Gamze GÖGER, Burcu AKÇAL ÇOMOĞLU, Gökalp İŞCAN, Fatih DEMİRCİ

Uçucu yağların standart antifungal maddeler ile kombinasyonları, sentetik ilaçların olumsuz etkilerini azaltabilir ve kandidiyaz dahil olmak üzere çeşitli patolojilere karşı alternatif bir yaklaşım olarak kullanılabilir. Bu çalışmanın amacı, Farmakope kalitesindeki Lavanta (Lavandula angustifolia Miller) uçucu yağlarının ketokonazol ile kombinasyonlarının patojen Candida albicans ATCC 10231, C. krusei NRRL Y-7179 ve C. glabrata ATCC 66032 suşlarına karşı antikandidal etkinliğini belirlemektir. İncelenen uçucu yağların kimyasal içerikleri hem gaz kromatografisi/alev iyonlaşma dedektörü (GK / AİD) hem de gaz kromatografisi/kütle spektrometresi (GK/KS) yöntemleriyle belirlenmiştir. Esansiyel yağların antikandidal aktiviteleri, mikrodilüsyon metodu ile in vitro olarak incelenmiştir. Lavanta uçucu yağları ve ketokonazol kombinasyon çalışmaları dama tahtası yöntemi kullanılarak test edilmiştir. Lavanta uçucu yağları ile ketokonazol kombinasyon çalışması sonuçları, test edilen tüm Candida suşlarına karşı “aditif” ve “indiferent” etkili olduğu görülmüştür. Sonuç olarak, lavanta uçucu yağlarının ketokonazol kombinasyonu ile gözlemlenen etkiler, patojenik Candida suşlarına karşı kullanımda etkili olabilir.

EVALUATION OF ANTICANDIDAL EFFECTS OF ESSENTIAL OILS OF COMMERCIAL LAVENDER (Lavandula angustifolia Miller) IN COMBINATION WITH KETOCONAZOLE AGAINTS SOME Candida Berkhout STRAINS

Application of combination studies with essential oils and standard antifungal drugs may reduce adverse effects of synthetic drugs and serve as alternative approach against various pathologies including candidiasis. The aim of the present study was to determine the interaction of two commercial lavender (Lavandula angustifolia Miller) essential oils in combination with ketoconazole against clinical and standard strains of the human pathogens Candida albicans ATCC 10231, C. krusei NRRL Y-7179 and C. glabrata ATCC 66032. The chemical compositions of the investigated essential oils were confirmed both by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) methods. Anticandidal activities of the essential oils were screened in vitro by the microdilution method. The resulting interaction of combining lavender essential oils and ketoconazole was tested using the checkerboard method. The results showed that the interaction between essential oils and ketoconazole revealed additive and indifferent effects against the tested strains. In conclusion, the effects observed by the combination of lavender essential oils and ketoconazole may be encouraging to be used against pathogenic Candida strains.

Keywords:

Lavender (Lavandula angustifolia) Candida albicans, Candida glabrata, Ketoconazole, Candida krusei,

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