness, thornlessness,

PDF

___

Agarwal M, 2008, PLANT CELL REP, V27, P617, DOI 10.1007/s00299-008-0507-z
Agisimanto D, 2016, SAINS MALAYS, V45, P329
Aka-Kacar Y., 2005, BIOLOGIA, V60, P295 .
Amar MH, 2011, SCI HORTIC-AMSTERDAM, V128, P220, DOI 10.1016/j.scienta.2011.01.021
Atak C, 2004, MUTAT RES-FUND MOL M, V556, P35, DOI 10.1016/j.mrfmmm.2004.06.037
Barkley NA, 2006, THEOR APPL GENET, V112, P1519, DOI 10.1007/s00122-006-0255-9
Barkley NA, 2009, PLANT SYST EVOL, V282, P71, DOI 10.1007/s00606-009-0208-2
Bermejo A, 2012, SPAN J AGRIC RES, V10, P768, DOI 10.5424/sjar/2012103-460-11
BERMEJO A, 2011, FOOD NUTR SCI, P00169, DOI [DOI 10.4236/FNS.2011.23024, DOI 10.4236/fns.2011.23024]
Biswas MK, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0104182
Biswas MK, 2011, SCI HORTIC-AMSTERDAM, V129, P798, DOI 10.1016/j.scienta.2011.06.015
Cameron J., 1968, CITRUS IND, VVol.II, P325
Campos ET, 2005, INTERCIENCIA, V30, P687
Capparelli R, 2004, BIOTECHNOL LETT, V26, P1295, DOI 10.1023/B:BILE.0000044920.36497.fc
Carrillo-Medrano S. H., 2018, Revista Mexicana de Ciencias Agricolas, V9, P11
Chapot H, 1975, CITRUS, P6 .
Chen CX, 2008, TREE GENET GENOMES, V4, P1, DOI 10.1007/s11295-007-0083-3
Cheng ChangFeng, 2008, Southwest China Journal of Agricultural Sciences, V21, P1378
Christov N. K., 2004, GENETIKA, V36, P47
Crowley JR, 2011, THESIS .
Degirmenci D, 2008, MUSTAFA KEMAL U ZIRA, V13, P13
DENG ZN, 1995, J HORTIC SCI, V70, P117, DOI 10.1080/14620316.1995.11515281
DICE LR, 1945, ECOLOGY, V26, P297, DOI 10.2307/1932409
Donini B., 1982, Induced mutations in vegetatively propagated plants II. Coimbatore, India, 11-15 February 1980, P29
Doyle J. J., 1990, FOCUS, V12, P13 .
Duran C, 2009, CURR BIOINFORM, V4, P16, DOI 10.2174/157489309787158198
Fang DQ, 1997, THEOR APPL GENET, V95, P408, DOI 10.1007/s001220050577
Federici CT, 1998, THEOR APPL GENET, V96, P812, DOI 10.1007/s001220050807
Fu J, 2011, PLANT BREEDING, V130, P287, DOI 10.1111/j.1439-0523.2010.01773.x
FUJITA K, 1994, PCR METH APPL, V4, pS137
Garcia-Lor A, 2015, TREE GENET GENOMES, V11, DOI 10.1007/s11295-015-0951-1
Goldenberg L., 2014, Food and Nutrition Sciences, V5, P943, DOI 10.4236/fns.2014.510105
Gulsen O, 2007, SCI HORTIC-AMSTERDAM, V112, P184, DOI 10.1016/j.scienta.2006.12.040
Gulsen O, 2001, J AM SOC HORTIC SCI, V126, P309, DOI 10.21273/JASHS.126.3.309
Gulsen O, 2010, EUPHYTICA, V173, P265, DOI 10.1007/s10681-010-0146-7
HEARN CJ, 1984, J AM SOC HORTIC SCI, V109, P270
HENSZ R A, 1985, Journal of the Rio Grande Valley Horticultural Society, V38, P75
Hodgson R.W., 1967, CITRUS IND, V1, P431
[黄建昌 Huang Jianchang], 2003, [核农学报, Acta Agriculturae Nucleatae Sinica], V17, P171
Huang JH, 2017, SCI HORTIC-AMSTERDAM, V217, P73, DOI 10.1016/j.scienta.2017.01.034
Hussein EHA, 2003, ARAB J BIOTECHNOL, V6, P203
