Holstein İneklerde GH/AluI Polimorfizmi ile Bazı Süt Verim Özellikleri Arasındaki İlişkiler
Bu çalışmanın amacı, organik yetiştirilen 115 baş Holstein ırkı inekten oluşan sürüde Büyüme Hormon (GH) geni bakımından genotip ve allel frekanslarınının dağılımını belirlemek, genetik varyasyonu ortaya koymak ve bazı süt verim özellikleri arasındaki ilişkileri araştırmaktır. Elde edilen bulgular sonucunda, çalışılan sığır populasyonunun GH geni LL, LV ve VV genotip frekanslarının sırasıyla 0,41, 0,49, ve 0,10 olarak bulunduğu ve Hardy-Weinberg eşitliği testine göre dengede olduğu görülmüştür. Laktasyon süt verimi ortalamaları LL, LV ve VV genotipleri için sırasıyla 7136 kg, 7470 kg ve 8017 kg olarak tespit edilmiştir. Genotiplere ait incelenen Laktasyon süt verimi, 305 günlük süt verimi, Pik süt verimi ve Laktasyon uzunluğu ortalamaları arasında istatistiksel olarak anlamlı ilişki bulunamamıştır. Çalışmada GH/AluI polimorfizminin sığırların süt verim özelliklerinin ıslahı için tek başına yeterli olmadığı görülmüştür
Relationships between GH/AluI Polymorphism and Some Performance Traits in Holstein Cows
The aim of this study is to determine the genotype and allelic frequencies of Growth Hormone (GH) gene, to present the genetic variation, and to research the relationships between the GH genotypes and some milk production traits of 115 organically grown Holstein cows. It was seen that GH gene LL, LV ve VV genotype frequencies is 0.41, 0.49, ve 0.10 respectively, and is stable according to Hardy-Weinberg equilibrium genetic balance test in the cattle population. Moreover, Lactation milk yield averages were found to be 7136 kg, 7470 kg and 8017 kg for the LL, LV and VV genotypes, respectively. There was no statistically significant relationship between the actual lactation milk yield, 305d milk yield, peak daily yield, lactation length averages of different genotypes. In the study, GH/AluI polymorphism was found to be insufficient for improvement of the milk yield traits alone
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- Bass JJ, Oldham JM, Hodgkinson SC, Fowke PJ, Sauerwein H,
Molan P, Breier BH, Gluckman PD. 1991. Influence of
nutrition and bovine growth hormone (GH) on hepatic GH
binding, insulin-like growth factor-I and growth of lambs. J.
Endocrinol, 128(2): 181-186.
- Bauman DE. 1992. Bovine somatotropin: review of an emerging
animal technology. J. Dairy Sci. 75 (12): 3432-3451.
- Chrenek P, Kmet J, Sakowski T, Vasicek D, Huba J, Chrenek J.
1998. Relationships of growth hormone genotypes with
meat production traits of Slovak Pied bulls. Czech J. Anim.
Sci., 43(12): 541-544.
- Drogemuller C, Hamann H, Distl O. 2001. Candidate gene
markers for litter size in different German pig lines. J. Anim.
Sci., 79: 2565-2570.
- Dybus A. 2002. Associations between Leu/Val polymorphism of
growth hormone gene and milk production traits in Blackand-White cattle. Arch. Tierzucht Dummerstorf, 45 (5): 421-
428.
- Falaki M, Gengler N, Sneyers M, Prandi A, Massart S,
Formigoni A, Burny A, Portetelle D, Renaville R. 1996.
Relationships of polymorphisms for growth hormone and
growth hormone receptor genes with milk production traits
for Italian Holstein-Friesian bulls. J. Dairy Sci.79(8):1446-
1453.
- Fries R, Eggen A, Womack JE. 1993. The bovine genome map.
Mamm. Genome., 4: 405-428.
- Ge W, Davis ME, Hines HC, Irvin KM, Simmen CM. 2003.
