Effect of mycorrhizal inoculation on growth, nitrogen fixation, and nutrient uptake in Cicer arietinum (L.) under salt stress
Most legumes in natural conditions form a symbiosis with arbuscular mycorrhizal (AM) fungi. AM fungi in saline soils have been reported to improve salinity tolerance and growth in plants. In the present study, interaction between mycorrhizal fungus, Glomus mosseae, and salinity stress in relation to plant growth, nitrogen fixation, and nutrient accumulation was evaluated in Cicer arietinum (L.) (chickpea). Two genotypes of chickpea (Pusa-329, salt tolerant, and Pusa-240, salt sensitive) were compared under different levels of salinity with and without mycorrhizal inoculations. Salt stress resulted in a noticeable decline in shoot and root dry matter accumulation, resulting in a decline in the shoot-to-root ratio (SRR) in all plants. However, Pusa-329 was found to be more tolerant to salinity than Pusa-240. AM plants exhibited better growth and biomass accumulation under stressed as well as unstressed conditions. Mycorrhizal infection (MI) was reduced with increasing salinity levels, but the mycorrhizal dependency (MD) increased, which was more evident in Pusa-240. Salinity resulted in a marked decline in the nodule dry weights, whereas a surge in the nodule number was recorded. Nitrogenase activity was reduced with increasing salt concentrations. AM plants had considerably higher nodule numbers, dry weights, and nitrogenase activity under both saline and nonsaline environments. Pusa-329 had a comparatively lower Na+ concentration and higher K+ and Ca2+ concentrations than Pusa-240. Although nitrogen (N) and phosphorus (P) contents declined with increasing salinity, Pusa-329 had higher levels of N and P as compared with Pusa-240. Plants inoculated with Glomus mosseae had better plant growth and nitrogen fixation under salt stress.
Effect of mycorrhizal inoculation on growth, nitrogen fixation, and nutrient uptake in Cicer arietinum (L.) under salt stress
Most legumes in natural conditions form a symbiosis with arbuscular mycorrhizal (AM) fungi. AM fungi in saline soils have been reported to improve salinity tolerance and growth in plants. In the present study, interaction between mycorrhizal fungus, Glomus mosseae, and salinity stress in relation to plant growth, nitrogen fixation, and nutrient accumulation was evaluated in Cicer arietinum (L.) (chickpea). Two genotypes of chickpea (Pusa-329, salt tolerant, and Pusa-240, salt sensitive) were compared under different levels of salinity with and without mycorrhizal inoculations. Salt stress resulted in a noticeable decline in shoot and root dry matter accumulation, resulting in a decline in the shoot-to-root ratio (SRR) in all plants. However, Pusa-329 was found to be more tolerant to salinity than Pusa-240. AM plants exhibited better growth and biomass accumulation under stressed as well as unstressed conditions. Mycorrhizal infection (MI) was reduced with increasing salinity levels, but the mycorrhizal dependency (MD) increased, which was more evident in Pusa-240. Salinity resulted in a marked decline in the nodule dry weights, whereas a surge in the nodule number was recorded. Nitrogenase activity was reduced with increasing salt concentrations. AM plants had considerably higher nodule numbers, dry weights, and nitrogenase activity under both saline and nonsaline environments. Pusa-329 had a comparatively lower Na+ concentration and higher K+ and Ca2+ concentrations than Pusa-240. Although nitrogen (N) and phosphorus (P) contents declined with increasing salinity, Pusa-329 had higher levels of N and P as compared with Pusa-240. Plants inoculated with Glomus mosseae had better plant growth and nitrogen fixation under salt stress.
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- Abdelly C, Krouma A, Drevon J (2005) Nitrogen fi xation and yield of chickpea in saline Mediterranean zones. In: Th e Grain
- Legumes, Vol. 42, IP (FOOD-CT-2004-506223).
- Ahmad F, Gaur P, Croser J (2005) Chickpea (Cicer arietinum L.).
- In: Genetic Resources, Chromosome Engineering and Crop Improvement – Grain Legumes, Vol. 1 (Eds. R Singh, P Jauhar). CRC Press, Boca Raton, Florida, pp. 185-214. Al-Karaki GN, Hammad R (2001) Mycorrhizal infl uence on fruit yield and mineral content of tomato grown under salt stress. J Plant Nutr 24: 1311-1323.
- Al-Raddad A (1991) Response of bean, broad bean and chickpea plants to inoculation with Glomus species. Scientia Hort 146: 200.
- Amora-Lazcano E, Vazquez MM, Azcon R (1998) Response of nitrogen-transforming microorganisms to arbuscular mycorrhizal fungi. Biol Fertil Soils 27: 65-70.
- Ashraf M, McNeilly T (2004) Salinity tolerance in Brassica oilseeds.
- Crit Rev Plant Sci 23: 157-174. Balibrea ME, Cuartero J, Bolarín JC, Perez-Alfocea F (2003) Activities during fruit development of Lycopersicon genotypes diff ering in tolerance salinity. Physiol Plant 118: 38-46.
- Chapman HD, Pratt PF (1961) Methods of Analysis for Soil, Plant and Waters. Division of Agricultural Sciences, University of California, Berkeley.
- Cordovilla MP, Ligero F, Lluch C (1999) Eff ects of NaCl on growth and nitrogen fi xation and assimilation of inoculated and KNO3 fertilized Vicia faba L. and Pisum sativum L. plants. Plant Sci : 127-136.
- Duncan DB (1955) Multiple range and multiple F-tests. Biometrics : 1-42. Control Control dS m 1 dS m 1+A M dS m-1 m 1 dS dS m 1 Pusa-329 Pusa-240