Kacar Y, 2013, J FOOD AGRIC ENVIRON, V11, P516
Kacar YA, 2014, TURK J AGRIC FOR, V38, P340, DOI 10.3906/tar-1301-15
Kajiwara H, 2005, RICE GENETICS NEWSLE, V20, P1
Karatas DD, 2010, GENET MOL RES, V9, P2357, DOI 10.4238/vol9-4gmr864
Khalil SA, 2011, AFR J BIOTECHNOL, V10, P14562
Konstantinos KV, 2008, MOL BIOTECHNOL, V38, P155, DOI 10.1007/s12033-007-9006-7
Lamo K., 2017, INT J CURR MICROBIOL, V6, P3620, DOI DOI 10.20546/ijcmas.2017.612.418
Lombardo G, 2012, PLANT BIOSYST, V146, P19, DOI 10.1080/11263504.2011.557101
Lonnig W. E., 2005, RECENT RES DEV GENET, V2, P45
Mabberley D., 2008, MABBERLEYS PLANT BOO
Mahmoudzadeh H, 2013, THESIS .
Mallick M, 2017, INDIAN J BIOTECHNOL, V16, P244
Nadeem MA, 2018, BIOTECHNOL BIOTEC EQ, V32, P261, DOI 10.1080/13102818.2017.1400401
Nicolosi E, 2000, THEOR APPL GENET, V100, P1155, DOI 10.1007/s001220051419
Pestanana RKN, 2011, EUPHYTICA, V178, P151, DOI 10.1007/s10681-010-0329-2
ORITA M, 1989, P NATL ACAD SCI USA, V86, P2766, DOI 10.1073/pnas.86.8.2766
Pal D, 2013, AGR RES, V2, P236, DOI 10.1007/s40003-013-0072-8
Polat I, 2015, DERIM, V32, P47 .
Polat I, 2018, ADV PLANT BREEDING S, V3, P43
Polat I, 2015, PAK J BOT, V47, P1095 .
Powell W, 1996, TRENDS PLANT SCI, V1, P215, DOI 10.1016/1360-1385(96)86898-1
Raza H., 2003, INT J AGRIC BIOL, V5, P388
Roose M. L., 2010, P INT SOC CITRICULTU, V1, P202
Rozen S, 2000, Methods Mol Biol, V132, P365
Russo F, 1981, P INT SOC CITRICULTU, P68
Sankar AA, 2001, THEOR APPL GENET, V102, P206, DOI 10.1007/s001220051637
Sato Y, 2003, THEOR APPL GENET, V107, P560, DOI 10.1007/s00122-003-1282-4
Scarano MT, 2002, PLANT CELL REP, V20, P1162, DOI 10.1007/s00299-002-0450-3
Shahsavar AR, 2007, SCI HORTIC-AMSTERDAM, V112, P310, DOI 10.1016/j.scienta.2006.12.039
Simsek O, 2011, P 2 CITR BIOT ACT HO, V892
Smith JSC, 1997, THEOR APPL GENET, V95, P163, DOI 10.1007/s001220050544
Starrantino A, 1988, 6 INT CITR C P, P231
Stuber CW, 1999, CROP SCI, V39, P1571, DOI 10.2135/cropsci1999.3961571x
Sutarto I., 2009, Induced plant mutations in the genomics era. Proceedings of an International Joint FAO/IAEA Symposium, 2008, P306
Swingle WT, 1967, CITRUS IND, P389 .
Tanaka T, 1977, STUDIA CITROLOGICA, V14, P1
Tang XL, 1994, P SER, P748 .
Terol JF, 2008, BMC GENOMICS, V9, P12 .
Turgutoglu E, 2014, ARASTIRMA PROJELERI, P34
Usman M, 2018, IEEE INT SYMP SIGNAL, P291, DOI 10.1109/ISSPIT.2018.8642675
Uzun A, 2010, CZECH J GENET PLANT, V46, P170, DOI 10.17221/89/2010-CJGPB
Uzun A, 2011, PLANT MOL BIOL REP, V29, P693, DOI 10.1007/s11105-010-0277-y
Uzun B, 2003, PLANT BREEDING, V122, P95, DOI 10.1046/j.1439-0523.2003.00787.x
Wang C T, 2001, Yi Chuan Xue Bao, V28, P852
Yadav BR, 2009, GENETICS GENOMICS GE .
Yoshida T, 1999, J JPN SOC HORTIC SCI, V68, P1104, DOI 10.2503/jjshs.68.1104
Zhang SJ, 2017, HORTIC RES-ENGLAND, V4, DOI 10.1038/hortres.2017.41