Association of single nucleotide polymorphisms in the
growth hormone and growth hormone receptor genes with
serum insulin-like growth factor I concentration and growth
traits in Angus cattle. J. Anim. Sci., 81: 641-648.
- Grochowska R, Zwierzchowski L. Snochowski M, Reklewski Z.
1999. Stimulated growth hormone (GH) release in Friesian
cattle with respect to GH genotypes. Reproduction Nutrition
Development 39 (2): 171-180. Abst.
- Grochowska R, Lunden A, Zwierzchowski L, Snochowski M,
Oprzadek J. 2001. Association between gene polymorphism
of growth hormone and carcass traits in dairy bulls. Anim.
Sci., 72: 441-447.
- Kovacs K, Volgyi-Csik J, Zsolnai A, Gyorkos I, Fesus L. 2006.
Associations between the AluI polymorphism of growth
hormone gene and production and reproduction traits in a
Hungarian Holstein-Friesian bull dam population. Arch.
Anim. Breed., 49(3): 236-249.
- Lucy MC, Hauser SD, Eppard PJ, Krivi GG, Clark JH, Bauman
DE, Collier RJ. 1993. Variants of somatotropin in cattlegene frequencies in major dairy breeds and associated milkproduction. Domest. Anim. Endocrinol., 10(4): 325-333.
Abst.
- Özdemir M. 2011. Determination of allele frequencies of growth
hormone AluI polymorphism in Brown Swiss, Holstein,
native East Anatolian Red and Turkish Grey breeds.
International Journal of Dairy Technology. 64(3): 350-352.
- Ozdemir M, Topal M, Aksakal V. 2018. The relationships
between performance traits and the bGH/AluI and Pit1/HinfI polymorphisms in Holstein cows. Indian J. Anim.
Res., 52(2): 186-191.
- Özkan E, Soysal Mİ, Dinç H, Sönmez G, Okyar M, Togan İ.
2009. Türkiye sığır ırklarında büyüme hormonu AluI ve
MspI polimorfizminin PZR-RFLP yönteminin kullanılarak
belirlenmesi. 6. Ulusal Zootekni Kongresi, 24-26 Haziran,
Erzurum, 93-102.
- Schlee P, Graml R, Rottmann O, Pirchner F. 1994. Influence of
growth hormone genotypes on breeding values of
Simmental bulls. Journal of Animal Breeding and Genetics,
111: 253-256.
- Sejrsen K, Foldager J, Sorensen MT, Akers RM, Bauman DE.
1986. Effect of exogenous bovine somatotropin on pubertal
mammary development in heifers. J. Dairy Sci. 69(6): 1528-
1535.
- Sönmez Z, Özdemir M, Aksakal V. 2017. Büyüme Hormonu
Geni Polimorfizminin Buzağı Doğum Ağırlığı Üzerine
Etkisi. Türk Tarım-Gıda Bilim ve Teknoloji Dergisi, 5(13):
1781-1784.
- Tambasco DD, Paz CCP, Tambasco-Studart MD, Pereira AP,
Alencar MM, Freita AR, Coutinho LL, Packer IU, Regitano
- LCA. 2003. Candidate genes for growth traits inbeef cattle
crosses Bos taurus x Bos indicus. J Anim. Breed. Genet.,
120: 51-56.
- Unanian MM, Barreto CC, Freitas AR, Cordeiro CMT,
Josahkian LA. 2000. Association of growth hormone gene
polymorphisms with weight traits in Nelore breed. Rev.
Bras. Zootec. 29: 1380-1386.
- Vasconcellos LPMK, Tambasco DD, Pereira AP, Coutinho LL,
Regitano LCA. 2003. Genetic characterization of Aberdeen
Angus cattle using molecular markers. Genetics and
Molecular Biology. 26(2): 133-137.
- Yao J, Aggrey SE, Zadworny D, Hayes JF, Kuhnlein U. 1996.
Sequence variations in the bovine growth hormone gene
characterized by single-strand conformation polymorphins
(SSCP) analysis and their association with milk production
traits in Holsteins. Genetics., 144: 1809-1816.