In this study, the genetic diversity of 90 avocado (Persea americana Mill) cultivars from Antioquia (Colombia) was compared to 67 germplasm collection accessions using 14 microsatellites. An average of 4.32 ± 2.0 alleles per locus was found, as observed in previous studies. The expected and observed heterozygosity ranges were 0.384-0.724 and 0.393-0.686, respectively. The Antioquian avocados were genetically structured according to an analysis of molecular variance test (fixation index (FST) = 0.054, P